公开(公告)号：US06956102B2

公开(公告)日：2005-10-18

申请号：US09945901

申请日：2001-09-04

申请人： Lorrie P. Daggett ,  Steven B. Ellis ,  Chen Wang Liaw ,  Chin-Chun Lu

发明人： Lorrie P. Daggett ,  Steven B. Ellis ,  Chen Wang Liaw ,  Chin-Chun Lu

IPC分类号： C12N15/09 ,  A61K38/00 ,  C07K14/705 ,  C07K16/28 ,  C12N5/10 ,  C12N15/12 ,  C12P21/02 ,  C12P21/08 ,  C12Q1/02 ,  C12Q1/68 ,  C12R1/91 ,  G01N33/50 ,  G01N33/68

CPC分类号： G01N33/5008 ,  A01K2217/05 ,  A61K38/00 ,  C07K14/70571 ,  G01N33/502 ,  G01N33/5041 ,  G01N33/5058 ,  G01N33/5091 ,  G01N33/6872 ,  G01N2333/70571

摘要： Provided herein are nucleic acids encoding human NMDA receptor protein subunits and the proteins encoded thereby. In one aspect of the invention, the nucleic acids encode provides In a preferred embodiment, the invention nucleic acids encode MNDAR1, NMDAR2A, NMDAR2B, NMDAR2C and NMDAR2D subunits of human NMDA receptors. The disclosed nucleic acids are also useful as probes, thus enabling those skilled in the art, to identify and isolate related human receptor subunits. Functional glutamate receptors can be assembled, in accordance with the present invention, from a plurality of one type of NMDA receptor subunit protein (homomeric) or from a mixture of two or more types of subunit proteins (heteromeric). Also provided are methods for using the disclosed receptor subunits to identify and characterize compounds which affect the function of such receptors, e.g., agonists, antagonists, and modulators of glutamate receptor function. Methods for determining whether unknown protein(s) are functional as NMDA receptor subunits are also provided.

摘要翻译： 本文提供了编码人NMDA受体蛋白质亚基的核酸和由此编码的蛋白质。 在本发明的一个方面，核酸编码提供。在优选的实施方案中，本发明的核酸编码人NMDA受体的MNDAR1，NMDAR2A，NMDAR2B，NMDAR2C和NMDAR2D亚基。 所公开的核酸也可用作探针，因此使得本领域技术人员能够鉴定和分离相关的人受体亚单位。 功能性谷氨酸受体可以根据本发明从多种一种NMDA受体亚基蛋白（同源性）或两种或更多种亚基蛋白（异聚体）的混合物组装。 还提供了使用所公开的受体亚基来鉴定和表征影响这些受体功能的化合物的方法，例如激动剂，拮抗剂和谷氨酸受体功能的调节剂。 还提供了用于确定未知蛋白质是否作为NMDA受体亚单位起作用的方法。

公开(公告)号：US6033865A

公开(公告)日：2000-03-07

申请号：US480474

申请日：1995-06-06

申请人： Lorrie P. Daggett ,  Steven B. Ellis ,  Chen Wang Liaw

发明人： Lorrie P. Daggett ,  Steven B. Ellis ,  Chen Wang Liaw

IPC分类号： C12N15/09 ,  A61K38/00 ,  C07K14/705 ,  C07K16/28 ,  C12N5/10 ,  C12N15/12 ,  C12P21/02 ,  C12P21/08 ,  C12Q1/02 ,  C12Q1/68 ,  C12R1/91 ,  G01N33/50 ,  G01N33/68 ,  C12N15/10 ,  C07K1/00 ,  G01N33/566

CPC分类号： G01N33/5008 ,  C07K14/70571 ,  G01N33/502 ,  G01N33/5041 ,  G01N33/5058 ,  G01N33/5091 ,  G01N33/6872 ,  A01K2217/05 ,  A61K38/00 ,  G01N2333/70571

摘要： In accordance with the present invention, there are provided nucleic acids encoding human NMDA receptor protein subunits and the proteins encoded thereby. The NMDA receptor subunits of the invention comprise components of NMDA receptors that have cation-selective channels and bind glutamate and NMDA. In one aspect of the invention, the nucleic acids encode NMDAR1 and NMDAR2 subunits of human NMDA receptors. In a preferred embodiment, the invention nucleic acids encode NMDAR1, NMDAR2A and NMDAR2C subunits of human NMDA receptors. In addition to being useful for the production of NMDA receptor subunit proteins, these nucleic acids are also useful as probes, thus enabling those skilled in the art, without undue experimentation, to identify and isolate related human receptor subunits. Functional glutamate receptors can be assembled, in accordance with the present invention, from a plurality of one type of NMDA receptor subunit protein (homomeric) or from a mixture of two or more types of subunit proteins (heteromeric). In addition to disclosing novel NMDA receptor protein subunits, the present invention also comprises methods for using such receptor subunits to identify and characterize compounds which affect the function of such receptors, e.g., agonists, antagonists, and modulators of glutamate receptor function. The invention also comprises methods for determining whether unknown protein(s) are functional as NMDA receptor subunits.

