公开(公告)号：US12128415B2

公开(公告)日：2024-10-29

申请号：US17195206

申请日：2021-03-08

Applicant: Pattern Bioscience, Inc.

Inventor： Ross Johnson ,  Jonathan Isom ,  David Bussian ,  Nicolas Arab

IPC: B01L9/00 ,  B01L1/02 ,  B01L3/00 ,  G01N21/31

CPC classification number: B01L9/527 ,  B01L1/02 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502784 ,  G01N21/31 ,  B01L2200/0642 ,  B01L2200/0647 ,  B01L2300/0663 ,  B01L2400/049

Abstract: An apparatus for loading and imaging a microfluidic chip can comprise a housing having walls that define a vacuum chamber and a first receptacle disposed within the vacuum chamber, the first receptacle defining a space for receiving one or more microfluidic chips. The apparatus can also include a negative pressure source, a light source, and an optical sensor coupled to the housing. The negative pressure source can be configured to reduce pressure within the vacuum chamber, the light source can be positioned to illuminate at least a portion of the space for receiving the chip(s), and the optical sensor can be positioned to capture an image of at least a portion of the space for receiving the chip(s).

公开(公告)号：US20240342712A1

公开(公告)日：2024-10-17

申请号：US18624324

申请日：2024-04-02

Applicant: BACTEROMIC SP. Z O.O.

Inventor： Piotr GARSTECKI ,  Pawel DEBSKI ,  Jaroslaw ZIÓLKOWSKI ,  Piotr KNAP

IPC: B01L3/00

CPC classification number: B01L3/50273 ,  B01L2200/0642 ,  B01L2200/0673 ,  B01L2300/0864 ,  B01L2400/0677

Abstract: A microfluidic chip for conducting microbiological assays, includes a substrate in which incubation segments, a sample reservoir and microfluidic channels connecting the sample reservoir with the incubation segments are arranged. The microfluidic chip further includes a non-aqueous liquid reservoir for containing non-aqueous liquid wherein the reservoir is connectable via a releasable airtight and liquid-tight valve with the microfluidic channels connecting the sample reservoir with the incubation segments each incubation segment comprises an incubation well connected by a gas-exchange channel to an unvented gas cavity.

公开(公告)号：US20240307876A1

公开(公告)日：2024-09-19

申请号：US18674548

申请日：2024-05-24

Applicant: STMICROELECTRONICS S.r.l.

Inventor： Marco CEREDA ,  Lillo RAIA ,  Danilo PIROLA

IPC: B01L3/00 ,  A61B5/15 ,  A61B5/154 ,  B01F33/30 ,  B01F33/40 ,  B01F33/452 ,  B01L9/00 ,  C12M3/06 ,  C12Q1/6806 ,  C12Q1/6844

CPC classification number: B01L3/502753 ,  A61B5/15003 ,  A61B5/150221 ,  A61B5/150351 ,  A61B5/154 ,  B01F33/30 ,  B01F33/406 ,  B01F33/452 ,  B01L3/502715 ,  B01L3/502723 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/5029 ,  B01L3/508 ,  B01L3/5082 ,  B01L3/52 ,  B01L3/527 ,  B01L9/527 ,  C12M23/16 ,  A61B5/150389 ,  A61B5/150503 ,  A61B5/150732 ,  B01L3/5023 ,  B01L3/502707 ,  B01L3/50825 ,  B01L3/523 ,  B01L2200/025 ,  B01L2200/026 ,  B01L2200/0621 ,  B01L2200/0642 ,  B01L2200/0668 ,  B01L2200/0673 ,  B01L2200/0689 ,  B01L2200/10 ,  B01L2200/141 ,  B01L2200/16 ,  B01L2300/022 ,  B01L2300/023 ,  B01L2300/044 ,  B01L2300/0609 ,  B01L2300/0681 ,  B01L2300/08 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0829 ,  B01L2300/0848 ,  B01L2300/0851 ,  B01L2300/0858 ,  B01L2300/0864 ,  B01L2300/087 ,  B01L2300/12 ,  B01L2300/16 ,  B01L2300/1827 ,  B01L2400/04 ,  B01L2400/0406 ,  B01L2400/043 ,  B01L2400/0487 ,  B01L2400/049 ,  B01L2400/0677 ,  C12Q1/6806 ,  C12Q1/6844

Abstract: An analysis unit formed by an analysis body housing an analysis chamber and having a sample inlet and a supply channel configured to fluidically connect the sample inlet to the analysis chamber. Dried assay reagents are arranged in the analysis chamber and are contained in an alveolar mass. For instance, the alveolar mass is a lyophilized mass formed by excipients and by assay-specific reagents.

公开(公告)号：US20240299944A1

公开(公告)日：2024-09-12

申请号：US18664102

申请日：2024-05-14

Applicant: Illumina, Inc.

