公开(公告)号：US20200002719A1

公开(公告)日：2020-01-02

申请号：US16573538

申请日：2019-09-17

Applicant: Monsanto Technology LLC

Inventor： James A. Baum ,  Thomas Cerruti ,  Stanislaw Flasinski ,  Xiaoran Fu ,  Arlene R. Howe ,  Sara Ann Salvador

IPC: C12N15/82 ,  C07K14/325 ,  A01N47/08 ,  A01N63/02

Abstract: Engineered Cry1Da amino acid sequences are provided that exhibit improved Lepidopteran insecticidal activity and an enhanced Lepidopteran spectrum compared to the naturally occurring Cry1Da protein toxin. Polynucleotide sequences intended for use in expression of the improved proteins in plants are also provided. Particular embodiments provide compositions containing insect inhibitory amounts of the engineered proteins, as well as recombinant plants, plant parts, and seeds containing polynucleotide constructs encoding one or more of the improved engineered proteins.

公开(公告)号：US20180334684A1

公开(公告)日：2018-11-22

申请号：US16047187

申请日：2018-07-27

Applicant: Monsanto Technology LLC

Inventor： James A. Baum ,  Thomas Cerruti ,  Stanislaw Flasinski ,  Xiaoran Fu ,  Arlene R. Howe ,  Sara Ann Salvador

IPC: C12N15/82 ,  A01N63/02 ,  C07K14/325 ,  A01N47/08

Abstract: Engineered Cry1Da amino acid sequences are provided that exhibit improved Lepidopteran insecticidal activity and an enhanced Lepidopteran spectrum compared to the naturally occurring Cry1Da protein toxin. Polynucleotide sequences intended for use in expression of the improved proteins in plants are also provided. Particular embodiments provide compositions containing insect inhibitory amounts of the engineered proteins, as well as recombinant plants, plant parts, and seeds containing polynucleotide constructs encoding one or more of the improved engineered proteins.

公开(公告)号：US10059959B2

公开(公告)日：2018-08-28

申请号：US14884432

申请日：2015-10-15

Applicant: Monsanto Technology LLC

Inventor： James A. Baum ,  Thomas Cerruti ,  Stanislaw Flasinski ,  Xiaoran Fu ,  Arlene R. Howe ,  Sara Ann Salvador

IPC: C07K14/325 ,  C12N15/82 ,  A01H5/10 ,  C12N15/32 ,  A01N47/08 ,  A01N63/02

Abstract: Engineered Cry1Da amino acid sequences are provided that exhibit improved Lepidopteran insecticidal activity and an enhanced Lepidopteran spectrum compared to the naturally occurring Cry1Da protein toxin. Polynucleotide sequences intended for use in expression of the improved proteins in plants are also provided. Particular embodiments provide compositions containing insect inhibitory amounts of the engineered proteins, as well as recombinant plants, plant parts, and seeds containing polynucleotide constructs encoding one or more of the improved engineered proteins.

公开(公告)号：US20180127774A1

公开(公告)日：2018-05-10

申请号：US15849012

申请日：2017-12-20

Applicant: Monsanto Technology LLC

Inventor： James A. Baum ,  Thomas A. Cerruti ,  Crystal L. Dart ,  Leigh H. English ,  Stanislaw Flasinski ,  Xiaoran Fu ,  Victor M. Guzov ,  Arlene R. Howe ,  Jay P. Morgenstern ,  James K. Roberts ,  Sara A. Salvador ,  Jinling Wang

IPC: C12N15/82

Abstract: Nucleotide sequences are disclosed that encode novel chimeric insecticidal proteins exhibiting Lepidopteran inhibitory activity. Particular embodiments provide compositions and transformed plants, plant parts, and seeds containing the recombinant nucleic acid molecules encoding one or more of the chimeric insecticidal proteins.

公开(公告)号：US20160108426A1

公开(公告)日：2016-04-21

申请号：US14884432

申请日：2015-10-15

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： James A. Baum ,  Thomas Cerruti ,  Stanislaw Flasinski ,  Xiaoran Fu ,  Arlene R. Howe ,  Sara Ann Salvador

IPC: C12N15/82 ,  A01N63/02 ,  A01N47/08 ,  C07K14/325

CPC classification number: C12N15/8286 ,  A01N47/08 ,  A01N63/02 ,  C07K14/325 ,  Y02A40/162

Abstract: Engineered Cry1Da amino acid sequences are provided that exhibit improved Lepidopteran insecticidal activity and an enhanced Lepidopteran spectrum compared to the naturally occurring Cry1Da protein toxin. Polynucleotide sequences intended for use in expression of the improved proteins in plants are also provided. Particular embodiments provide compositions containing insect inhibitory amounts of the engineered proteins, as well as recombinant plants, plant parts, and seeds containing polynucleotide constructs encoding one or more of the improved engineered proteins.

Abstract translation: 提供了与天然存在的Cry1Da蛋白毒素相比表现出改进的鳞翅目杀虫活性和增强的鳞翅目光谱的工程化Cry1Da氨基酸序列。 还提供了用于在植物中表达改良蛋白质的多核苷酸序列。 具体实施方案提供了含有昆虫抑制量的工程改造的蛋白质的组合物，以及含有编码一种或多种改进的工程化蛋白质的多核苷酸构建体的重组植物，植物部分和种子。

公开(公告)号：US20160108422A1

公开(公告)日：2016-04-21

申请号：US14871768

申请日：2015-09-30

Applicant: Monsanto Technology LLC

Inventor： Christine M. Ellis ,  Artem G. Evdokimov ,  Paul C.C. Feng ,  Xiaoran Fu ,  Clayton T. Larue ,  Jeffrey R. Nageotte ,  Andrew C. Read ,  Lei Shi ,  Andrew M. Wollacott

IPC: C12N15/82 ,  A01N57/00 ,  C12N9/02

CPC classification number: C12N15/8274 ,  A01H5/00 ,  A01H5/10 ,  A01N57/00 ,  A01N63/02 ,  C07K14/195 ,  C07K2319/08 ,  C12N9/0071 ,  C12Y113/00 ,  C12Y114/11033

Abstract: Polypeptides and recombinant DNA molecules useful for conferring tolerance to AOPP herbicides, phenoxy acid herbicides, and pyridinyloxy acid herbicides are provided in the present invention, as well as herbicide tolerant transgenic plants, seeds, cells, and plant parts containing the recombinant DNA molecules, as well as methods of using the same.

Abstract translation: 在本发明中提供了可用于赋予对AOPP除草剂，苯氧基酸除草剂和吡啶氧基酸除草剂的耐受性的多肽和重组DNA分子，以及含有除草剂耐受性转基因植物，种子，细胞和含有重组DNA分子的植物部分，作为 以及使用相同的方法。