公开(公告)号：US20110212438A1

公开(公告)日：2011-09-01

申请号：US13071108

申请日：2011-03-24

Applicant: Stuart Wilson ,  Sharon Banin

Inventor： Stuart Wilson ,  Sharon Banin

IPC: C12Q1/68 ,  C12M1/34

CPC classification number: C12Q1/25 ,  C12Q1/68 ,  C12Q1/6844 ,  C12Q2563/143 ,  C12Q2561/125 ,  C12Q2521/543

Abstract: Kits and devices for performing a method of detecting a molecule associated with viability of one or more cells or organisms in a sample are provided. The method comprises the initial step of contacting the sample with an enzyme, which enzyme is capable of adding or removing a chemical moiety to or from a nucleic acid molecule in the presence of the molecule associated with viability of the one or more cells or organisms. This thereby generates a novel detectable nucleic acid molecule. The next step involves detecting the presence of the molecule associated with viability of the one or more cells or organisms by detecting the novel nucleic acid molecule generated only in the presence of the molecule associated with viability of the one or more cells or organisms. A most preferred molecule associated with viability is ATP, although NAD may also be detected. A preferred enzyme for use in the methods is ligase. The kits and devices for performing the method have numerous applications, in particular in monitoring viability of cells, toxicology testing and determining whether there is contamination in a sample or on a surface.

Abstract translation: 提供了用于执行检测与样品中一种或多种细胞或生物体的活力相关的分子的方法的试剂盒和装置。 该方法包括使样品与酶接触的初始步骤，该酶能够在与一种或多种细胞或生物体的活力相关的分子存在下向核酸分子添加或除去化学部分。 由此产生新的可检测的核酸分子。 下一步涉及通过检测仅在与一种或多种细胞或生物体的活力相关的分子存在下产生的新的核酸分子来检测与一种或多种细胞或生物体的存活力相关的分子的存在。 与活力相关的最优选的分子是ATP，尽管也可以检测到NAD。 用于该方法的优选酶是连接酶。 用于执行该方法的试剂盒和装置具有许多应用，特别是在监测细胞的活力，毒理学测试和确定样品或表面是否存在污染。

公开(公告)号：US20060257879A1

公开(公告)日：2006-11-16

申请号：US10566792

申请日：2004-08-02

Applicant: Stuart Wilson ,  Christopher Stanley ,  Sharon Banin

Inventor： Stuart Wilson ,  Christopher Stanley ,  Sharon Banin

IPC: C12Q1/68 ,  C12Q1/34

CPC classification number: C12Q1/42 ,  C12Q1/6806 ,  G01N33/573 ,  G01N33/57434

Abstract: The present invention provides a method of detecting an enzyme in a sample wherein the enzyme is capable of adding or removing a chemical moiety to or from a nucleic acid molecule, which thereby confers altered sensitivity of the nucleic acid molecule in a subsequent process. The invention also relates to diagnostic methods which take advantage of the method of the invention and kits which are useful for carrying out the method of the invention.

Abstract translation: 本发明提供了一种检测样品中的酶的方法，其中酶能够向或从核酸分子添加或除去化学部分，从而赋予核酸分子在随后的方法中更高的敏感性。 本发明还涉及利用本发明方法和可用于实施本发明方法的试剂盒的诊断方法。

公开(公告)号：US08080375B2

公开(公告)日：2011-12-20

申请号：US11914697

申请日：2006-05-18

Applicant: Stuart Wilson ,  John Christopher Stanley ,  Sharon Banin

Inventor： Stuart Wilson ,  John Christopher Stanley ,  Sharon Banin

IPC: C12Q1/68

CPC classification number: G01N33/57434 ,  C12Q1/42 ,  C12Q1/682 ,  G01N33/573 ,  C12Q2563/179 ,  C12Q2521/525

Abstract: An improved method of detecting an enzyme in a sample, which enzyme is capable of adding or removing a chemical moiety to or from a nucleic acid molecule, thereby conferring the nucleic acid molecule with the ability to be extended to generate a novel detectable nucleic acid molecule, comprises the steps of allowing the sample to be tested for the presence of the enzyme to interact with the nucleic acid molecule; and testing for interaction of the enzyme with the nucleic acid molecule by detecting the novel nucleic acid molecule generated only in the presence of the enzyme. The preferred enzyme is a phosphatase. The methods have a number of applications, for example in enhancing the sensitivity of immunoassays, for detecting pathogen associated phosphatase, for diagnosing certain conditions and for detecting specific contaminants in a sample.

