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    Gibberellin 2-oxidase genes and uses thereof
    4.
    发明授权
    Gibberellin 2-oxidase genes and uses thereof 有权
    赤霉素2-氧化酶基因及其用途

    公开(公告)号：US08034992B2

    公开(公告)日：2011-10-11

    申请号：US12139674

    申请日：2008-06-16

    Applicant: Su-May Yu ,  Shuen-Fang Lo ,  Liang-Jwu Chen

    Inventor： Su-May Yu ,  Shuen-Fang Lo ,  Liang-Jwu Chen

    IPC: A01H5/00 ,  A01H1/00 ,  C07H21/04 ,  C12N5/04 ,  C12N15/05

    CPC classification number: C12N15/8297 ,  C12N9/0071

    Abstract: Novel gibberellin 2-oxidase (GA2ox) genes were identified. Differential expression of GA2ox genes correlated with flower development, seed germination, tiller growth and other developmental processes. In addition, the early and increased growth of tiller and adventitious root and altered root architecture caused by overexpression of GA2oxs further suggest the pleiotropic role of GA2oxs in controlling growth and architecture in plants such as rice. GA2ox5, GA2ox6 and GA2ox9 were three genes encoding class C20 GA2oxs in rice. Mutants or transgenic rice overexpressing class C20 GA2oxs exhibited a broad range of mutant phenotypes, including semi-dwarfism, increased root system and higher tiller numbers that may favor grain yield. Mutations in the conserved domain III were found to affect the physiological activity of class C20 GA2oxs.

    Abstract translation: 鉴定了新型赤霉素2-氧化酶（GA2ox）基因。 GA2ox基因的差异表达与花的发育，种子发芽，分蘖生长等发育过程相关。 此外，由于GA2ox过表达引起的分蘖和不定根的早期增长和根系的改变进一步表明GA2oxs在控制水稻等植物生长和结构方面具有多方位的作用。 GA2ox5，GA2ox6和GA2ox9是水稻中编码C20 GA2oxs的三个基因。 突变体或转基因水稻超过C20类型的GA2oxs表现出广泛的突变表型，包括半侏儒症，增加的根系和较高的分蘖数，可能有利于谷物产量。 发现保守结构域III中的突变会影响C20类GA2氧化酶的生理活性。

    ABIOTIC STRESS TOLERANT TRANSGENIC PLANTS
    6.
    发明申请
    ABIOTIC STRESS TOLERANT TRANSGENIC PLANTS 有权
    生物胁迫耐受性转基因植物

    公开(公告)号：US20090133154A1

    公开(公告)日：2009-05-21

    申请号：US11942134

    申请日：2007-11-19

    Applicant: Su-May YU ,  Tuan-Hua David HO

    Inventor： Su-May YU ,  Tuan-Hua David HO

    IPC: A01H5/00 ,  A23L1/10 ,  C12N1/20 ,  C12N15/00 ,  C12N15/11 ,  C12N15/82

    CPC classification number: C12N15/8237 ,  A23L7/10 ,  C12N15/8271 ,  C12N15/8273

    Abstract: In a transgenic plant, a stress-responsive promoter containing CE3 and ABRE2 from HVA1 ABRC3 and CE1 from HVA22 ABRC1, directs low background expression of a gene under normal growth conditions, but is highly inducible by an abiotic stress, such as abscisic acid, dehydration, salt or cold. Compared with the wild type plant, the transgenic plant that expresses a stress-resistant gene under the control of the stress-responsive promoter is more resistant and/or tolerant to abiotic stresses and exhibits similar or higher growth rate and strength under regular or normal environmental conditions.

