公开(公告)号：US07569228B2

公开(公告)日：2009-08-04

申请号：US11488803

申请日：2006-07-18

申请人： Paul Howley ,  Sonja Leyer ,  Eva Felder

发明人： Paul Howley ,  Sonja Leyer ,  Eva Felder

IPC分类号： A61K39/12 ,  A61K39/21 ,  A61K39/275

CPC分类号： C12N15/86 ,  A61K39/12 ,  A61K39/21 ,  A61K2039/5256 ,  A61K2039/53 ,  C07K14/005 ,  C07K2319/00 ,  C07K2319/40 ,  C12N2710/24143 ,  C12N2740/16322 ,  C12N2740/16334

摘要： Nucleic acids are disclosed encoding fusion proteins comprising the amino acid sequence of at least four non-attenuated HIV proteins selected from the group consisting of Vif, Vpr, Vpu, Vpx, Rev, Tat, and Nef, wherein the fusion protein does not contain a specific cleavage sequence for a cellular protease. Also disclosed are vectors comprising the nucleic acids and methods of preparing the fusion protein by transfecting a host cell with the nucleic acids or with the vectors containing the nucleic acids, expressing the fusion proteins, and recovering the fusion proteins.

摘要翻译： 公开了编码融合蛋白的核酸，其包含选自Vif，Vpr，Vpu，Vpx，Rev，Tat和Nef的至少四种非减毒HIV蛋白的氨基酸序列，其中所述融合蛋白不含有 细胞蛋白酶的特异性切割序列。 还公开了包含核酸的载体和通过用核酸转染宿主细胞或与含有核酸的载体，表达融合蛋白并回收融合蛋白来制备融合蛋白的方法。

公开(公告)号：US20060257974A1

公开(公告)日：2006-11-16

申请号：US11488803

申请日：2006-07-18

申请人： Paul Howley ,  Sonja Leyrer ,  Eva Felder

发明人： Paul Howley ,  Sonja Leyrer ,  Eva Felder

IPC分类号： C07K14/16 ,  C07H21/04 ,  C12P21/04

CPC分类号： C12N15/86 ,  A61K39/12 ,  A61K39/21 ,  A61K2039/5256 ,  A61K2039/53 ,  C07K14/005 ,  C07K2319/00 ,  C07K2319/40 ,  C12N2710/24143 ,  C12N2740/16322 ,  C12N2740/16334

摘要： Nucleic acids are disclosed encoding fusion proteins comprising the amino acid sequence of at least four non-attenuated HIV proteins selected from the group consisting of Vif, Vpr, Vpu, Vpx, Rev, Tat, and Nef, wherein the fusion protein does not contain a specific cleavage sequence for a cellular protease. Also disclosed are vectors comprising the nucleic acids and methods of preparing the fusion protein by transfecting a host cell with the nucleic acids or with the vectors containing the nucleic acids, expressing the fusion proteins, and recovering the fusion proteins.

摘要翻译： 公开了编码融合蛋白的核酸，其包含选自Vif，Vpr，Vpu，Vpx，Rev，Tat和Nef的至少四种非减毒HIV蛋白的氨基酸序列，其中所述融合蛋白不含有 细胞蛋白酶的特异性切割序列。 还公开了包含核酸的载体和通过用核酸转染宿主细胞或与含有核酸的载体，表达融合蛋白并回收融合蛋白来制备融合蛋白的方法。

公开(公告)号：US20050222388A1

公开(公告)日：2005-10-06

申请号：US10514740

申请日：2003-05-14

申请人： Paul Howley ,  Sonja Leyrer ,  Eva Felder

发明人： Paul Howley ,  Sonja Leyrer ,  Eva Felder

IPC分类号： A61K39/00 ,  A61K39/21 ,  C07K14/16 ,  C12N15/863

CPC分类号： C12N15/86 ,  A61K39/12 ,  A61K39/21 ,  A61K2039/5256 ,  A61K2039/53 ,  C07K14/005 ,  C07K2319/00 ,  C07K2319/40 ,  C12N2710/24143 ,  C12N2740/16322 ,  C12N2740/16334

摘要： The invention relates to fusion proteins comprising the amino acid sequence of at least four HIV proteins selected from Vif, Vpr, Vpu, Rev, Tat and Nef or derivatives of the amino acid sequence of one or more of said proteins, wherein the fusion protein is not processed to individual HIV proteins having the natural N and C termini. The invention further concerns nucleic acids encoding said proteins, vectors comprising said nucleic acids, and methods for producing said proteins. The fusion protein, nucleic acids and vectors are usable as vaccines for the at least partial prophylaxis against HIV infections.