Abstract translation: 一种检测样品中酶的改进方法，该酶能够向或从核酸分子添加或除去化学部分，从而赋予具有延伸以产生新的可检测核酸分子的能力的核酸分子 包括以下步骤：使样品测试酶的存在与核酸分子相互作用; 并通过检测仅在酶存在下产生的新的核酸分子来测试酶与核酸分子的相互作用。 优选的酶是磷酸酶。 该方法具有许多应用，例如增强免疫测定的灵敏度，用于检测病原体相关磷酸酶，用于诊断某些条件并检测样品中的特定污染物。

公开(公告)号：US20090215047A1

公开(公告)日：2009-08-27

申请号：US11993986

申请日：2006-07-03

Applicant: Stuart Wilson ,  Sharon Banin

Inventor： Stuart Wilson ,  Sharon Banin

IPC: C12Q1/68

CPC classification number: C12Q1/25 ,  C12Q1/68 ,  C12Q1/6844 ,  Y02A50/52 ,  C12Q2563/143 ,  C12Q2561/125 ,  C12Q2521/543

Abstract: A method of detecting a molecule associated with viability of one or more cells or organisms in a sample comprises the initial step of contacting the sample with an enzyme, which enzyme is capable of adding or removing a chemical moiety to or from a nucleic acid molecule in the presence of the molecule associated with viability of the of the one or more cells or organisms. This thereby generates a novel detectable nucleic acid molecule. The next step involves detecting the presence of the molecule associated with viability of the one or more cells or organisms by detecting the novel nucleic acid molecule generated only in the presence of the molecule associated with viability of the one or more cells or organisms. A most preferred molecule associated with viability is ATP, although NAD may also be detected. A preferred enzyme for use in the methods is ligase. The method has numerous applications, in particular in monitoring viability of cells, toxicology testing and determining whether there is contamination in a sample or on a surface. Kits are also provided for carrying out the methods.

Abstract translation: 检测与样品中一种或多种细胞或生物体的活力相关的分子的方法包括使样品与酶接触的初始步骤，该酶能够在或从其中的核酸分子中加入或除去化学部分 与一种或多种细胞或生物的活力相关的分子的存在。 由此产生新的可检测的核酸分子。 下一步涉及通过检测仅在与一种或多种细胞或生物体的活力相关的分子的存在下产生的新的核酸分子来检测与一种或多种细胞或生物体的存活力相关的分子的存在。 与活力相关的最优选的分子是ATP，尽管也可以检测到NAD。 用于该方法的优选酶是连接酶。 该方法具有许多应用，特别是在监测细胞的活力，毒理学测试和确定样品或表面是否存在污染。 还提供工具包来执行这些方法。

公开(公告)号：US07943314B2

公开(公告)日：2011-05-17

申请号：US11993986

申请日：2006-07-03

Applicant: Stuart Wilson ,  Sharon Banin

Inventor： Stuart Wilson ,  Sharon Banin

IPC: C12Q1/68 ,  C07H21/02 ,  C07H21/04

CPC classification number: C12Q1/25 ,  C12Q1/68 ,  C12Q1/6844 ,  Y02A50/52 ,  C12Q2563/143 ,  C12Q2561/125 ,  C12Q2521/543

Abstract: A method of detecting a molecule associated with viability of one or more cells or organisms in a sample comprises the initial step of contacting the sample with an enzyme, which enzyme is capable of adding or removing a chemical moiety to or from a nucleic acid molecule in the presence of the molecule associated with viability of the of the one or more cells or organisms. This thereby generates a novel detectable nucleic acid molecule. The next step involves detecting the presence of the molecule associated with viability of the one or more cells or organisms by detecting the novel nucleic acid molecule generated only in the presence of the molecule associated with viability of the one or more cells or organisms. A most preferred molecule associated with viability is ATP, although NAD may also be detected. A preferred enzyme for use in the methods is ligase. The method has numerous applications, in particular in monitoring viability of cells, toxicology testing and determining whether there is contamination in a sample or on a surface. Kits are also provided for carrying out the methods.

Abstract translation: 检测与样品中一种或多种细胞或生物体的活力相关的分子的方法包括使样品与酶接触的初始步骤，该酶能够在或从其中的核酸分子中加入或除去化学部分 与一种或多种细胞或生物的活力相关的分子的存在。 由此产生新的可检测的核酸分子。 下一步涉及通过检测仅在与一种或多种细胞或生物体的活力相关的分子的存在下产生的新的核酸分子来检测与一种或多种细胞或生物体的存活力相关的分子的存在。 与活力相关的最优选的分子是ATP，尽管也可以检测到NAD。 用于该方法的优选酶是连接酶。 该方法具有许多应用，特别是在监测细胞的活力，毒理学测试和确定样品或表面是否存在污染。 还提供工具包来执行这些方法。