    Abstract translation: 在转基因植物中，含有来自HVA1ABRC3的CE3和ABRE2的应激响应启动子和来自HVA22ABRC1的CE1在正常生长条件下指导基因的低背景表达，但是由非生物胁迫如脱落酸，脱水高度诱导 ，盐或冷。 与野生型植物相比，在胁迫响应启动子控制下表达抗胁迫基因的转基因植物对非生物胁迫具有更强的抗性和/或耐受性，并且在常规或正常环境下表现出相似或更高的生长速率和强度 条件。

    Sweet potato sporamin gene promoter
    8.
    发明申请
    Sweet potato sporamin gene promoter 有权
    甘薯芽孢基因启动子

    公开(公告)号：US20050198703A1

    公开(公告)日：2005-09-08

    申请号：US10989719

    申请日：2004-11-15

    Applicant: Su-May Yu

    Inventor： Su-May Yu

    IPC: C12N15/82 ,  A01H1/00 ,  A01H5/00 ,  C12N5/04 ,  C12N15/87

    CPC classification number: C12N15/8257 ,  C12N15/8223 ,  C12N15/8225 ,  C12N15/8226

    Abstract: A transformed tuberous plant cell or a transgenic tuberous plant containing a heterologous nucleic acid that includes a sweet potato sporamin promoter operably linked to a sequence encoding a polypeptide.

    Abstract translation: 转化的块茎植物细胞或含有异源核酸的转基因块茎植物，其包含与编码多肽的序列可操作地连接的甘薯孢子淀粉启动子。

    Gene expression system comprising the promoter region of the alpha-amylase genes
    9.
    发明授权
    Gene expression system comprising the promoter region of the alpha-amylase genes 失效
    包含α-淀粉酶基因启动子区的基因表达系统

    公开(公告)号：US5712112A

    公开(公告)日：1998-01-27

    申请号：US343380

    申请日：1994-11-22

    Applicant: Su May Yu ,  Li Fei Liu ,  Ming Tsair Chan

    Inventor： Su May Yu ,  Li Fei Liu ,  Ming Tsair Chan

    IPC: C12N15/29 ,  C12N15/56 ,  C12N15/82 ,  C12N15/84 ,  C12P21/06 ,  C12N5/00 ,  C12N15/00 ,  C12P21/02

    CPC classification number: C12N15/8222 ,  C12N15/8234 ,  C12N15/8238

    Abstract: The present invention is directed to a method for producing a gene product by expressing a gene encoding the gene product in angiosperm host cells, comprising the steps of constructing a vector expressible in angiosperm host cells, the vector comprising a promoter region derived from an .alpha.-amylase gene of a plant, and a gene encoding a desired gene product; transforming a compatible angiosperm host cell with the vector; cultivating the resultant transformant host cell; subjecting the cultivated transformant host cell to a sugar-depleted or sugar-free condition to promote the expression of the gene under the control of the promoter region; and recovering the expressed gene product. The present invention also is directed to a method for the production of a transgenic plant of rice crop comprising the steps of infecting an immature embryo of rice crop with the genus Agrobacterium for transformation; co-culturing the infected embryo with a dicot suspension culture during the step of transformation; allowing the transformed embryo to grow into a callus in a selective medium comprising a sufficient amount of a plant growth hormone for the growth of rice crop; and allowing the cultured callus to regenerate root and shoot in a regeneration medium comprising a pre-determined amount of nutrients for the growth of rice crop. The invention is further directed to a transformed rice plant made by methods of this invention.

    Abstract translation: 本发明涉及通过在被子植物宿主细胞中表达编码基因产物的基因来产生基因产物的方法，包括以下步骤：构建被子植物宿主细胞中可表达的载体，所述载体包含衍生自α- 植物的淀粉酶基因和编码所需基因产物的基因; 用载体转化相容的被子植物宿主细胞; 培养所得转化体宿主细胞; 对培养的转化体宿主细胞进行糖消化或无糖条件以促进基因在启动子区域控制下的表达; 并回收表达的基因产物。 本发明还涉及一种用于生产水稻作物转基因植物的方法，包括用农杆菌感染水稻作物未成熟胚的转化步骤; 在转化步骤期间用双子叶悬浮培养物共同培养感染的胚胎; 使得转化的胚胎在包含足够量的用于水稻作物生长的植物生长激素的选择培养基中生长成愈伤组织; 并且允许培养的愈伤组织在包含用于水稻作物生长的预定量的营养物质的再生培养基中再生根和芽。 本发明进一步涉及通过本发明的方法制备的转化水稻植物。

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