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    COMPOSITIONS AND METHODS CONTAINING A CONSTITUTIVE PROMOTER TO MODIFY THE EXPRESSION OF GENES OF INTEREST IN PLANTS
    43.
    发明申请
    COMPOSITIONS AND METHODS CONTAINING A CONSTITUTIVE PROMOTER TO MODIFY THE EXPRESSION OF GENES OF INTEREST IN PLANTS 审中-公开
    含有组成促进剂以修改植物感兴趣基因表达的组合物和方法

    公开(公告)号：US20160272982A1

    公开(公告)日：2016-09-22

    申请号：US14778190

    申请日：2014-03-18

    Applicant: EMPRESA BRASILEIRA DE PESQUISA AGROPECUÁRIA - EMBRAPA ,  FUNDAÇÃO UNIVERSIDADE DE BRASILIA - FUB - UNB

    Inventor： Juliana Dantas de Almeida ,  Leila Maria Gomes Barros ,  Renata Henrique Santana ,  Ricardo Vilela Abdelnoor ,  Felipe Rodrigues da Silva

    IPC: C12N15/82

    CPC classification number: C12N15/8216 ,  C12N15/8225 ,  C12N15/8227 ,  C12N15/8234 ,  C12N15/8241

    Abstract: The present invention relates to a new promoter of the expression of genes in plants. More specifically, the invention relates to regulatory sequences of polynucleotides that are isolated from soy plants and can initiate and activate polynucleotide transcription, and to the use of these regulatory sequences to modify the transcription of endogenous and/or heterologous polynucleotides and to produce polypeptides. The invention further describes DNA constructs that contain the promoter of the gene of the supposed flavonol-sulfotransferase protein in soy plants, which promoter is functionally linked to a heterologous and/or endogenous gene. Moreover, the invention relates to the use of these constructs in the form of expression cassettes, expression vectors, recombinant vectors and in plants, plant cells or transgenic protoplasts. The invention also describes a method using such constructs that contain the promoter of the gene of the putative flavonol-sulfotransferase protein of soy plants for the production of transgenic plants, plant cells or protoplasts.

    Abstract translation: 本发明涉及植物中基因表达的新启动子。 更具体地，本发明涉及从大豆植物中分离并可以引发和激活多核苷酸转录的多核苷酸的调节序列，以及这些调节序列用于修饰内源和/或异源多核苷酸的转录并产生多肽的调节序列。 本发明进一步描述了在大豆植物中含有假定的黄酮醇 - 磺基转移酶蛋白基因的启动子的DNA构建体，该启动子与异源和/或内源基因功能性连接。 此外，本发明涉及以表达盒，表达载体，重组载体和植物，植物细胞或转基因原生质体形式的这些构建体的用途。 本发明还描述了一种使用含有大豆植物推定的黄酮醇 - 磺基转移酶蛋白基因的启动子用于生产转基因植物，植物细胞或原生质体的构建体的方法。

    DEVICE FOR MEASURING PRESSURE AND CONTROLLING A FLOW
    45.
    发明申请
    DEVICE FOR MEASURING PRESSURE AND CONTROLLING A FLOW 有权
    用于测量压力和控制流量的装置

    公开(公告)号：US20150059866A1

    公开(公告)日：2015-03-05

    申请号：US14369264

    申请日：2012-12-27

    Applicant: EMPRESA BRASILEIRA DE PESQUISA AGROPECUARIA - EMBRAPA

    Inventor： Adonai Gimenez Calbo

    IPC: A01G25/16 ,  G01D5/02 ,  G05D7/01

    CPC classification number: A01G25/16 ,  A01G7/00 ,  G01D5/02 ,  G01L1/02 ,  G01N3/12 ,  G05D7/0113 ,  Y10T137/1842 ,  Y10T137/2541

    Abstract: The invention relates to a system for measuring cell turgor pressure (dendroturgometer), which can also operate as a firmometer. The system can also be used as an automatic dripper, wherein in this embodiment it is kept pressed by means of a flattening plate (1) against the organ (4), as well as by employing a brace (6) provided with a spring (5) that applies stable force of a magnitude higher than the product between the water pressure, regulated by a pressure adjuster (10) and the fluid layer (2) under the membrane (3), on which the plant exerts compression and obstructs the water flow. The dripping is started when the transpiration provides a drop in the cell turgor pressure of the plant, so that the resulting pressure will be lower than the adjusted water pressure. This system may also be miniaturized and used for measuring the vegetable turgor pressure by using water pressurization with an plunger (11). The cell turgor pressure is achieved with a pressure transducer (12), as the lower pressure applied that forces the passage of water through the layer (2). Alternatively, the system may be mounted for operation as a dendroturgometer in a closed system with a cover (13), and a pressure transducer (12), while a brace applies press force with a spring (5) to enable continuous reading of the turgor pressure in the stern.