公开(公告)号：US20090098539A1

公开(公告)日：2009-04-16

申请号：US11914697

申请日：2006-05-18

Applicant: Stuart Wilson ,  John Christopher Stanley ,  Sharon Banin

Inventor： Stuart Wilson ,  John Christopher Stanley ,  Sharon Banin

IPC: C12Q1/68

CPC classification number: G01N33/57434 ,  C12Q1/42 ,  C12Q1/682 ,  G01N33/573 ,  C12Q2563/179 ,  C12Q2521/525

Abstract: An improved method of detecting an enzyme in a sample, which enzyme is capable of adding or removing a chemical moiety to or from a nucleic acid molecule, thereby conferring the nucleic acid molecule with the ability to be extended to generate a novel detectable nucleic acid molecule, comprises the steps of allowing the sample to be tested for the presence of the enzyme to interact with the nucleic acid molecule; and testing for interaction of the enzyme with the nucleic acid molecule by detecting the novel nucleic acid molecule generated only in the presence of the enzyme. The preferred enzyme is a phosphatase. The methods have a number of applications, for example in enhancing the sensitivity of immunoassays, for detecting pathogen associated phosphatase, for diagnosing certain conditions and for detecting specific contaminants in a sample.

Abstract translation: 一种检测样品中酶的改进方法，该酶能够向或从核酸分子添加或除去化学部分，从而赋予具有延伸以产生新的可检测核酸分子的能力的核酸分子 包括以下步骤：使样品测试酶的存在与核酸分子相互作用; 并通过检测仅在酶存在下产生的新的核酸分子来测试酶与核酸分子的相互作用。 优选的酶是磷酸酶。 该方法具有许多应用，例如增强免疫测定的灵敏度，用于检测病原体相关磷酸酶，用于诊断某些条件并检测样品中的特定污染物。

公开(公告)号：US20120107865A1

公开(公告)日：2012-05-03

申请号：US13264177

申请日：2010-04-19

Applicant: Stuart Wilson ,  William Mullen

Inventor： Stuart Wilson ,  William Mullen

IPC: C12Q1/25

CPC classification number: C12Q1/04 ,  C12Q1/68 ,  C12Q1/689 ,  C12Q1/6895 ,  C12Q2521/501 ,  C12Q2527/119 ,  C12Q2533/10 ,  C12Q2537/149 ,  G01N2333/9015

Abstract: A method of detecting a ligase expressing micro-organism in a sample comprises steps of treating the sample under conditions that inhibit the activity of ATP-dependent ligase from mammalian cells but which do not inhibit the activity of the microbial ligases, contacting the sample or a portion of the sample with a nucleic acid molecule which acts as a substrate for ligase activity in the sample, incubating the thus contacted sample under conditions suitable for ligase activity; and specifically determining the presence and/or the amount of a ligated nucleic acid molecule resulting from the action of the ligase on the substrate nucleic acid molecule to indicate the presence of the ligase expressing micro-organism. The micro-organism may be a fungus or a bacterium or both. High pH conditions may be employed to inactivate mammalian ligases. Related kits are described.

Abstract translation: 检测样品中连接酶表达微生物的方法包括以下步骤：在抑制ATP依赖性连接酶从哺乳动物细胞的活性但不抑制微生物连接酶的活性，接触样品或 样品的一部分与核酸分子起作用，作为样品中连接酶活性的底物，在适合连接酶活性的条件下孵育由此接触的样品; 并且具体地确定由连接酶对底物核酸分子的作用产生的连接的核酸分子的存在和/或量，以指示表达微生物的连接酶的存在。 微生物可以是真菌或细菌或两者。 可以使用高pH条件来灭活哺乳动物连接酶。 描述相关工具包。

公开(公告)号：US20100291564A1

公开(公告)日：2010-11-18

申请号：US12667818

申请日：2008-07-09

Applicant: Christopher John Stanley ,  Stuart Wilson

Inventor： Christopher John Stanley ,  Stuart Wilson

IPC: C12Q1/68 ,  C12N9/48

CPC classification number: C12Q1/04 ,  C12N9/93 ,  C12Q1/02 ,  C12Q1/18 ,  C12Q1/25 ,  C12Q1/689 ,  G01N2333/9015 ,  C12Q2565/301 ,  C12Q2521/501

Abstract: NAD-dependent ligase is identified as an indicator of micro-organisms in a sample. A method of detecting the presence of an NAD-dependent ligase expressing micro-organism in a sample comprises the steps of: (a) contacting the sample with a nucleic acid molecule which acts as a substrate for NAD-dependent ligase activity in the sample, (b) incubating the thus contacted sample under conditions suitable for NAD-dependent ligase activity; and (c) specifically determining the presence of a ligated nucleic acid molecule resulting from the action of the NAD-dependent ligase on the substrate nucleic acid molecule to indicate the presence of the NAD-dependent ligase expressing micro-organism. The method has a number of applications and kits for carrying out the methods are also provided. Lysostaphin preparations substantially free from nuclease and/or ligase contaminants are produced by heating a lysostaphin preparation which contains nuclease and/or ligase contaminants under conditions whereby nuclease and/or ligase activity is reduced whereas endopeptidase activity of the lysostaphin is substantially unaffected.