摘要翻译： 根据本发明，提供了编码人NMDA受体蛋白质亚基的核酸和由此编码的蛋白质。 本发明的NMDA受体亚基包含具有阳离子选择性通道并结合谷氨酸和NMDA的NMDA受体的组分。 在本发明的一个方面，核酸编码人NMDA受体的NMDAR1和NMDAR2亚基。 在优选的实施方案中，本发明核酸编码人NMDA受体的NMDAR1，NMDAR2A和NMDAR2C亚基。 除了可用于产生NMDA受体亚基蛋白之外，这些核酸也可用作探针，因此使得本领域技术人员无需过多的实验来鉴定和分离相关的人受体亚单位。 功能性谷氨酸受体可以根据本发明从多种一种NMDA受体亚基蛋白（同源性）或两种或更多种亚基蛋白（异聚体）的混合物组装。 除了公开新的NMDA受体蛋白质亚基之外，本发明还包括使用这些受体亚基来鉴定和表征影响这些受体功能的化合物的方法，例如激动剂，拮抗剂和谷氨酸受体功能的调节剂。 本发明还包括用于确定未知蛋白质是否用作NMDA受体亚基的方法。

公开(公告)号：US5807689A

公开(公告)日：1998-09-15

申请号：US486270

申请日：1995-06-06

申请人： Lorrie Daggett ,  Steven B. Ellis ,  Chen Liaw ,  Aaron Pontsler ,  Edwin C. Johnson ,  Stephen D. Hess

发明人： Lorrie Daggett ,  Steven B. Ellis ,  Chen Liaw ,  Aaron Pontsler ,  Edwin C. Johnson ,  Stephen D. Hess

IPC分类号： G01N33/566 ,  A61K39/395 ,  A61K45/00 ,  A61P25/00 ,  A61P25/08 ,  A61P25/28 ,  A61P43/00 ,  C07K14/705 ,  C07K16/28 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/00 ,  C12N5/10 ,  C12N15/02 ,  C12N15/09 ,  C12N15/12 ,  C12P21/02 ,  C12P21/08 ,  C12Q1/02 ,  C12Q1/68 ,  C12R1/91 ,  G01N33/15 ,  G01N33/50 ,  G01N33/53 ,  G01N33/68 ,  C12N15/63

CPC分类号： G01N33/5091 ,  C07K14/70571 ,  G01N33/5008 ,  G01N33/502 ,  G01N33/5058 ,  G01N33/6872 ,  A01K2217/05 ,  G01N2333/70571

摘要： In accordance with the present invention, there are provided nucleic acids encoding human metabotropic glutamate receptor subtypes and the proteins encoded thereby. In a particular embodiment, the invention nucleic acids encode mGluR1, mGluR2, mGluR3 and mGluR5 subtypes of human metabotropic glutamate receptors. In addition to being useful for the production of metabotropic glutamate receptor subtypes, these nucleic acids are also useful as probes, thus enabling those skilled in the art, without undue experimentation, to identify and isolate related human receptor subunits. In addition to disclosing novel metabotropic glutamate receptor subtypes, the present invention also comprises methods for using such receptor subtypes to identify and characterize compounds which affect the function of such receptors, e.g., agonists, antagonists, and modulators of glutamate receptor function.

摘要翻译： 根据本发明，提供了编码人代谢型谷氨酸受体亚型的核酸和由此编码的蛋白质。 在一个具体实施方案中，本发明的核酸编码人代谢型谷氨酸受体的mGluR1，mGluR2，mGluR3和mGluR5亚型。 除了可用于代谢型谷氨酸受体亚型的生产之外，这些核酸还可用作探针，因此使得本领域技术人员无需过度的实验来鉴定和分离相关的人受体亚单位。 除了揭示新型代谢型谷氨酸受体亚型之外，本发明还包括使用这种受体亚型鉴定和表征影响这些受体功能的化合物的方法，例如激动剂，拮抗剂和谷氨酸受体功能的调节剂。

公开(公告)号：US06521413B1

公开(公告)日：2003-02-18

申请号：US09386123

申请日：1999-08-30

申请人： Lorrie P. Daggett ,  Steven B. Ellis ,  Chen Wang Liaw ,  Chin-Chun Lu

发明人： Lorrie P. Daggett ,  Steven B. Ellis ,  Chen Wang Liaw ,  Chin-Chun Lu

IPC分类号： G01N3353

CPC分类号： G01N33/5008 ,  A01K2217/05 ,  A61K38/00 ,  C07K14/70571 ,  G01N33/502 ,  G01N33/5041 ,  G01N33/5058 ,  G01N33/5091 ,  G01N33/6872 ,  G01N2333/70571

