公开(公告)号：US08080375B2

公开(公告)日：2011-12-20

申请号：US11914697

申请日：2006-05-18

Applicant: Stuart Wilson ,  John Christopher Stanley ,  Sharon Banin

Inventor： Stuart Wilson ,  John Christopher Stanley ,  Sharon Banin

IPC: C12Q1/68

CPC classification number: G01N33/57434 ,  C12Q1/42 ,  C12Q1/682 ,  G01N33/573 ,  C12Q2563/179 ,  C12Q2521/525

Abstract: An improved method of detecting an enzyme in a sample, which enzyme is capable of adding or removing a chemical moiety to or from a nucleic acid molecule, thereby conferring the nucleic acid molecule with the ability to be extended to generate a novel detectable nucleic acid molecule, comprises the steps of allowing the sample to be tested for the presence of the enzyme to interact with the nucleic acid molecule; and testing for interaction of the enzyme with the nucleic acid molecule by detecting the novel nucleic acid molecule generated only in the presence of the enzyme. The preferred enzyme is a phosphatase. The methods have a number of applications, for example in enhancing the sensitivity of immunoassays, for detecting pathogen associated phosphatase, for diagnosing certain conditions and for detecting specific contaminants in a sample.

Abstract translation: 一种检测样品中酶的改进方法，该酶能够向或从核酸分子添加或除去化学部分，从而赋予具有延伸以产生新的可检测核酸分子的能力的核酸分子 包括以下步骤：使样品测试酶的存在与核酸分子相互作用; 并通过检测仅在酶存在下产生的新的核酸分子来测试酶与核酸分子的相互作用。 优选的酶是磷酸酶。 该方法具有许多应用，例如增强免疫测定的灵敏度，用于检测病原体相关磷酸酶，用于诊断某些条件并检测样品中的特定污染物。

公开(公告)号：US07612079B2

公开(公告)日：2009-11-03

申请号：US10742237

申请日：2003-12-18

Applicant: Peter Martin Fischer ,  Mike Jarman ,  Ted McDonald ,  Bernard Nutley ,  Florence Raynaud ,  Stuart Wilson ,  Paul Workman

Inventor： Peter Martin Fischer ,  Mike Jarman ,  Ted McDonald ,  Bernard Nutley ,  Florence Raynaud ,  Stuart Wilson ,  Paul Workman

IPC: C07D473/16 ,  A61K31/52 ,  A61P35/00 ,  C07C215/08 ,  A61P17/06 ,  A61P35/02

CPC classification number: C07D473/16

Abstract: The present invention relates to compounds of formula I or a pharmaceutically acceptable salt thereof wherein R2 is 2-hydroxymethylpyrrolidin-1-yl, or NHCH(R4)CH(R3)OH, wherein R3 is hydrogen or methyl and R4 is methyl, ethyl or isopropyl; R6 is 3-nitrophenylamino, 3,4-dimethoxybenzylamino, 3-iodobenzyl-amino, pyrid-2-yl-methylamino, pyrid-4-yl-methylamino or indan-5-amino; R9 is isopropyl or cyclopentanyl. In a further aspect, the invention relates to pharmaceutical compositions comprising said compounds, and the use thereof in treating antiproliferative disorders and or viral disorders.

Abstract translation: 本发明涉及式I化合物或其药学上可接受的盐，其中R2是2-羟甲基吡咯烷-1-基或NHCH（R4）CH（R3）OH，其中R3是氢或甲基，R4是甲基，乙基或 异丙基 R6是3-硝基苯基氨基，3,4-二甲氧基苄基氨基，3-碘苄基 - 氨基，吡啶-2-基 - 甲基氨基，吡啶-4-基 - 甲基氨基或茚满-5-氨基; R9是异丙基或环戊基。 在另一方面，本发明涉及包含所述化合物的药物组合物及其在治疗抗增生性疾病和/或病毒性疾病中的用途。

公开(公告)号：US07582642B2

公开(公告)日：2009-09-01

申请号：US11033692

申请日：2005-01-11

Applicant: Peter Martin Fischer ,  Michael Jarman ,  Edward McDonald ,  Bernard Nutley ,  Florence Raynaud ,  Stuart Wilson ,  Paul Workman

Inventor： Peter Martin Fischer ,  Michael Jarman ,  Edward McDonald ,  Bernard Nutley ,  Florence Raynaud ,  Stuart Wilson ,  Paul Workman

