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49 条记录 (耗时 0.058 秒)

    Methods of Producing Peptides in Plants and Peptides Produced Thereby
    41.
    发明申请
    Methods of Producing Peptides in Plants and Peptides Produced Thereby 有权
    在此产生的植物和肽中产生肽的方法

    公开(公告)号：US20110217766A1

    公开(公告)日：2011-09-08

    申请号：US13019819

    申请日：2011-02-02

    申请人： Marcia J. Kieliszewski ,  Jianfeng Xu ,  Gary D. Meyer

    发明人： Marcia J. Kieliszewski ,  Jianfeng Xu ,  Gary D. Meyer

    IPC分类号： C12N15/85

    CPC分类号： C07K14/415 ,  A61K38/27 ,  C07K14/56 ,  C07K14/61 ,  C07K14/765 ,  C12N15/62 ,  C12N15/8257 ,  C12P21/005

    摘要： Methods of increasing the yield in plant expression of recombinant proteins comprising engineering glycosylation sites into cloned genes or cDNAs for proteins using codons that drive post-translational modifications in plants and engineering the cloned genes or cDNAs to contain a plant secretory signal sequence that targets the gene products (protein) for secretion are present. The methods result in increased recombinant glycosylated protein yields. Proteins produced according to these methods are also present.

    摘要翻译： 包括使用驱动植物中翻译后修饰的密码子的蛋白质工程化糖基化位点的重组蛋白质的植物表达产量增加到克隆基因或cDNA的方法，并且设计克隆的基因或cDNA以包含靶向基因的植物分泌信号序列 存在用于分泌的产品（蛋白质）。 该方法导致重组糖基化蛋白质产量增加。 也存在根据这些方法生产的蛋白质。

    Disk drive to reduce head instability
    42.
    发明授权
    Disk drive to reduce head instability 有权
    磁盘驱动器减少头部不稳定

    公开(公告)号：US07974039B1

    公开(公告)日：2011-07-05

    申请号：US12486682

    申请日：2009-06-17

    申请人： Jianfeng Xu ,  Soo-Choon Kang ,  Kazuhiro Saito ,  Hongchao Tim Zhao

    发明人： Jianfeng Xu ,  Soo-Choon Kang ,  Kazuhiro Saito ,  Hongchao Tim Zhao

    IPC分类号： G11B21/02 ,  G11B27/36

    CPC分类号： G11B5/6005 ,  G11B5/6064 ,  G11B5/6076

    摘要： A disk drive to reduce head instability during disk drive manufacturing is disclosed. The disk drive comprises: a disk; a dynamic flying height (DFH) read/write head; a flying height actuator to control the spacing between the DFH read/write head and the disk; and a controller. The controller controls operations in the disk drive including applying a sloped increasing power between a first voltage and a second voltage to move the DFH read/write head towards the disk.

    摘要翻译： 公开了一种在磁盘驱动器制造期间减少磁头不稳定性的磁盘驱动器。 磁盘驱动器包括：磁盘; 动态飞行高度（DFH）读/写头; 用于控制DFH读/写磁头和磁盘之间的间距的飞行高度执行器; 和控制器。 控制器控制磁盘驱动器中的操作，包括在第一电压和第二电压之间施加倾斜的增加功率，以将DFH读/写头朝向磁盘移动。

    METHODS OF PRODUCING PEPTIDES IN PLANTS AND PEPTIDES PRODUCED THEREBY
    47.
    发明申请
    METHODS OF PRODUCING PEPTIDES IN PLANTS AND PEPTIDES PRODUCED THEREBY 审中-公开
    在植物中生产肽的方法和生产的肽

    公开(公告)号：US20100028993A1

    公开(公告)日：2010-02-04

    申请号：US12122606

    申请日：2008-05-16

    申请人： Marcia J. Kieliszewski ,  Jianfeng Xu ,  Gary D. Meyer

    发明人： Marcia J. Kieliszewski ,  Jianfeng Xu ,  Gary D. Meyer

    IPC分类号： C12N15/74

    CPC分类号： C07K14/415 ,  A61K38/27 ,  C07K14/56 ,  C07K14/61 ,  C07K14/765 ,  C12N15/62 ,  C12N15/8257 ,  C12P21/005

    摘要： Methods of increasing the yield in plant expression of recombinant proteins comprising engineering glycosylation sites into cloned genes or cDNAs for proteins using codons that drive post-translational modifications in plants and engineering the cloned genes or cDNAs to contain a plant secretory signal sequence that targets the gene products (protein) for secretion are present. The methods result in increased recombinant glycosylated protein yields. Proteins produced according to these methods are also present.