摘要翻译： 本发明涉及包含选自Vif，Vpr，Vpu，Rev，Tat和Nef的至少四种HIV蛋白质的氨基酸序列或一种或多种所述蛋白质的氨基酸序列的衍生物的融合蛋白，其中融合蛋白是 没有加工成具有天然N和C末端的单个HIV蛋白质。 本发明还涉及编码所述蛋白质的核酸，包含所述核酸的载体和用于产生所述蛋白质的方法。 融合蛋白，核酸和载体可用作用于至少部分预防HIV感染的疫苗。

公开(公告)号：US08435535B2

公开(公告)日：2013-05-07

申请号：US13372723

申请日：2012-02-14

申请人： Paul Howley ,  Sonja Leyrer ,  Eva Felder

发明人： Paul Howley ,  Sonja Leyrer ,  Eva Felder

IPC分类号： A61K39/00 ,  A61K39/12 ,  A61K39/21 ,  C12P1/00 ,  C12P21/04 ,  C12N5/00

CPC分类号： C12N15/86 ,  A61K39/12 ,  A61K39/21 ,  A61K2039/5256 ,  A61K2039/53 ,  C07K14/005 ,  C07K2319/00 ,  C07K2319/40 ,  C12N2710/24143 ,  C12N2740/16322 ,  C12N2740/16334

摘要： The invention relates to fusion proteins comprising the amino acid sequence of at least three HIV proteins selected from Vif, Vpr, Vpu, Rev, and Tat or derivatives of the amino acid sequence of one or more of said proteins, wherein the fusion protein is not processed to individual HIV proteins having the natural N and C termini. The invention further concerns nucleic acids encoding said proteins, vectors comprising said nucleic acids, and methods for producing said proteins. The fusion protein, nucleic acids and vectors are usable as vaccines for the at least partial prophylaxis against HIV infections.

摘要翻译： 本发明涉及包含选自Vif，Vpr，Vpu，Rev和Tat的至少三种HIV蛋白质的氨基酸序列或一种或多种所述蛋白质的氨基酸序列的衍生物的融合蛋白，其中融合蛋白不是 加工成具有天然N和C末端的单独的HIV蛋白质。 本发明还涉及编码所述蛋白质的核酸，包含所述核酸的载体和用于产生所述蛋白质的方法。 融合蛋白，核酸和载体可用作用于至少部分预防HIV感染的疫苗。

公开(公告)号：US08147839B2

公开(公告)日：2012-04-03

申请号：US12611164

申请日：2009-11-03

申请人： Paul Howley ,  Sonja Leyrer ,  Eva Felder

发明人： Paul Howley ,  Sonja Leyrer ,  Eva Felder

IPC分类号： A61K39/12 ,  A61K39/21 ,  A61K39/00 ,  A61K39/275 ,  C12N15/00

CPC分类号： C12N15/86 ,  A61K39/12 ,  A61K39/21 ,  A61K2039/5256 ,  A61K2039/53 ,  C07K14/005 ,  C07K2319/00 ,  C07K2319/40 ,  C12N2710/24143 ,  C12N2740/16322 ,  C12N2740/16334

摘要： The invention relates to fusion proteins comprising the amino acid sequence of at least three HIV proteins selected from Vif, Vpr, Vpu, Rev, and Tat or derivatives of the amino acid sequence of one or more of said proteins, wherein the fusion protein is not processed to individual HIV proteins having the natural N and C termini. The invention further concerns nucleic acids encoding said proteins, vectors comprising said nucleic acids, and methods for producing said proteins. The fusion protein, nucleic acids and vectors are usable as vaccines for the at least partial prophylaxis against HIV infections.