摘要： In accordance with the present invention, there are provided nucleic acids encoding human NMDA receptor protein subunits and the proteins encoded thereby. The NMDA receptor subunits of the invention comprise components of NMDA receptors that have cation-selective channels and bind glutamate and NMDA. In one aspect of the invention, the nucleic acids encode NMDAR1 and NMDAR2 subunits of human NMDA receptors. In a preferred embodiment, the invention nucleic acids encode NMDAR1, NMDAR2A, NMDAR2B, NMDAR2C and NMDAR2D subunits of human NMDA receptors. In addition to being useful for the production of NMDA receptor subunit proteins, these nucleic acids are also useful as probes, thus enabling those skilled in the art, without undue experimentation, to identify and isolate related human receptor subunits. Functional glutamate receptors can be assembled, in accordance with the present invention, from a plurality of one type of NMDA receptor subunit protein (homomeric) or from a mixture of two or more types of subunit proteins (heteromeric). In addition to disclosing novel NMDA receptor protein subunits, the present invention also comprises methods for using such receptor subunits to identify and characterize compounds which affect the function of such receptors, e.g., agonists, antagonists, and modulators of glutamate receptor function. The invention also comprises methods for determining whether unknown protein(s) are functional as NMDA receptor subunits.

摘要翻译： 根据本发明，提供了编码人NMDA受体蛋白质亚基的核酸和由此编码的蛋白质。 本发明的NMDA受体亚基包含具有阳离子选择性通道并结合谷氨酸和NMDA的NMDA受体的组分。 在本发明的一个方面，核酸编码人NMDA受体的NMDAR1和NMDAR2亚基。 在优选的实施方案中，本发明核酸编码人NMDA受体的NMDAR1，NMDAR2A，NMDAR2B，NMDAR2C和NMDAR2D亚基。 除了可用于产生NMDA受体亚基蛋白之外，这些核酸也可用作探针，因此使得本领域技术人员无需过多的实验来鉴定和分离相关的人受体亚单位。 功能性谷氨酸受体可以根据本发明从多种一种NMDA受体亚基蛋白（同源性）或两种或更多种亚基蛋白（异聚体）的混合物组装。 除了公开新的NMDA受体蛋白质亚基之外，本发明还包括使用这些受体亚基来鉴定和表征影响这些受体功能的化合物的方法，例如激动剂，拮抗剂和谷氨酸受体功能的调节剂。 本发明还包括用于确定未知蛋白质是否用作NMDA受体亚基的方法。

公开(公告)号：US06485919B1

公开(公告)日：2002-11-26

申请号：US09459715

申请日：1999-12-13

申请人： Lorrie Daggett ,  Steven B. Ellis ,  Chen Liaw ,  Aaron Pontsler ,  Edwin C. Johnson ,  Stephen D. Hess

发明人： Lorrie Daggett ,  Steven B. Ellis ,  Chen Liaw ,  Aaron Pontsler ,  Edwin C. Johnson ,  Stephen D. Hess

IPC分类号： G01N33567

CPC分类号： G01N33/5091 ,  A01K2217/05 ,  C07K14/70571 ,  G01N33/5008 ,  G01N33/502 ,  G01N33/5058 ,  G01N33/6872 ,  G01N2333/70571

摘要： In accordance with the present invention, there are provided nucleic acids encoding human metabotropic glutamate receptor subtypes and the proteins encoded thereby. In a particular embodiment, the invention nucleic acids encode mGluR1, mGluR2, mGluR3 and mGluR5 subtypes of human metabotropic glutamate receptors. In addition to being useful for the production of metabotropic glutamate receptor subtypes, these nucleic acids are also useful as probes, thus enabling those skilled in the art, without undue experimentation, to identify and isolate related human receptor subunits. In addition to disclosing novel metabotropic glutamate receptor subtypes, the present invention also comprises methods for using such receptor subtypes to identify and characterize compounds which affect the function of such receptors, e.g., agonists, antagonists, and modulators of glutamate receptor function.

摘要翻译： 根据本发明，提供了编码人代谢型谷氨酸受体亚型的核酸和由此编码的蛋白质。 在一个具体实施方案中，本发明的核酸编码人代谢型谷氨酸受体的mGluR1，mGluR2，mGluR3和mGluR5亚型。 除了可用于代谢型谷氨酸受体亚型的生产之外，这些核酸还可用作探针，因此使得本领域技术人员无需过度的实验来鉴定和分离相关的人受体亚单位。 除了揭示新型代谢型谷氨酸受体亚型之外，本发明还包括使用这种受体亚型鉴定和表征影响这些受体功能的化合物的方法，例如激动剂，拮抗剂和谷氨酸受体功能的调节剂。