IPC: C07D473/16 ,  A61K31/52 ,  A61P35/00 ,  A61P31/14 ,  A61P31/20 ,  C07D473/40

CPC classification number: C07D473/16 ,  A61K31/52 ,  Y02A50/411

Abstract: The present invention relates to compounds of formula 1 or pharmaceutically acceptable salts thereof, wherein one of R1 and R2 is methyl, ethyl or isopropyl, and the other is H; R3 and R4 are each independently H, branched or unbranched C1-C6 alkyl, or aryl, and wherein at least one of R3 and R4 is other than H; R5 is a branched or unbranched C1-C5 alkyl group or a C1-C6 cycloalkyl group, each of which may be optionally substituted with one or more OH groups; R6, R7, R8 and R9 are each independently H, halogen, NO2, OH, OMe, CN, NH2, COOH, CONH2, or SO2NH2. A further aspect of the invention relates to pharmaceutical compositions comprising compounds of formula 1, and the use of said compounds in treating proliferative disorders, viral disorders, stroke, alopecia, CNS disorders, neurodegenerative disorders, or diabetes.

Abstract translation: 本发明涉及式1化合物或其药学上可接受的盐，其中R 1和R 2之一是甲基，乙基或异丙基，另一个是H; R 3和R 4各自独立地为H，支链或非支链的C 1 -C 6烷基或芳基，并且其中R 3和R 4中的至少一个不是H; R5是支链或非支链C 1 -C 5烷基或C 1 -C 6环烷基，其各自可以任选被一个或多个OH基取代; R6，R7，R8和R9各自独立地为H，卤素，NO2，OH，OMe，CN，NH2，COOH，CONH2或SO2NH2。 本发明的另一方面涉及包含式1化合物的药物组合物，以及所述化合物在治疗增殖性疾病，病毒性疾病，中风，脱发，中枢神经系统疾病，神经变性疾病或糖尿病中的用途。

公开(公告)号：US20090215047A1

公开(公告)日：2009-08-27

申请号：US11993986

申请日：2006-07-03

Applicant: Stuart Wilson ,  Sharon Banin

Inventor： Stuart Wilson ,  Sharon Banin

IPC: C12Q1/68

CPC classification number: C12Q1/25 ,  C12Q1/68 ,  C12Q1/6844 ,  Y02A50/52 ,  C12Q2563/143 ,  C12Q2561/125 ,  C12Q2521/543

Abstract: A method of detecting a molecule associated with viability of one or more cells or organisms in a sample comprises the initial step of contacting the sample with an enzyme, which enzyme is capable of adding or removing a chemical moiety to or from a nucleic acid molecule in the presence of the molecule associated with viability of the of the one or more cells or organisms. This thereby generates a novel detectable nucleic acid molecule. The next step involves detecting the presence of the molecule associated with viability of the one or more cells or organisms by detecting the novel nucleic acid molecule generated only in the presence of the molecule associated with viability of the one or more cells or organisms. A most preferred molecule associated with viability is ATP, although NAD may also be detected. A preferred enzyme for use in the methods is ligase. The method has numerous applications, in particular in monitoring viability of cells, toxicology testing and determining whether there is contamination in a sample or on a surface. Kits are also provided for carrying out the methods.

Abstract translation: 检测与样品中一种或多种细胞或生物体的活力相关的分子的方法包括使样品与酶接触的初始步骤，该酶能够在或从其中的核酸分子中加入或除去化学部分 与一种或多种细胞或生物的活力相关的分子的存在。 由此产生新的可检测的核酸分子。 下一步涉及通过检测仅在与一种或多种细胞或生物体的活力相关的分子的存在下产生的新的核酸分子来检测与一种或多种细胞或生物体的存活力相关的分子的存在。 与活力相关的最优选的分子是ATP，尽管也可以检测到NAD。 用于该方法的优选酶是连接酶。 该方法具有许多应用，特别是在监测细胞的活力，毒理学测试和确定样品或表面是否存在污染。 还提供工具包来执行这些方法。

公开(公告)号：US07533795B2

公开(公告)日：2009-05-19

申请号：US10905255

申请日：2004-12-22

Applicant: Jon Conrad Schaeffer ,  Ariel Caesar-Prepena Jacala ,  Doyle C. Lewis ,  Thaddeus Jan Strusinski ,  Frederick Whitfield Dantzler, Jr. ,  Eugene Franklin Clemens ,  Paul Stuart Wilson ,  Micahel Butler ,  Jeffrey Aaron Killough

Inventor： Jon Conrad Schaeffer ,  Ariel Caesar-Prepena Jacala ,  Doyle C. Lewis ,  Thaddeus Jan Strusinski ,  Frederick Whitfield Dantzler, Jr. ,  Eugene Franklin Clemens ,  Paul Stuart Wilson ,  Micahel Butler ,  Jeffrey Aaron Killough

