公开(公告)号：US07024312B1

公开(公告)日：2006-04-04

申请号：US09618579

申请日：2000-07-18

Applicant: Sergey A. Selifonov ,  Willem P. C. Stemmer ,  Claes Gustafsson ,  Matthew Tobin ,  Stephen del Cardayre ,  Phillip A. Patten ,  Jeremy Minshull ,  Lorraine J. Giver

Inventor： Sergey A. Selifonov ,  Willem P. C. Stemmer ,  Claes Gustafsson ,  Matthew Tobin ,  Stephen del Cardayre ,  Phillip A. Patten ,  Jeremy Minshull ,  Lorraine J. Giver

IPC: G01N33/48 ,  C12Q1/00 ,  C12Q1/68 ,  C07H21/02 ,  C07K1/00

CPC classification number: G06F19/16 ,  B01J2219/007 ,  C40B50/02 ,  G06F19/14 ,  G06F19/18 ,  G06F19/22 ,  G06F19/24

Abstract: “In silico” nucleic acid recombination methods, related integrated systems utilizing genetic operators and libraries made by in silico shuffling methods are provided.

公开(公告)号：US06528311B1

公开(公告)日：2003-03-04

申请号：US09516051

申请日：2000-03-01

Applicant: Stephen delCardayre ,  Matthew Tobin ,  William P. C. Stemmer ,  Jon E. Ness ,  Jeremy Minshull ,  Phillip Patten ,  Venkiteswaran Subramanian ,  Linda Castle ,  Claus M. Krebber ,  Steve Bass

Inventor： Stephen delCardayre ,  Matthew Tobin ,  William P. C. Stemmer ,  Jon E. Ness ,  Jeremy Minshull ,  Phillip Patten ,  Venkiteswaran Subramanian ,  Linda Castle ,  Claus M. Krebber ,  Steve Bass

IPC: C12N1500

CPC classification number: C12N15/102 ,  C07K14/245 ,  C12N15/1027 ,  C12N15/8271 ,  C12N15/8273 ,  C12N15/8279 ,  C12P17/162 ,  C12Q1/68 ,  C12Q1/6811 ,  C12Q1/6827 ,  C12R1/465

Abstract: The invention provides methods employing iterative cycles of recombination and selection/screening for evolution of whole cells and organisms toward acquisition of desired properties. Examples of such properties include enhanced recombinogenicity, genome copy number, and capacity for expression and/or secretion of proteins and secondary metabolites.

公开(公告)号：US06352859B1

公开(公告)日：2002-03-05

申请号：US09626343

申请日：2000-07-26

Applicant: Stephen delCardayre ,  Matthew Tobin ,  William P. C. Stemmer ,  Jon E. Ness ,  Jeremy Minshull ,  Phillip Patten ,  Venkiteswatan Subramanian ,  Linda Castle ,  Claus M. Krebber ,  Steve Bass

Inventor： Stephen delCardayre ,  Matthew Tobin ,  William P. C. Stemmer ,  Jon E. Ness ,  Jeremy Minshull ,  Phillip Patten ,  Venkiteswatan Subramanian ,  Linda Castle ,  Claus M. Krebber ,  Steve Bass

IPC: C12N1500

CPC classification number: A01H1/00 ,  A01K2217/05 ,  C12N15/1027 ,  C12N15/8271 ,  C12N15/8273 ,  C12N15/8279 ,  C12Q1/6811

Abstract: The invention provides methods employing iterative cycles of recombination and selection/screening for evolution of whole cells and organisms toward acquisition of desired properties. Examples of such properties include enhanced recombinogenicity, genome copy number, and capacity for expression and/or secretion of proteins and secondary metabolites.

公开(公告)号：US06335198B1

公开(公告)日：2002-01-01

申请号：US09626047

申请日：2000-07-26

Applicant: Stephen delCardayre ,  Matthew Tobin

Inventor： Stephen delCardayre ,  Matthew Tobin

IPC: C12N1500

CPC classification number: C12N15/102 ,  C07K14/245 ,  C12N15/1027 ,  C12N15/8271 ,  C12N15/8273 ,  C12N15/8279 ,  C12P17/162 ,  C12Q1/68 ,  C12Q1/6811 ,  C12Q1/6827 ,  C12R1/465

Abstract: The invention provides methods employing iterative cycles of recombination and selection/screening for evolution of whole cells and organisms toward acquisition of desired properties. Examples of such properties include enhanced recombinogenicity, genome copy number, and capacity for expression and/or secretion of proteins and secondary metabolites.

Abstract translation: 本发明提供了采用迭代循环重组和选择/筛选全细胞和生物体以获得所需性质的方法的方法。 这些性质的实例包括增加的重组原性，基因组拷贝数以及蛋白质和次级代谢物的表达和/或分泌能力。