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57 条记录 (耗时 0.062 秒)

    BINARY PROBE AND CLAMP COMPOSITION AND METHODS FOR TARGET HYBRIDIZATION DETECTION
    32.
    发明申请
    BINARY PROBE AND CLAMP COMPOSITION AND METHODS FOR TARGET HYBRIDIZATION DETECTION 有权
    二次探针和夹钳组合物和方法进行目标杂交检测

    公开(公告)号：US20100291557A1

    公开(公告)日：2010-11-18

    申请号：US12538823

    申请日：2009-08-10

    申请人： Kenneth J. Livak ,  Michael Egholm ,  Michael W. Hunkapiller

    发明人： Kenneth J. Livak ,  Michael Egholm ,  Michael W. Hunkapiller

    IPC分类号： C12Q1/68 ,  C07H21/04

    CPC分类号： C12Q1/6816 ,  C12Q1/6818 ,  C12Q2549/126 ,  C12Q2537/119 ,  C12Q2525/179

    摘要： Binary probe and clamp compositions conduct methods for target hybridization detection. Where the probe is a substrate for exonuclease cleavage, the composition provides quantitation and detection of PCR products, by real-time and end-point measurements. Where the probe is an amplification primer, the composition provides an improved method for labelling and detection of PCR products. Probes and clamps may be labelled with fluorescent dyes, quenchers, hybridization-stabilizing moieties, chemiluminescent dyes, and affinity ligands. Clamps may be nucleic acid analogs, such as 2-aminoethylglycine PNA.

    摘要翻译： 二进制探针和夹具组合物进行靶杂交检测的方法。 当探针是外切核酸酶切割的底物时，组合物通过实时和终点测量提供PCR产物的定量和检测。 当探针是扩增引物时，组合物提供了PCR产物的标记和检测的改进方法。 探针和夹具可以用荧光染料，猝灭剂，杂交稳定部分，化学发光染料和亲和配体标记。 夹具可以是核酸类似物，例如2-氨基乙基甘氨酸PNA。

    Binary probe and clamp composition
    34.
    发明授权
    Binary probe and clamp composition 有权
    二进制探针和夹具组成

    公开(公告)号：US07057025B2

    公开(公告)日：2006-06-06

    申请号：US10112677

    申请日：2002-03-28

    申请人： Kenneth J. Livak ,  Michael Egholm ,  Michael W. Hunkapiller

    发明人： Kenneth J. Livak ,  Michael Egholm ,  Michael W. Hunkapiller

    IPC分类号： C07H21/02 ,  C07H21/04 ,  C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/6816 ,  C12Q1/6818 ,  C12Q2549/126 ,  C12Q2537/119 ,  C12Q2525/179

    摘要： Binary probe and clamp compositions conduct methods for target hybridization detection. Where the probe is a substrate for exonuclease cleavage, the composition provides quantitation and detection of PCR products, by real-time and end-point measurements. Where the probe is an amplification primer, the composition provides an improved method for labelling and detection of PCR products. Probes and clamps may be labelled with fluorescent dyes, quenchers, hybridization-stabilizing moieties, chemiluminescent dyes, and affinity ligands. Clamps may be nucleic acid analogs, such as 2-aminoethylglycine PNA.

    摘要翻译： 二进制探针和夹具组合物进行靶杂交检测的方法。 当探针是外切核酸酶切割的底物时，组合物通过实时和终点测量提供PCR产物的定量和检测。 当探针是扩增引物时，组合物提供了PCR产物的标记和检测的改进方法。 探针和夹具可以用荧光染料，猝灭剂，杂交稳定部分，化学发光染料和亲和配体标记。 夹具可以是核酸类似物，例如2-氨基乙基甘氨酸PNA。