IPC: B23K31/02

CPC classification number: B23K31/02 ,  B23K35/304 ,  B23K2101/001 ,  B23K2103/26 ,  Y10T29/49318

Abstract: A weld process cuitable for repairing precipitation-strengthened superalloys, and particularly gamma prime-strengthened nickel-based superalloys. The process entails forming a weldment in a cavity present in a surface of an article formed of a precipitation-strengthened superalloy. The cavity has a root region and a cap region between the root region and the surface of the article. A solid body formed of a superalloy composition is placed in the root region of the cavity so as to occupy a first portion but not a second portion of the root region. A first filler material formed of a solid solution-strengthened superalloy is then weld-deposited in the second portion of the root region. Subsequently, a second filler material formed of a precipitation-strengthened superalloy is weld-deposited in the cap region of the cavity.

Abstract translation: 可用于修复沉淀强化超级合金，特别是γ初始强化的镍基超级合金的焊接工艺。 该方法需要在存在于由沉淀强化的超级合金形成的制品的表面中的空腔中形成焊件。 空腔具有在根部区域和物品表面之间的根部区域和帽区域。 由超合金组合物形成的固体被放置在空腔的根部区域中，以便占据根部区域的第一部分而不是第二部分。 然后将由固溶强化的超级合金形成的第一填料材料焊接沉积在根区的第二部分中。 接着，将由沉淀强化的高温合金形成的第二填料材料熔合沉积在空腔的盖区域中。

公开(公告)号：US20060183760A1

公开(公告)日：2006-08-17

申请号：US11238533

申请日：2005-09-28

Applicant: Peter Fischer ,  Michael Jarman ,  Edward McDonald ,  Bernard Nutley ,  Florence Raynaud ,  Stuart Wilson ,  Paul Workman

Inventor： Peter Fischer ,  Michael Jarman ,  Edward McDonald ,  Bernard Nutley ,  Florence Raynaud ,  Stuart Wilson ,  Paul Workman

IPC: A61K31/52 ,  C07D473/12

CPC classification number: C07D473/16

Abstract: The present invention relates to compounds of formula I or pharmaceutically acceptable salts thereof, wherein one of R1 and R2 is methyl, ethyl or isopropyl, and the other is H; R3 and R4 are each independently H, branched or un branched C1-C6 alkyl, or aryl, and wherein at least one of R3 and R4 is other than H; R5 is a branched or unbranched C1-C5 alkyl group or a C1-C6 cycloalkyl group, each of which may be optionally substituted with one or more OH groups; R6, R7, R8 and R9 are each independently H, halogen, NO2, OH, OMe, CN, NH2, COOH, CONH2, or SO2NH2. A further aspect of the invention relates to pharmaceutical compositions comprising compounds of formula 1, and the use of said compounds in treating proliferative disorders, viral disorders, CNS disorders, diabetes, stroke, alopecia or neurodegenerative disorders.

Abstract translation: 本发明涉及式I化合物或其药学上可接受的盐，其中R 1和R 2中的一个为甲基，乙基或异丙基，另一个为H; R 3和R 4各自独立地为H，支链或非支链C 1 -C 6烷基，或 芳基，并且其中R 3和R 4中的至少一个不是H; R 5是支链或非支链C 1 -C 5烷基或C 1 -C 3亚烷基 其中每一个可以任选地被一个或多个OH基团取代; R 6，R 7，R 8和R 9各自独立地为H，卤素，NO 3， 2，OH，OMe，CN，NH 2，COOH，CONH 2或SO 2 NH 2， / SUB>。 本发明的另一方面涉及包含式1化合物的药物组合物，以及所述化合物在治疗增殖性疾病，病毒性疾病，中枢神经系统疾病，糖尿病，中风，脱发或神经变性疾病中的用途。

公开(公告)号：US20060139210A1

公开(公告)日：2006-06-29

申请号：US11025397

申请日：2004-12-29

Applicant: Andy Stavros ,  Ming Tsai ,  Stuart Wilson

Inventor： Andy Stavros ,  Ming Tsai ,  Stuart Wilson

IPC: H01Q1/38

CPC classification number: H01Q9/285 ,  H01Q21/0025

Abstract: A phased antenna array is disclosed. The phased antenna array is composed of one or more modules and has a plurality of antenna. The array has a plurality of antenna configured to operate as an array and each module has at least one antenna. The modules have a substrate that supports the antenna, a microelectronic device for sending signals to or receiving signals from said antenna and conductive traces that connect that antenna to the microelectronic device. In those embodiments where the phased antenna array has more than one module, a common substrate supports the one or more modules. A combination of circuitry and interconnects achieves the desired electrical interconnection between the modules.

