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公开(公告)号：US06713602B1
公开(公告)日：2004-03-30
申请号：US08462977
申请日：1995-06-05
IPC分类号： A61K3800
CPC分类号： C07H21/00 , A61K38/00 , C07K5/06026 , C07K5/06139 , C07K7/06 , C07K7/08 , C07K14/003 , C12Q1/68 , C12Q1/6813 , C12Q1/6869 , C12Q2535/107
摘要： A novel class of compounds, known as peptide nucleic acids, bind complementary ssDNA and RNA strands more strongly than a corresponding DNA. The peptide nucleic acids generally comprise ligands such as naturally occurring DNA bases attached to a peptide backbone through a suitable linker.
摘要翻译： 称为肽核酸的新一类化合物比相应的DNA更强地结合互补的ssDNA和RNA链。 肽核酸通常包含配体，例如通过合适的接头连接到肽主链上的天然存在的DNA碱基。
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2.发明授权Peptide nucleic acids having enhanced binding affinity and sequence specificity 失效具有增强的结合亲和力和序列特异性的肽核酸
公开(公告)号：US5766855A
公开(公告)日：1998-06-16
申请号：US686113
申请日：1996-07-24
申请人： Ole Buchardt, deceased , Michael Egholm , Peter Eigil Nielsen , Rolf Henrik Berg , by Dorte Buchardt, executor
发明人： Ole Buchardt, deceased , Michael Egholm , Peter Eigil Nielsen , Rolf Henrik Berg , by Dorte Buchardt, executor
CPC分类号： C07H21/00 , C07K14/003 , C07K5/06026 , C07K5/06139 , C07K7/06 , C07K7/08 , C12Q1/68 , C12Q1/6813 , C12Q1/6869 , A61K38/00 , Y10S435/81
摘要： A novel class of compounds, known as peptide nucleic acids, bind complementary DNA and RNA strands more strongly than a corresponding DNA strand, and exhibit increased sequence specificity and binding affinity. Methods of increasing binding affinity and sequence specificity of peptide nucleic acids are provided wherein some peptide nucleic acids comprise ligands selected from a group consisting of naturally-occurring nucleobases and non-naturally-occurring nucleobases attached to a polyamide backbone, while other peptide nucleic acids contain at least one 2,6-diaminopurine nucleobase and at least one C.sub.1 -C.sub.8 alkylamine side chain.
摘要翻译： 称为肽核酸的新一类化合物比相应的DNA链更强地结合互补DNA和RNA链，并表现出增加的序列特异性和结合亲和力。 提供增加肽核酸的结合亲和力和序列特异性的方法，其中一些肽核酸包含选自天然存在的核碱基和连接到聚酰胺主链的非天然存在的核碱基的配体，而其它肽核酸含有 至少一个2,6-二氨基嘌呤核碱基和至少一个C1-C8烷基胺侧链。
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3.发明授权
公开(公告)号：US5972610A
公开(公告)日：1999-10-26
申请号：US947317
申请日：1997-10-08
申请人： Ole Buchardt, deceased , by Dorte Buchardt, legal representative , Michael Egholm , Peter Eigil Nielsen , Rolf Henrik Berg , Christopher John Stanley
发明人： Ole Buchardt, deceased , by Dorte Buchardt, legal representative , Michael Egholm , Peter Eigil Nielsen , Rolf Henrik Berg , Christopher John Stanley
IPC分类号： C12N15/09 , C07H21/04 , C12Q20060101 , C12Q1/68 , G01N33/50 , G01N33/53 , G01N33/566 , C12N15/00 , C12P19/34
CPC分类号： C12Q1/6848 , C12Q1/686
摘要： The present invention relates to the use of nucleic acid analogues in blocking nucleic acid amplification procedures and to diagnostic and analytical techniques based thereon. Also included are kits for use in the conduct of nucleic acid amplification reactions.
摘要翻译： 本发明涉及核酸类似物在阻断核酸扩增程序中的用途以及基于其的诊断和分析技术。 还包括用于进行核酸扩增反应的试剂盒。
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公开(公告)号：US5837459A
公开(公告)日：1998-11-17
申请号：US653605
申请日：1996-05-24
IPC分类号： C12N15/09 , C12P19/34 , C12Q1/68 , G01N33/53 , G01N33/566
CPC分类号： C12P19/34 , C12Q1/6813 , C12Q1/6865
摘要： RNA is transcribed from a double stranded DNA template by forming a complex by hybridizing to the template at a desired transcription initiation site one or more oligonucleic acid analogues of the PNA type capable of forming a transcription initiation site with the DNA and exposing the complex to the action of a DNA dependant RNA polymerase in the presence of nucleoside triphosphates. Equal length transcripts may be obtained by placing a block to transcription downstream from the initiation site or by cutting the template at such a selected location. The initiation site is formed by displacement of one strand of the DNA locally by the PNA hybridization.