Inventor： Arash Jamshidi ,  Yan-you Lin ,  Farnaz Absalan ,  Sarah Stuart ,  Gordon Cann ,  Yir-Shyuan Wu ,  Tarun Khurana ,  Jeffrey S Fisher

IPC: B01L3/00 ,  C12Q1/6806 ,  C12Q1/6869 ,  G01N1/28 ,  G01N15/10 ,  G01N33/543

CPC classification number: B01L3/502761 ,  B01L3/502784 ,  B01L3/5088 ,  C12Q1/6806 ,  G01N1/28 ,  G01N33/54333 ,  B01L2200/0605 ,  B01L2200/0642 ,  B01L2200/0668 ,  B01L2300/0819 ,  B01L2300/165 ,  B01L2400/0427 ,  B01L2400/043 ,  C12Q1/6869 ,  C12Q2563/143 ,  C12Q2563/149 ,  C12Q2563/185 ,  G01N2015/1006

Abstract: In accordance with embodiments herein a method for capturing cells of interest in a digital microfluidic system is provided, comprising utilizing a droplet actuator to transport a sample droplet to a microwell device. The microwell device includes a substrate having a plurality of microwells that open onto a droplet operations surface of the microwell device. The sample droplet includes cells of interest that enter the microwells. The method introduces capture beads to the microwells, and the capture elements are immobilized on the capture beads. The method utilizes the droplet actuator to transport a cell lysis reagent droplet to the microwell device. Portions of the cell lysis reagent droplet enter the microwells and, during an incubation period, cause the cells of interest to release analyte that is captured by the capture elements on the capture beads.

公开(公告)号：US20240210434A1

公开(公告)日：2024-06-27

申请号：US18421547

申请日：2024-01-24

Applicant: SiPhox, Inc.

Inventor： Michael Zalmon Dubrovsky ,  Azra Horowitz ,  Nithin Parsan ,  Diedrik Rene Vermeulen

IPC: G01N35/10 ,  B01L3/00 ,  G01N35/00

CPC classification number: G01N35/1074 ,  B01L3/502715 ,  B01L2200/04 ,  B01L2200/0642 ,  B01L2300/0654 ,  G01N2035/00524 ,  G01N2035/1032

Abstract: Disclosed herein are automated biosensing devices for biosensing including a set of sensor units organized in an array. Each of the sensor units includes an optical reader optically coupled to a corresponding sensor photonic integrated circuit (PIC). Each sensor PIC includes one or more optical analyte sensors each functionalized by a respective layer of binding ligands. Each optical reader is configured to provide optical signals to the one or more optical analyte sensors of the corresponding sensor PIC. An automated biosensing device can further include a robotic dipping head configured to dip each of the sensor PICs into a respective test sample.

公开(公告)号：US12017223B2

公开(公告)日：2024-06-25

申请号：US17154479

申请日：2021-01-21

Applicant: Sharp Life Science (EU) Limited

Inventor： Emma Jayne Walton ,  Lesley Anne Parry-Jones ,  Julie Karen Deacon

IPC: B01L3/00

CPC classification number: B01L3/502792 ,  B01L3/502715 ,  B01L3/502723 ,  B01L2200/027 ,  B01L2200/0605 ,  B01L2200/0642 ,  B01L2200/0673 ,  B01L2200/0684 ,  B01L2300/0809 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/165 ,  B01L2400/0406 ,  B01L2400/0427

Abstract: A microfluidic AM-EWOD device and a method of filling such a device are provided. The device comprises a chamber having one or more inlet ports. The device is configured, when the chamber contains a metered volume of a filler fluid that partially fills the chamber, preferentially maintain the metered volume of the filler fluid in a part of the chamber. The device is configured to allow displacement of some of the filler fluid from the part of the chamber when a volume of an assay fluid introduced into one of the one or more inlet ports enters the part of the chamber, thereby causing a volume of a venting fluid to vent from the chamber.

公开(公告)号：US11998917B2

公开(公告)日：2024-06-04

申请号：US17167228

申请日：2021-02-04

Applicant: Sharp Life Science (EU) Limited

Inventor： Emma Jayne Walton ,  Lesley Anne Parry-Jones ,  Julie Karen Deacon

IPC: B01L3/00

CPC classification number: B01L3/502792 ,  B01L3/502715 ,  B01L3/502723 ,  B01L2200/027 ,  B01L2200/0605 ,  B01L2200/0642 ,  B01L2200/0673 ,  B01L2200/0684 ,  B01L2300/0809 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/165 ,  B01L2400/0406 ,  B01L2400/0427

Abstract: A microfluidic AM-EWOD device and a method of filling such a device are provided. The device comprises a chamber having one or more inlet ports. The device is configured, when the chamber contains a metered volume of a filler fluid that partially fills the chamber, preferentially maintain the metered volume of the filler fluid in a part of the chamber. The device is configured to allow displacement of some of the filler fluid from the part of the chamber when a volume of an assay fluid introduced into one of the one or more inlet ports enters the part of the chamber, thereby causing a volume of a venting fluid to vent from the chamber.