公开(公告)号：US20050283667A1

公开(公告)日：2005-12-22

申请号：US10710009

申请日：2004-06-11

Applicant: David Batten ,  Derek Eastwood ,  Stuart Wilson

Inventor： David Batten ,  Derek Eastwood ,  Stuart Wilson

IPC: G06F11/00

CPC classification number: G06F11/3672

Abstract: The invention includes an integrated toolset of software development aids for testing of software systems directed toward the production, access, management, and retrieval of stored data by providing a fast and efficient means for a developer to recreate production data within a testing environment. The toolset consists of components which, when combined, provide an interactive on-line system where a developer is provided with access to low volume, high-quality, depersonalized, and targeted data with referential integrity and is suitable for most testing needs.

Abstract translation: 本发明包括用于通过提供用于开发人员在测试环境中重新生成生产数据的快速且有效的手段来测试针对存储数据的生产，访问，管理和检索的软件系统的软件开发辅助的集成工具集。 该工具包由组件组成，当组合时，提供交互式在线系统，开发人员可以访问具有引用完整性的低容量，高质量，非人性化和有针对性的数据，并适合大多数测试需求。

公开(公告)号：US20050175988A1

公开(公告)日：2005-08-11

申请号：US10494896

申请日：2002-11-08

Applicant: Stuart Wilson ,  Christopher Stanley ,  Jay Patel

Inventor： Stuart Wilson ,  Christopher Stanley ,  Jay Patel

IPC: G01N33/542 ,  G01N33/58 ,  C12Q1/70

CPC classification number: G01N33/542 ,  G01N33/58

Abstract: A method of assay for the outcome of a reaction comprises conducting a reaction between a first reactant and a second reactant to produce from the first reactant a product having at least one new structural feature not present in the first reactant or the second reactant, e.g. by cleaving the first reactant or adding to it, producing a multiple-phage tagged complex comprising at least two different viruses and said product or said first reactant, in which complex at least one of said viruses is connected to said product using a said new structural feature or is lost from said first reactant upon formation of said new structural feature, and determining the presence or amount of said complex by a dual-phage assay.

Abstract translation: 测定反应结果的方法包括在第一反应物和第二反应物之间进行反应，以从第一反应物产生具有至少一个不存在于第一反应物或第二反应物中的新结构特征的产物。 通过切割第一反应物或加入其中，产生包含至少两种不同病毒和所述产物或所述第一反应物的多噬菌体标记复合物，其中至少一种所述病毒与所述产物连接，所述产物或所述第一反应物使用所述新结构 特征或在形成所述新结构特征时从所述第一反应物损失，并通过双噬菌体测定法确定所述复合物的存在或量。

公开(公告)号：US5992786A

公开(公告)日：1999-11-30

申请号：US117164

申请日：1998-12-17

Applicant: Martyn N. Palliser ,  Stuart Wilson

Inventor： Martyn N. Palliser ,  Stuart Wilson

IPC: B60R22/34

CPC classification number: B60R22/34

Abstract: A vehicle safety restraint retractor comprising a spool (2) for storage of belt webbing (1), the spool being mounted for rotation about its axis (3) within the spool housing (4) the retractor comprising a resilient biasing member (10) urging one end of the spool towards a corresponding cover for the side of the retractor, for taking up axial tolerances in the spool mounting. The resilient biasing member (10) is preferably a wave washer spring positioned at the spring side of the spool, between the spool and the spool arbour cap (9) and covered with a spring cover (7). Such a wave washer reduces axial and radial float of the spool and thus substantially reduces levels of noise in normal operation of the retractor.

Abstract translation: PCT No.PCT / GB97 / 00230 Sec。 371 1997年12月17日第 102（e）1998年12月17日日期PCT 1998年1月24日PCT PCT。 公开号WO97 / 27089 日期1997年7月31日一种车辆安全约束牵引器，包括用于存放皮带织带（1）的卷轴（2），所述卷轴安装成围绕其轴线（3）围绕卷轴壳体（4）旋转，牵引器包括弹性偏置 构件（10）将卷轴的一端朝向牵开器侧面的相应盖，用于卷取卷轴安装件中的轴向公差。 优选地，弹性偏置构件（10）是位于卷轴的弹簧侧的波形垫圈弹簧，位于卷轴和卷轴轴颈盖（9）之间并且被弹簧盖（7）覆盖。 这种波形垫圈减小了卷轴的轴向和径向浮动，因此在牵开器的正常操作中基本上降低了噪音水平。