公开(公告)号：US06495322B1

公开(公告)日：2002-12-17

申请号：US09545605

申请日：2000-04-07

申请人： Jian Ni ,  Vishva M. Dixit

发明人： Jian Ni ,  Vishva M. Dixit

IPC分类号： C12Q168

CPC分类号： C12N9/6475 ,  A61K38/00 ,  C07K14/7151 ,  C12N2799/026 ,  G01N33/5008 ,  G01N33/68 ,  G01N2510/00

摘要： The present invention relates to a novel molecule involved in the process of apoptosis. In particular, isolated nucleic acid molecules are provided encoding the human RAIDD protein and splice variants thereof—referred to as RAIDD-SV1 and RAIDD-SV2. RAIDD polypeptides are also provided as are vectors, host cells and recombinant methods for producing the same. The invention further relates to screening methods for identifying agonists and antagonists of RAIDD activity.

摘要翻译： 本发明涉及参与凋亡过程的新型分子。 特别地，提供编码人类RAIDD蛋白及其剪接变体的分离的核酸分子，称为RAIDD-SV1和RAIDD-SV2。 还提供了RAIDD多肽，载体，宿主细胞和用于制备它们的重组方法。 本发明还涉及用于鉴定RAIDD活性的激动剂和拮抗剂的筛选方法。

公开(公告)号：US6060238A

公开(公告)日：2000-05-09

申请号：US591605

申请日：1996-02-09

申请人： Vishva M. Dixit

发明人： Vishva M. Dixit

IPC分类号： A61K38/00 ,  A61K48/00 ,  C07K14/47 ,  C12N15/57 ,  C12Q1/68 ,  C12Q1/37 ,  G01N33/573

CPC分类号： C07K14/4747 ,  A61K38/00 ,  A61K48/00

摘要： This invention provides non-naturally occurring and isolated naturally occurring nucleic acid molecules which encode proteins designated Yama. This invention also provides the polypeptides and proteins encoded by these nucleic acids as well as the purified native polypeptides or proteins. Also provided by this invention is a non-naturally occurring nucleic acid molecule encoding mutant CrmA protein and a dominant inhibitory Yama. Vectors and host cells containing these nucleic acid molecules are further provided. Methods of modulating a cellular function regulated by the Fas receptor pathway in a cell is provided herein. In one aspect, this method comprises introducing into the cell a nucleic acid molecule coding for a gene product having CrmA biological activity such as dominant inhibitory Yama or alternatively, the CrmA gene product. Yama nucleic acid molecules and proteins also can be introduced into the cell to modulate the cellular function regulated by the Fas receptor.

摘要翻译： PCT No.PCT / US96 / 01882 Sec。 371日期1996年2月9日 102（e）日期1996年2月9日PCT提交1996年2月9日PCT公布。 公开号WO96 / 25501 日期1996年8月22日本发明提供非天然存在和分离的天然存在的编码Yama蛋白质的核酸分子。 本发明还提供由这些核酸编码的多肽和蛋白质以及纯化的天然多肽或蛋白质。 本发明还提供编码突变体CrmA蛋白和显性抑制性Yama的非天然存在的核酸分子。 进一步提供含有这些核酸分子的载体和宿主细胞。 本文提供调节由细胞中Fas受体途径调节的细胞功能的方法。 一方面，该方法包括将编码具有CrmA生物学活性的基因产物如显性抑制性Yama或CrmA基因产物的核酸分子引入细胞。 也可以将Yama核酸分子和蛋白质引入细胞中以调节由Fas受体调节的细胞功能。

公开(公告)号：US5688656A

公开(公告)日：1997-11-18

申请号：US441216

申请日：1995-05-15

申请人： Vishva M. Dixit

发明人： Vishva M. Dixit

IPC分类号： C07K16/00 ,  C07K14/00 ,  C07K14/47 ,  C07K16/18 ,  C12N15/09 ,  C12P21/02 ,  C12Q1/68 ,  G01N33/53 ,  G01N33/536 ,  G01N33/543

CPC分类号： C12Q1/6876 ,  C07K14/4718 ,  C07K16/18 ,  C12Q2600/158

摘要： A gene and its gene product which is a marker for inflammatory response have been identified, cloned and sequenced. With the identification of the B61 gene and its product, protein assays of biological fluids as well as nucleic acid probes and antibodies raised to the gene product can be used in a variety of hybridization and immunological assays to detect an impending inflammatory response. Such detection will enable earlier administration of appropriate therapy in immune response diseases.