摘要翻译： 通过在期望的转录起始位点与模板杂交形成复合物，RNA可以从双链DNA模板转录成能够与DNA形成转录起始位点的一种或多种PNA型寡核酸类似物，并将该复合物暴露于 在DNA核苷三磷酸存在下DNA依赖性RNA聚合酶的作用。 可以通过将起始位点的下游位置嵌段转移到转录起始位点或通过在这样的选择位置切割模板来获得相等的长度转录物。 通过PNA杂交局部置换DNA的一条链来形成起始位点。
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公开(公告)号：US5719262A
公开(公告)日：1998-02-17
申请号：US685484
申请日：1996-07-24
申请人： Ole Buchardt, deceased , by Dorte Buchardt, legal representative , Michael Egholm , Peter Eigil Nielsen , Rolf Henrik Berg
发明人： Ole Buchardt, deceased , by Dorte Buchardt, legal representative , Michael Egholm , Peter Eigil Nielsen , Rolf Henrik Berg
CPC分类号： C07H21/00 , C07K14/003 , C07K5/06139 , C12Q1/68 , C12Q1/6869
摘要： A novel class of compounds, known as peptide nucleic acids, bind complementary DNA and RNA strands more strongly than the corresponding DNA or RNA strands, and exhibit increased sequence specificity and solubility. The peptide nucleic acids comprise ligands selected from a group consisting of naturally-occurring nucleobases and non-naturally-occurring nucleobases attached to a polyamide backbone, and contain alkylamine side chains.
摘要翻译： 称为肽核酸的新一类化合物比相应的DNA或RNA链更强地结合互补DNA和RNA链，并表现出增加的序列特异性和溶解度。 肽核酸包含选自天然存在的核碱基和连接到聚酰胺主链的非天然存在的核碱基并且含有烷基胺侧链的配体。
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6.发明申请IDENTIFICATION OF PHOSPHORYLATION SITES IN POLYPEPTIDES BY EMPLOYMENT OF URANYL PHOTOCLEAVAGE 审中-公开通过实施乌克兰电影鉴定多糖中的磷酸化位点公开(公告)号：US20100144044A1
公开(公告)日：2010-06-10
申请号：US12517164
申请日：2007-12-07
CPC分类号： G01N33/6803 , C07K14/4732 , G01N33/6842
摘要： The present invention relates to a method of cleaving a polypeptide at one or more phosphorylated residues. Said cleavage is induced by irradiation and is dependent on the presence of uranyl. The method is useful for analysis of phosphoproteoms and also for protein purification. The method also relates to a method of protein purification, wherein the phosphorylated protein is immobilized on a column said immobilization being dependent on uranyl.
摘要翻译： 本发明涉及在一个或多个磷酸化残基处切割多肽的方法。 所述切割是通过照射诱导的，并且取决于铀酰的存在。 该方法可用于磷酸化蛋白的分析和蛋白质纯化。 该方法还涉及蛋白质纯化的方法，其中将磷酸化蛋白质固定在所述固定化依赖于铀酰的柱上。
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7.发明授权
公开(公告)号：US5891625A
公开(公告)日：1999-04-06
申请号：US338535
申请日：1995-03-10
申请人： Ole Buchardt, deceased , Michael Egholm , Peter Eigil Nielsen , Rolf Henrik Berg , Christopher John Stanley
发明人： Ole Buchardt, deceased , Michael Egholm , Peter Eigil Nielsen , Rolf Henrik Berg , Christopher John Stanley
IPC分类号： C12N15/09 , C07H21/04 , C12Q20060101 , C12Q1/68 , G01N33/50 , G01N33/53 , G01N33/566 , C07H21/02 , C12P19/34
CPC分类号： C12Q1/6848 , C12Q1/686
摘要： Nucleic acid analogues such as peptide-nucleic acids (PNAs) which hybridize strongly to nucleic acids are used to inhibit nucleic acid amplification procedures such as PCR. False positives in subsequent PCR assays are prevented by hybridizing a PNA to PCR amplification products. Assays capable of discriminating between single base mutants are conducted by using a PNA hybridizing to one of the two allelic forms to inhibit a PCR amplification of that form selectively. Asymmetric PCR amplifications are carried out by starting a PCR symmetrically using like quantities of forward and reverse primers, and, once the amplification is established, disabling one primer by hybridizing a PNA thereto.
