公开(公告)号：US20180217140A1

公开(公告)日：2018-08-02

申请号：US15877658

申请日：2018-01-23

Applicant: Bio-Rad Laboratories, Inc.

Inventor： William Strong ,  Clayton T. McKee

IPC: G01N33/558 ,  G01N33/543 ,  B01L3/00

CPC classification number: G01N33/558 ,  B01L3/5023 ,  B01L2200/0689 ,  B01L2300/069 ,  B01L2300/165 ,  B01L2400/0406 ,  B01L2400/0475 ,  G01N33/54386

Abstract: Lateral flow devices, methods and kits for performing lateral flow assays are provided.

公开(公告)号：US09895693B2

公开(公告)日：2018-02-20

申请号：US14642487

申请日：2015-03-09

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Clayton T. McKee ,  William Strong

IPC: G01N33/543 ,  B01L3/00 ,  G01N27/447 ,  C12Q1/68

CPC classification number: B01L3/502738 ,  B01L2200/0605 ,  B01L2200/0642 ,  B01L2300/045 ,  B01L2300/047 ,  B01L2300/0816 ,  B01L2300/0874 ,  B01L2400/0415 ,  B01L2400/0418 ,  B01L2400/0421 ,  B01L2400/0487 ,  B01L2400/065 ,  C12Q1/6806 ,  G01N27/44717 ,  G01N27/44739 ,  G01N33/54366

Abstract: Devices, systems, methods, and kits are provided for performing separation, immobilization, blotting, and/or detection of analytes from biological samples. In some embodiments, the devices are constructed from two solid substrates with surfaces in contact. The devices include a plurality of channels formed from indentations in these surfaces. The indentations can be aligned with each other across the interface between the substrates, and realigned by shifting or sliding one substrate relative to the other. In some embodiments, the devices are constructed from three layers of a solid substrate. A separation channel in the middle layer of the device is first used for analyte separation. The middle layer can then be slid relative the top and/or bottom layer, thereby aligning the separation channel with a blotting membrane. Analytes can then be transferred to the membrane using electrodes in the top and bottom layers.

公开(公告)号：US20170191997A1

公开(公告)日：2017-07-06

申请号：US15388778

申请日：2016-12-22

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Clayton T. McKee ,  William Strong ,  Mingde Zhu ,  Emmanuel Quiroz

IPC: G01N33/558 ,  G01N33/543

CPC classification number: G01N33/558 ,  G01N33/54386

Abstract: A lateral flow apparatus is provided.

公开(公告)号：US09594054B2

公开(公告)日：2017-03-14

申请号：US13950590

申请日：2013-07-25

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Clayton T. McKee ,  William Strong

IPC: G01N27/447

CPC classification number: G01N27/44739 ,  G01N27/44747 ,  G01N33/54306 ,  G01N33/54393

Abstract: Molecular species that are immobilized in discrete locations on a planar support such as protein bands on a gel or a blotting membrane or species applied in dots or spots on a membrane are reacted with binding reagents that are applied through a porous hydrophilic transfer sheet placed over the planar support, the sheet having at least one region that is laterally bordered by a barrier with the binding reagent retained within the bordered region. The bordered region is placed directly over an area on the planar support where the molecular species are expected to reside if they are present on the support. The binding reagent is then delivered into the support to contact the species. Targeted delivery of the binding reagent is thus achieved with improved efficiency.

Abstract translation: 固定在平面载体上的离散位置上的分子物质如凝胶或印迹膜上的蛋白质条带或涂在膜上的点或点上的物质与通过多孔亲水转移片施加的结合试剂反应， 所述片材具有至少一个区域，所述至少一个区域被阻挡物侧向界定，所述粘合剂保留在所述边界区域内。 如果分子种类存在于支持体上，则边界区域直接放置在平面支撑体上的分子种类预期驻留的区域上。 然后将结合试剂输送到载体中以接触物种。 因此，以提高的效率实现了结合试剂的靶向递送。

公开(公告)号：US20130231462A1

公开(公告)日：2013-09-05

申请号：US13776403

申请日：2013-02-25

Applicant: BIO-RAD LABORATORIES, INC.

Inventor： Steve Roth ,  William Strong

IPC: C07K16/42

CPC classification number: C07K16/42 ,  C07K2317/31 ,  C07K2317/32

Abstract: Provided are methods and compositions for immunoassays with improved sensitivity and specificity. The presently disclosed anti-immune complex antibodies stabilize the interaction between a primary and secondary antibody, thereby allowing for more stringent wash conditions, less background, and stronger signal.

Abstract translation: 提供了具有改善的灵敏度和特异性的免疫测定法的方法和组合物。 目前公开的抗免疫复合物抗体稳定了一级和二级抗体之间的相互作用，从而允许更严格的洗涤条件，更少的背景和更强的信号。