摘要翻译： 本发明涉及包含选自Vif，Vpr，Vpu，Rev和Tat的至少三种HIV蛋白质的氨基酸序列或一种或多种所述蛋白质的氨基酸序列的衍生物的融合蛋白，其中融合蛋白不是 加工成具有天然N和C末端的单独的HIV蛋白质。 本发明还涉及编码所述蛋白质的核酸，包含所述核酸的载体和用于产生所述蛋白质的方法。 融合蛋白，核酸和载体可用作用于至少部分预防HIV感染的疫苗。

公开(公告)号：US08137931B2

公开(公告)日：2012-03-20

申请号：US12611188

申请日：2009-11-03

申请人： Paul Howley ,  Sonja Leyrer ,  Eva Felder

发明人： Paul Howley ,  Sonja Leyrer ,  Eva Felder

IPC分类号： A61K39/12 ,  A61K39/21 ,  A61K39/00 ,  A61K39/275 ,  C12N15/00

CPC分类号： C12N15/86 ,  A61K39/12 ,  A61K39/21 ,  A61K2039/5256 ,  A61K2039/53 ,  C07K14/005 ,  C07K2319/00 ,  C07K2319/40 ,  C12N2710/24143 ,  C12N2740/16322 ,  C12N2740/16334

摘要： The invention relates to fusion proteins comprising the amino acid sequence of at least three HIV proteins selected from Vif, Vpr, Vpu, Rev, and Tat or derivatives of the amino acid sequence of one or more of said proteins, wherein the fusion protein is not processed to individual HIV proteins having the natural N and C termini. The invention further concerns nucleic acids encoding said proteins, vectors comprising said nucleic acids, and methods for producing said proteins. The fusion protein, nucleic acids and vectors are usable as vaccines for the at least partial prophylaxis against HIV infections.

公开(公告)号：US20100047268A1

公开(公告)日：2010-02-25

申请号：US12611164

申请日：2009-11-03

申请人： Paul HOWLEY ,  Sonja Leyrer ,  Eva Felder

发明人： Paul HOWLEY ,  Sonja Leyrer ,  Eva Felder

IPC分类号： A61K39/21 ,  A61P37/04 ,  A61P31/18

CPC分类号： C12N15/86 ,  A61K39/12 ,  A61K39/21 ,  A61K2039/5256 ,  A61K2039/53 ,  C07K14/005 ,  C07K2319/00 ,  C07K2319/40 ,  C12N2710/24143 ,  C12N2740/16322 ,  C12N2740/16334

摘要： The invention relates to fusion proteins comprising the amino acid sequence of at least three HIV proteins selected from Vif, Vpr, Vpu, Rev, and Tat or derivatives of the amino acid sequence of one or more of said proteins, wherein the fusion protein is not processed to individual HIV proteins having the natural N and C termini. The invention further concerns nucleic acids encoding said proteins, vectors comprising said nucleic acids, and methods for producing said proteins. The fusion protein, nucleic acids and vectors are usable as vaccines for the at least partial prophylaxis against HIV infections.

公开(公告)号：US20090280561A1

公开(公告)日：2009-11-12

申请号：US12490424

申请日：2009-06-24

申请人： Paul Howley ,  Sonja Leyrer ,  Eva Felder

发明人： Paul Howley ,  Sonja Leyrer ,  Eva Felder

IPC分类号： C12N5/00 ,  C12N15/63

CPC分类号： C12N15/86 ,  A61K39/12 ,  A61K39/21 ,  A61K2039/5256 ,  A61K2039/53 ,  C07K14/005 ,  C07K2319/00 ,  C07K2319/40 ,  C12N2710/24143 ,  C12N2740/16322 ,  C12N2740/16334

摘要： The invention relates to fusion proteins comprising the amino acid sequence of at least three HIV proteins selected from Vif, Vpr, Vpu, Rev, and Tat or derivatives of the amino acid sequence of one or more of said proteins, wherein the fusion protein is not processed to individual HIV proteins having the natural N and C termini. The invention further concerns nucleic acids encoding said proteins, vectors comprising said nucleic acids, and methods for producing said proteins. The fusion protein, nucleic acids and vectors are usable as vaccines for the at least partial prophylaxis against HIV infections.

摘要翻译： 本发明涉及包含选自Vif，Vpr，Vpu，Rev和Tat的至少三种HIV蛋白质的氨基酸序列或一种或多种所述蛋白质的氨基酸序列的衍生物的融合蛋白，其中融合蛋白不是 加工成具有天然N和C末端的单独的HIV蛋白质。 本发明还涉及编码所述蛋白质的核酸，包含所述核酸的载体和用于产生所述蛋白质的方法。 融合蛋白，核酸和载体可用作用于至少部分预防HIV感染的疫苗。