摘要翻译： PCT No.PCT / EP93 / 01435 Sec。 371 1995年3月10日 102（e）1995年3月10日PCT PCT 1993年6月7日PCT公布。 公开号WO93 / 25706 日期1993年12月23日使用与核酸强烈杂交的核酸类似物如肽 - 核酸（PNA）来抑制核酸扩增程序如PCR。 通过将PNA与PCR扩增产物杂交来防止后续PCR测定中的假阳性。 能够区分单碱基突变体的测定通过使用与两种等位基因形式中的一种杂交的PNA进行，以选择性地抑制该形式的PCR扩增。 通过使用类似量的正向引物和反向引物对称地进行PCR来进行不对称PCR扩增，一旦扩增成功，通过与PNA杂交就能使一个引物失活。
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公开(公告)号：US5786461A
公开(公告)日：1998-07-28
申请号：US847095
申请日：1997-05-01
申请人： Ole Buchardt, deceased , Michael Egholm , Peter Eigil Nielsen , Rolf Henrik Berg , Dorte Buchardt
发明人： Ole Buchardt, deceased , Michael Egholm , Peter Eigil Nielsen , Rolf Henrik Berg , Dorte Buchardt
CPC分类号： C07H21/00 , C07K14/003 , C07K5/06139 , C12Q1/68 , C12Q1/6869
摘要： A novel class of compounds, known as peptide nucleic acids, bind complementary DNA and RNA strands more strongly than the corresponding DNA or RNA strands, and exhibit increased sequence specificity and solubility. The peptide nucleic acids comprise ligands selected from a group consisting of naturally-occurring nucleobases and non-naturally-occurring nucleobases attached to a polyamide backbone, and contain alkylamine side chains.
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公开(公告)号：US07378485B2
公开(公告)日：2008-05-27
申请号：US10154890
申请日：2002-05-23
CPC分类号： C07H21/00 , A61K38/00 , C07K5/06026 , C07K5/06139 , C07K7/06 , C07K7/08 , C07K14/003 , C12Q1/68 , C12Q1/6813 , C12Q1/6832 , C12Q1/6869 , C12Q2535/107 , C12Q2525/107
摘要： A novel class of compounds, known as peptide nucleic acids, bind complementary ssDNA and RNA strands more strongly than a corresponding DNA. The peptide nucleic acids generally comprise ligands such as naturally occurring DNA bases attached to a peptide backbone through a suitable linker.
摘要翻译： 称为肽核酸的新一类化合物比相应的DNA更强地结合互补的ssDNA和RNA链。 肽核酸通常包含配体，例如通过合适的接头连接到肽主链上的天然存在的DNA碱基。
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10.发明授权Peptide nucleic acids having enhanced binding affinity, sequence specificity and solubility 失效具有增强的结合亲和力，序列特异性和溶解度的肽核酸
公开(公告)号：US5736336A
公开(公告)日：1998-04-07
申请号：US847108
申请日：1997-05-01
申请人： Ole Buchardt, deceased , by Dorte Buchardt, representative , Michael Egholm , Peter Eigil Nielsen , Rolf Henrik Berg
发明人： Ole Buchardt, deceased , by Dorte Buchardt, representative , Michael Egholm , Peter Eigil Nielsen , Rolf Henrik Berg
IPC分类号： A61K38/00 , C07H21/00 , C07K5/06 , C07K5/078 , C07K7/06 , C07K7/08 , C07K14/00 , C12Q1/68 , C07K5/00
CPC分类号： C07H21/00 , C07K14/003 , C07K5/06026 , C07K5/06139 , C07K7/06 , C07K7/08 , C12Q1/68 , C12Q1/6813 , C12Q1/6869 , A61K38/00 , Y10S435/81
摘要： A novel class of compounds, known as peptide nucleic acids, bind complementary DNA and RNA strands more strongly than a corresponding DNA strand, and exhibit increased sequence specificity and solubility. The peptide nucleic acids comprise ligands selected from a group consisting of naturally-occurring nucleobases and non-naturally-occurring nucleobases attached to a polyamide backbone, and contain C.sub.1 -C.sub.8 alkylamine side chains. Methods of enhancing the solubility, binding affinity and sequence specificity of PNAs are provided.
摘要翻译： 称为肽核酸的新一类化合物比相应的DNA链更强地结合互补DNA和RNA链，并表现出增加的序列特异性和溶解度。 肽核酸包含选自天然存在的核碱基和连接到聚酰胺主链上的非天然存在的核碱基并含有C 1 -C 8烷基胺侧链的配体。 提供了增强PNA的溶解度，结合亲和力和序列特异性的方法。
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