    BINARY PROBE AND CLAMP COMPOSITION.
    36.
    发明申请
    BINARY PROBE AND CLAMP COMPOSITION. 有权
    二进制和夹子组成。

    公开(公告)号：US20060035217A1

    公开(公告)日：2006-02-16

    申请号：US10112677

    申请日：2002-03-28

    申请人： Kenneth Livak ,  Michael Egholm ,  Michael Hunkapiller

    发明人： Kenneth Livak ,  Michael Egholm ,  Michael Hunkapiller

    IPC分类号： C12Q1/68 ,  C07H21/04

    CPC分类号： C12Q1/6816 ,  C12Q1/6818 ,  C12Q2549/126 ,  C12Q2537/119 ,  C12Q2525/179

    摘要： Binary probe and clamp compositions conduct methods for target hybridization detection. Where the probe is a substrate for exonuclease cleavage, the composition provides quantitation and detection of PCR products, by real-time and end-point measurements. Where the probe is an amplification primer, the composition provides an improved method for labelling and detection of PCR products. Probes and clamps may be labelled with fluorescent dyes, quenchers, hybridization-stabilizing moieties, chemiluminescent dyes, and affinity ligands. Clamps may be nucleic acid analogs, such as 2-aminoethylglycine PNA.

    摘要翻译： 二进制探针和夹具组合物进行靶杂交检测的方法。 当探针是外切核酸酶切割的底物时，组合物通过实时和终点测量提供PCR产物的定量和检测。 当探针是扩增引物时，组合物提供了PCR产物的标记和检测的改进方法。 探针和夹具可以用荧光染料，猝灭剂，杂交稳定部分，化学发光染料和亲和配体标记。 夹具可以是核酸类似物，例如2-氨基乙基甘氨酸PNA。

    Asynchronous primed PCR
    37.
    发明授权
    Asynchronous primed PCR 有权

    公开(公告)号：US06887664B2

    公开(公告)日：2005-05-03

    申请号：US09875211

    申请日：2001-06-05

    申请人： Caifu Chen ,  Michael Egholm ,  Lawrence A. Haff

    发明人： Caifu Chen ,  Michael Egholm ,  Lawrence A. Haff

    IPC分类号： G01N33/53 ,  C12N15/09 ,  C12Q1/68 ,  G01N33/566 ,  C07H21/00 ,  C07H21/02 ,  C12P19/34

    CPC分类号： C12Q1/686 ,  C12Q2561/101 ,  C12Q2527/113 ,  C12Q2527/107

    摘要： An asynchronous thermal cycling protocol for nucleic acid amplification uses two primers with thermal melting temperatures different by about 10 to 30° C. After the higher melting primer has annealed and polymerase mediated extension, the uncopied, single-stranded target sequence may be hybridized and detected by a probe. DNA probes may be cleaved by the exonuclease activity of a polymerase. The probe may be a non-cleaving analog such as PNA. When a probe is labelled with a reporter dye and a quencher selected to undergo energy transfer, e.g. FRET, fluorescence from the reporter dye may be effectively quenched when the probe is unbound. Upon hybridization of the probe to complementary target or upon cleavage while bound to target, the reporter dye is no longer quenched, resulting in a detectable amount of fluorescence. The second, lower-melting primer may be annealed and extended to generate a double-stranded nucleic acid. Amplification may be monitored in real time, including each cycle, or at the end point. The asynchronous PCR thermal cycling protocol can generate a preponderance of the PCR amplicon in single-stranded form by repetition at the end of the protocol of annealing and extension of the higher melting primer.

    Binary probe and clamp composition and methods for a target hybridization detection
    38.
    发明授权
    Binary probe and clamp composition and methods for a target hybridization detection 有权
    用于靶杂交检测的二进制探针和夹钳组成及方法