    Abstract translation: 本发明涉及一种用于测量细胞膨胀压力（dendrurgurgometer）的系统，其也可以作为计密度计操作。 该系统也可以用作自动滴水器，其中在该实施例中，其通过平板（1）保持压靠在器官（4）上，以及通过使用设有弹簧的支架（6） 5）施加比由压力调节器（10）调节的水压与膜（3）下方的流体层（2）之间的产品的稳定力，该压力调节器（10）在其上施加压缩并阻塞水 流。 当蒸腾作用降低植物的细胞膨胀压力时，开始滴加，使得所得到的压力将低于调节的水压。 该系统也可以小型化并且用于通过使用带有柱塞（11）的水加压来测量蔬菜膨胀压力。 通过压力传感器（12）实现电池膨胀压力，因为施加的较低压力迫使水通过层（2）。 或者，该系统可以安装为在具有盖（13）和压力传感器（12）的封闭系统中作为树状测量计操作，而支架利用弹簧（5）施加压力以使连续读取膨胀器 船尾的压力

    PROTEINS FROM THE WEBS OF NEPHILENGYS CRUENTATA, AVICULARIA JURUENSIS AND PARAWIXIA BISTRIATA SPIDERS ISOLATED FROM BRAZILIAN BIODIVERSITY
    46.
    发明申请
    PROTEINS FROM THE WEBS OF NEPHILENGYS CRUENTATA, AVICULARIA JURUENSIS AND PARAWIXIA BISTRIATA SPIDERS ISOLATED FROM BRAZILIAN BIODIVERSITY 审中-公开
    来自巴西生物多样性分离的NEPHILENGYS CRUENTATA，AVICULARIA JURUENSIS和PARAWIXIA BISTRIATA SPIDERS的蛋白质的蛋白质

    公开(公告)号：US20140206851A1

    公开(公告)日：2014-07-24

    申请号：US13749601

    申请日：2013-01-24

    Applicant: Empresa Brasileira de Pesquisa Agropecuaria - Embrapa ,  Fundacao Universidade de Brasilia

    Inventor： Elibio Leopoldo RECH ,  Natalia Cristina Verza ,  Giovanni Rodrigues Vianna ,  Felipe Rodrigues Da Silva ,  Francisco Jose Lima Aragao ,  Luiz Alberto Colnago ,  Alan Carvalho Andrade ,  Daniela Mantias De Carvalho Bittencourt ,  Pedro Ismael Da Silva Junior ,  Betulia De Morais Souto ,  Luisa de Moraes Madeira ,  Paulo Cesar Motta

    IPC: C07K14/435

    CPC classification number: C07K14/43518 ,  A61K38/00

    Abstract: The Present invention relates to molecules isolated from the nucleic acid that encodes spider web proteins or fragments of these or other derivatives of these. The invention also refers to a chimerical gene and an expression vector containing molecules isolated from the nucleic acid that codes for proteins related to the webs of Nephilengys, Cruentata, Avicularia Juruensis and Parawixia Bistriata spiders. Another embodiment of the present invention are transformed cells containing a chimerical gene or an expression vector of the present invention. Yet another embodiment of the present invention relates to a method for obtaining genetically modified organisms containing inventive chimerical genes or expression vectors and a method for obtaining recombinant proteins from the silks of Nephilengys, Cruentata, Avicularia Juruensis and Parawixia Bistriata spiders. Finally, the invention describes products, such as biofilaments and compositions, using the recombinant proteins of the present invention. The discovery of new spider silk proteins, as well as their characterisation and expression in different heterologous systems shall be of great use in numerous areas, such as medicine and industry.

    Abstract translation: 本发明涉及从编码这些的这些或其他衍生物的蜘蛛网蛋白或其片段的核酸分离的分子。 本发明还涉及嵌合基因和含有从编码与Nephilengys，Cruentata，Avicularia Juruensis和Parawixia Bistriata蜘蛛网相关的蛋白质的核酸分离的分子的表达载体。 本发明的另一个实施方案是含有嵌合基因或本发明的表达载体的转化细胞。 本发明的另一个实施方案涉及一种获得含有本发明嵌合基因或表达载体的转基因生物的方法，以及从Nephilengys，Cruentata，Avicularia Juruensis和Parawixia Bistriata蜘蛛的丝中获得重组蛋白的方法。 最后，本发明描述了使用本发明的重组蛋白质的产物，例如生物丝和组合物。 新型蜘蛛丝蛋白的发现及其在不同异源系统中的表征和表达在医药和工业等领域应用十分广泛。

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