    摘要翻译： 包括使用驱动植物中翻译后修饰的密码子的蛋白质工程化糖基化位点的重组蛋白质的植物表达产量增加到克隆基因或cDNA的方法，并且设计克隆的基因或cDNA以包含靶向基因的植物分泌信号序列 存在用于分泌的产品（蛋白质）。 该方法导致重组糖基化蛋白质产量增加。 也存在根据这些方法生产的蛋白质。

    Methods of Predicting Hyp-Glycosylation Sites For Proteins Expressed and Secreted in Plant Cells, and Related Methods and Products
    48.
    发明申请
    Methods of Predicting Hyp-Glycosylation Sites For Proteins Expressed and Secreted in Plant Cells, and Related Methods and Products 审中-公开
    预测植物细胞表达和分泌的蛋白质的低糖基化位点的方法及相关方法和产品

    公开(公告)号：US20080242834A1

    公开(公告)日：2008-10-02

    申请号：US11995063

    申请日：2006-07-10

    申请人： Marcia J. Kieliszewski ,  Jianfeng Xu

    发明人： Marcia J. Kieliszewski ,  Jianfeng Xu

    IPC分类号： C07K2/00 ,  C12P21/00

    CPC分类号： C12N15/8257 ,  G16B30/00

    摘要： Proteins with Hyp-glycosylation are more likely to be secreted in plant cells at high levels than those without. Methods are disclosed for the prediction of Pro-hydroxylation and Hyp-glycosylationsites in proteins. Such methods can be used to identify (1) proteins which, without modification, are predisposed to develop Hyp-glycosylation, if expressed in plant cells, and (2) modifications (especially substitution mutations) which increase the propensity of a protein to develop Hyp-glycosylation, with a view to high level or increased secretion. It is also possible to determine empirically whether a particular protein will undergo Hyp-glycosylation suitable for the desired level of secretion in plant cells. Both modified proteins, and methods for the expression and secretion of predisposed and modified proteins, are claimed.

    摘要翻译： 具有低糖基化功能的蛋白质在植物细胞中比不含有高蛋白质的蛋白质更有可能分泌。 公开了用于预测蛋白质中的Pro-羟基化和Hyp-糖基化的方法。 这样的方法可用于鉴定（1）如果在植物细胞中表达，则不经修饰，易于发生Hyp-糖基化的蛋白质，和（2）增加蛋白质发展Hyp的倾向的修饰（特别是取代突变） 糖基化，以达到高水平或增加分泌。 也可以根据经验确定特定蛋白质是否将进行适合植物细胞中所需水平分泌的Hyp糖基化。 要求修饰的蛋白质，以及倾向和修饰的蛋白质的表达和分泌的方法。

    Glycoproteins produced in plants and methods of their use
    49.
    发明申请
    Glycoproteins produced in plants and methods of their use 审中-公开
    在植物中生产的糖蛋白及其使用方法

    公开(公告)号：US20060148680A1

    公开(公告)日：2006-07-06

    申请号：US11036257

    申请日：2005-01-14

    申请人： Marcia Kieliszewski ,  Jianfeng Xu ,  John Kopchick ,  Shigeru Okada

    发明人： Marcia Kieliszewski ,  Jianfeng Xu ,  John Kopchick ,  Shigeru Okada

    IPC分类号： A61K38/27

    CPC分类号： C07K14/415 ,  A61K38/27 ,  C07K14/56 ,  C07K14/61 ,  C07K14/765 ,  C12N15/62 ,  C12N15/8257 ,  C12P21/005

    摘要： Methods of increasing the yield in plant expression of recombinant proteins comprising: engineering glycosylation sites into cloned genes or cDNAs for proteins using codons that drive post-translational modifications in plants; and engineering the cloned genes or cDNAs to contain a plant secretory signal sequence that targets the gene products (protein) for secretion. The methods result in increased recombinant glycosylated protein yields. Proteins produced according to these methods are disclosed.

    摘要翻译： 提高重组蛋白质植物表达产量的方法，其包括：使用驱动植物翻译后修饰的密码子将糖基化位点工程化为克隆基因或蛋白质cDNA; 并对克隆的基因或cDNA进行工程以包含靶向分泌基因产物（蛋白质）的植物分泌信号序列。 该方法导致重组糖基化蛋白质产量增加。 公开了根据这些方法生产的蛋白质。