    公开(公告)号：US06432642B1

    公开(公告)日：2002-08-13

    申请号：US09232000

    申请日：1999-01-15

    申请人： Kenneth J. Livak ,  Michael Egholm ,  Michael W. Hunkapiller

    发明人： Kenneth J. Livak ,  Michael Egholm ,  Michael W. Hunkapiller

    IPC分类号： C12Q168

    CPC分类号： C12Q1/6816 ,  C12Q1/6818 ,  C12Q2549/126 ,  C12Q2537/119 ,  C12Q2525/179

    摘要： Binary probe and clamp compositions conduct methods for target hybridization detection. Where the probe is a substrate for exonuclease cleavage, the composition provides quantitation and detection of PCR products, by real-time and end-point measurements. Where the probe is an amplification primer, the composition provides an improved method for labelling and detection of PCR products. Probes and clamps may be labelled with fluorescent dyes, quenchers, hybridization-stabilizing moieties, chemiluminescent dyes, and affinity ligands. Clamps may be nucleic acid analogs, such as 2-aminoethylglycine PNA.

    摘要翻译： 二进制探针和夹具组合物进行靶杂交检测的方法。 当探针是外切核酸酶切割的底物时，组合物通过实时和终点测量提供PCR产物的定量和检测。 当探针是扩增引物时，组合物提供了PCR产物的标记和检测的改进方法。 探针和夹具可以用荧光染料，猝灭剂，杂交稳定部分，化学发光染料和亲和配体标记。 夹具可以是核酸类似物，例如2-氨基乙基甘氨酸PNA。

    Use of nucleic acid analogues in diagnostics and analytical procedures
    39.
    发明授权
    Use of nucleic acid analogues in diagnostics and analytical procedures 失效
    在诊断和分析程序中使用核酸类似物

    公开(公告)号：US06357163B1

    公开(公告)日：2002-03-19

    申请号：US08150156

    申请日：1994-05-04

    申请人： Ole Buchardt ,  Michael Egholm ,  Peter E. Nielsen ,  Rolf H. Berg

    发明人： Ole Buchardt ,  Michael Egholm ,  Peter E. Nielsen ,  Rolf H. Berg

    IPC分类号： C12Q168

    CPC分类号： C07H21/00 ,  A61K38/00 ,  C07K5/06026 ,  C07K5/06139 ,  C07K7/06 ,  C07K7/08 ,  C07K14/003 ,  C12Q1/68 ,  C12Q1/6813 ,  C12Q1/6832 ,  C12Q1/6869 ,  C12Q2535/107 ,  C12Q2525/107

    摘要： Methods of capture, recognition, detection, identification or quantitation of nucleic acids and diagnostics uses generally are described in which are used: (a) a peptide nucleic acid (PNA) comprising a polyamide backbone bearing a plurality of ligands at respective spaced locations along said backbone, said ligands being each independently naturally occurring nucleobases, non-naturally occurring nucleobases or nucleobase-binding groups, each said ligand being bound directly or indirectly to a nitrogen atom in said backbone, and said ligand bearing nitrogen atoms mainly being separated from one another in said backbone by from 4 to 8 intervening atoms; or (b) a nucleic acid analogue capable of hybridizing to a nucleic acid of complementary sequence to form a hybrid which is more stable against denaturation by heat than a hybrid between the conventional deoxyribonucleotide corresponding to said analogue and said nucleic acid; or (c) a nucleic acid analogue capable of hybridizing to a double stranded nucleic acid in which one strand has a sequence complementary to said analogue, so as to displace the other strand from said one strand.

    摘要翻译： 通常描述了核酸和诊断用途的捕获，识别，检测，鉴定或定量的方法，其中使用：（a）肽核酸（PNA），其包含聚酰胺主链，所述聚酰胺主链在所述 所述配体各自独立地是天然存在的核碱基，非天然存在的核碱基或核碱基结合基团，每个所述配体直接或间接地结合到所述主链中的氮原子，并且所述配位体氮原子主要彼此分离 在所述骨架中通过4至8个插入的原子; 或（b）能够与互补序列核酸杂交的核酸类似物，以形成与对应于所述类似物的所述类似物和所述核酸的常规脱氧核糖核苷酸之间的杂交体相比，通过加热变性更稳定的杂交体; 或（c）能够与双链核酸杂交的核酸类似物，其中一条链具有与所述类似物互补的序列，以便从所述一条链取代另一条链。