公开(公告)号：US5460952A

公开(公告)日：1995-10-24

申请号：US973324

申请日：1992-11-04

Applicant: Su-May Yu ,  Li-Fei Liu

Inventor： Su-May Yu ,  Li-Fei Liu

IPC: C12N15/09 ,  A01H5/00 ,  C12N5/00 ,  C12N5/10 ,  C12N15/82 ,  C12P21/02 ,  C12R1/91 ,  C12P21/06 ,  C12N15/00

CPC classification number: C12N15/8238 ,  C12N15/8222 ,  C12N15/8234

Abstract: Disclosed is a method for producing a gene product by expressing a gene encoding said gene product in plant host cells, comprising the steps of: constructing a vector expressible in plant host cells, the vector comprising a promoter region derived from an .alpha.-amylase gene of a plant, and a gene encoding a desired gene product; transforming a compatible plant host cell with the vector; cultivating the resultant transformant host cell; subjecting the cultivated transformant host cell to a sugar-depleted or sugar-free condition to promote the expression of the gene under the control of the promoter region; and recovering the expressed gene product. Also disclosed is a method for producing a gene product method which comprises the steps of: constructing a vector expressible in plant host cells, the vector comprising a promoter region derived from an .alpha.-amylase gene of a plant, and a gene encoding a desired gene product, the promoter region including the promoter and a DNA sequence encoding the signal peptide; transforming a compatible plant host cell with the vector; cultivating the resultant transformant host cell in a suitable culture medium; and directly recovering the expressed gene product from the medium.

Abstract translation: 公开了通过在植物宿主细胞中表达编码所述基因产物的基因来产生基因产物的方法，包括以下步骤：构建在植物宿主细胞中可表达的载体，所述载体包含衍生自α-淀粉酶基因的启动子区 植物和编码所需基因产物的基因; 用载体转化相容的植物宿主细胞; 培养所得转化体宿主细胞; 对培养的转化体宿主细胞进行糖消化或无糖条件以促进基因在启动子区域控制下的表达; 并回收表达的基因产物。 还公开了一种生产基因产物方法的方法，其包括以下步骤：构建在植物宿主细胞中可表达的载体，所述载体包含源自植物的α-淀粉酶基因的启动子区域和编码所需基因的基因 产物，包括启动子的启动子区域和编码信号肽的DNA序列; 用载体转化相容的植物宿主细胞; 在合适的培养基中培养所得的转化体宿主细胞; 并从培养基中直接回收表达的基因产物。