Abstract translation: NAD依赖性连接酶被鉴定为样品中微生物的指标。 检测样品中NAD依赖性连接酶表达微生物的存在的方法包括以下步骤：（a）使样品与作为样品中NAD依赖性连接酶活性的底物的核酸分子接触， （b）在适合于NAD依赖性连接酶活性的条件下孵育由此接触的样品; 和（c）特异性地确定由NAD依赖性连接酶在底物核酸分子上的作用产生的连接的核酸分子的存在，以指示表达微生物的NAD依赖性连接酶的存在。 该方法还提供了许多用于执行方法的应用和套件。 通过在其中核酸酶和/或连接酶活性降低的条件下加热含有核酸酶和/或连接酶污染物的溶葡萄球菌素制剂而产生基本上不含核酸酶和/或连接酶污染物的溶葡萄球菌素制剂，而溶葡萄球菌素的内肽酶活性基本上不受影响。

公开(公告)号：US20050221404A1

公开(公告)日：2005-10-06

申请号：US10506131

申请日：2003-02-28

Applicant: Amin Lane ,  Christopher Stanley ,  Stuart Wilson

Inventor： Amin Lane ,  Christopher Stanley ,  Stuart Wilson

IPC: G01N33/68 ,  C07K1/22 ,  C07K14/47 ,  G01N33/53 ,  G01N33/537 ,  G01N33/543

CPC classification number: G01N33/6803 ,  G01N33/54326 ,  G01N33/68 ,  G01N33/6896 ,  G01N2333/968 ,  G01N2800/2828

Abstract: Infective aggregating forms of proteins such as PrP, amyloid, and tau are bound selectively in the presence of the normal form protein using a polyionic binding agent such as dextran sulphate or pentosan (anionic), or polyamine compounds such as pDADMAC (cationic) under selective binding conditions including the use of n-lauroylsarcosine at mildly alkaline pH, and may then be assayed.

Abstract translation: 蛋白质如PrP，淀粉样蛋白和tau的感染性聚集形式在正常形式的蛋白质存在下使用聚离子结合剂如硫酸葡聚糖或戊聚糖（阴离子）或多胺化合物如pDADMAC（阳离子）在选择性 包括在弱碱性pH下使用正月桂酰肌氨酸的结合条件，然后可以进行测定。

公开(公告)号：US20050009846A1

公开(公告)日：2005-01-13

申请号：US10742237

申请日：2003-12-18

Applicant: Peter Fischer ,  Mike Jarman ,  Ted McDonald ,  Bernard Nutley ,  Florence Raynaud ,  Stuart Wilson ,  Paul Workman

Inventor： Peter Fischer ,  Mike Jarman ,  Ted McDonald ,  Bernard Nutley ,  Florence Raynaud ,  Stuart Wilson ,  Paul Workman

IPC: A61K31/52 ,  A61P17/06 ,  A61P25/28 ,  A61P31/12 ,  A61P35/00 ,  A61P35/02 ,  A61P43/00 ,  C07D473/16 ,  C07D473/14

CPC classification number: C07D473/16

Abstract: The present invention relates to compounds of formula I or a pharmaceutically acceptable salt thereof wherein R2 is 2-hydroxymethylpyrrolidin-1-yl, or NHCH(R4)CH(R3)OH, wherein R3 is hydrogen or methyl and R4 is methyl, ethyl or isopropyl; R6 is 3-nitrophenylamino, 3,4-dimethoxybenzylamino, 3-iodobenzyl-amino, pyrid-2-yl-methylamino, pyrid-4-yl-methylamino or indan-5-amino; R9 is isopropyl or cyclopentanyl. In a further aspect, the invention relates to pharmaceutical compositions comprising said compounds, and the use thereof in treating antiproliferative disorders and or viral disorders.

Abstract translation: 本发明涉及式I化合物或其药学上可接受的盐，其中R2是2-羟甲基吡咯烷-1-基或NHCH（R4）CH（R3）OH，其中R3是氢或甲基，R4是甲基，乙基或 异丙基 R6是3-硝基苯基氨基，3,4-二甲氧基苄基氨基，3-碘苄基 - 氨基，吡啶-2-基 - 甲基氨基，吡啶-4-基 - 甲基氨基或茚满-5-氨基; R9是异丙基或环戊基。 在另一方面，本发明涉及包含所述化合物的药物组合物及其在治疗抗增生性疾病和/或病毒性疾病中的用途。