公开(公告)号：US11931735B2

公开(公告)日：2024-03-19

申请号：US16970920

申请日：2019-02-05

Applicant: Oxford University Innovation Limited

Inventor： Edmond Walsh ,  Alexander Feuerborn ,  Peter Richard Cook ,  Cristian Soitu

IPC: B01J19/00 ,  B01L3/00 ,  B01L3/02 ,  C12M1/32 ,  C12M3/06

CPC classification number: B01L3/502707 ,  B01J19/0046 ,  B01L3/0293 ,  B01L3/502769 ,  B01L3/5088 ,  C12M23/12 ,  C12M23/16 ,  B01J2219/00531 ,  B01J2219/00617 ,  B01J2219/00621 ,  B01J2219/00659 ,  B01J2219/00743 ,  B01L2200/0642 ,  B01L2200/12 ,  B01L2300/0816 ,  B01L2300/161 ,  B01L2400/0442

Abstract: Methods and apparatus for manufacturing a microfluidic arrangement are disclosed. In one arrangement, a continuous body of a first liquid is provided in direct contact with a first substrate. A second liquid covers the first liquid. A separation fluid, immiscible with the first liquid, is propelled through at least the first liquid and into contact with the first substrate along all of a selected path on the surface of the first substrate. First liquid that was initially in contact with all of the selected path is displaced away from the selected path. The first liquid is divided to form sub-bodies of first liquid that are separated from each other. For each of one or more of the sub-bodies, a sub-body footprint represents an area of contact between the sub-body and the first substrate, and all of a boundary of the sub-body footprint is in contact with a closed loop of the selected path surrounding the sub-body footprint.

公开(公告)号：US20230323270A1

公开(公告)日：2023-10-12

申请号：US18318483

申请日：2023-05-16

Applicant: CURIOX BIOSYSTEMS PTE LTD.

Inventor： Kong Leong CHENG ,  Namyong Kim

IPC: C12M1/26 ,  B01L3/02 ,  C12M1/00 ,  G01N35/10

CPC classification number: C12M33/04 ,  B01L13/02 ,  B01L3/0293 ,  C12M29/00 ,  G01N35/1016 ,  B01L2200/0605 ,  B01L2200/0642 ,  B01L2300/0832 ,  B01L2300/165 ,  G01N2035/00524

Abstract: A method for washing particles includes obtaining an array plate that includes an array of hydrophilic areas surrounded by one or more hydrophobic areas. A respective solution containing a sample is located on a respective hydrophilic area of the array of hydrophilic areas. The respective hydrophilic area includes one or more indentations from a respective surrounding hydrophobic area of the one or more hydrophobic areas. The respective hydrophilic area includes a first indented surface that is offset from a reference surface defined by the respective surrounding hydrophobic area. The method also includes placing an aspirator nozzle above the respective hydrophilic area at a predefined distance from the first indented surface, and aspirating the solution with the aspirator nozzle while the aspirator nozzle is located at the predefined distance from the first indented surface.

公开(公告)号：US11667970B2

公开(公告)日：2023-06-06

申请号：US16939665

申请日：2020-07-27

Applicant: The Board of Trustees of the University of Illinois ,  Mayo Foundation for Medical Education and Research

Inventor： Rashid Bashir ,  Anurup Ganguli ,  Farhad Kosari

IPC: C12Q1/68 ,  C12Q1/6874 ,  C12N15/10 ,  B01L3/00 ,  C12Q1/686 ,  G16B25/00 ,  G01N1/28 ,  G16B25/10 ,  G16B30/00 ,  C12Q1/6827

CPC classification number: C12Q1/6874 ,  B01L3/5027 ,  B01L3/5085 ,  C12N15/1003 ,  C12N15/1096 ,  C12Q1/686 ,  G01N1/286 ,  G16B25/00 ,  G16B25/10 ,  B01L3/50851 ,  B01L2200/0642 ,  B01L2300/0819 ,  B01L2300/0858 ,  B01L2300/0893 ,  C12Q1/6827 ,  G01N2001/282 ,  G01N2001/2873 ,  G16B30/00 ,  C12N15/1003 ,  C12Q2521/107

Abstract: Various methods and devices for spatial molecular analysis from tissue is provided. For example, a method of spatially mapping a tissue sample is provided with a microarray having a plurality of wells, wherein adjacent wells are separated by a shearing surface; overlaying said microarray with a tissue sample; applying a deformable substrate to an upper surface of said tissue sample; applying a force to the deformable substrate, thereby forcing underlying tissue sample into the plurality of wells; shearing the tissue sample along the shearing surface into a plurality of tissue sample islands, with each unique tissue sample island positioned in a unique well; and imaging or quantifying said plurality of tissue sample islands, thereby generating a spatial map of said tissue sample. The imaging and/or quantifying may use a nucleic acid amplification technique.