公开(公告)号：US20220267747A1

公开(公告)日：2022-08-25

申请号：US17741326

申请日：2022-05-10

Applicant: Inscripta, Inc.

Inventor： Aamir Mir ,  Andrew Garst ,  Stephen Federowicz ,  Kyle Seamon

IPC: C12N9/22 ,  C12N15/11 ,  C12N15/70 ,  C12N15/62

Abstract: The present disclosure relates to methods and compositions that allow one to identify in vivo edited cells when employing nucleic-acid guided editing. Additionally provided are automated multi-module instruments for performing editing and selection methods and using the compositions.

公开(公告)号：US20220177865A1

公开(公告)日：2022-06-09

申请号：US17680279

申请日：2022-02-24

Applicant: Inscripta, Inc.

Inventor： Aamir Mir ,  Andrew Garst ,  Stephen Federowicz ,  Kyle Seamon

IPC: C12N9/22 ,  C12N15/11 ,  C12N15/70 ,  C12N15/62

Abstract: The present disclosure relates to methods and compositions that allow one to identify in vivo edited cells when employing nucleic-acid guided editing. Additionally provided are automated multi-module instruments for performing editing and selection methods and using the compositions.

公开(公告)号：US20220145276A1

公开(公告)日：2022-05-12

申请号：US17521739

申请日：2021-11-08

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Christian Siltanen

IPC: C12N9/22 ,  C12N15/11 ,  C12N15/90

Abstract: The present disclosure relates to automated multi-module instruments, compositions and methods for performing nucleic acid-guided nuclease editing; specifically, the disclosure provides nucleic acid cassettes, plasmids, vectors, and compositions comprising the same that employ homologous recombination for genome engineering by having a CRISPR nuclease cause a specific DSB while tethered to a repair nucleic acid.

公开(公告)号：US20220073892A1

公开(公告)日：2022-03-10

申请号：US17526951

申请日：2021-11-15

Applicant: Inscripta, Inc.

Inventor： Aamir Mir ,  Andrew Garst ,  Stephen Federowicz ,  Kyle Seamon

IPC: C12N9/22 ,  C12N15/11 ,  C12N15/70 ,  C12N15/62

Abstract: The present disclosure relates to methods and compositions that allow one to identify in vivo edited cells when employing nucleic-acid guided editing. Additionally provided are automated multi-module instruments for performing editing and selection methods and using the compositions.

公开(公告)号：US11130970B2

公开(公告)日：2021-09-28

申请号：US17179193

申请日：2021-02-18

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Ryan T. Gill ,  Tanya Elizabeth Warnecke Lipscomb

IPC: C12N15/90 ,  C12N15/70 ,  C12N15/81 ,  C12N15/85 ,  C12N9/22 ,  C12N15/11

Abstract: Disclosed herein are nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Disclosed herein are engineered non-naturally occurring nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Targetable nuclease systems can be used to edit genetic targets, including recursive genetic engineering and trackable genetic engineering methods.

公开(公告)号：US11085030B2

公开(公告)日：2021-08-10

申请号：US17200074

申请日：2021-03-12

Applicant: Inscripta, Inc.

Inventor： Benjamin Mijts ,  Juhan Kim ,  Aamir Mir ,  Andrew Garst ,  Kyle Seamon

IPC: C12N9/22 ,  C12N15/113

Abstract: The present disclosure provides new RNA-guided nucleases for making rational, direct edits to nucleic acids in live cells.

公开(公告)号：US11072774B2

公开(公告)日：2021-07-27

申请号：US17175612

申请日：2021-02-13

Applicant: Inscripta, Inc.

Inventor： Phillip Belgrader ,  Don Masquelier ,  Andrew Garst ,  Richard Fox

IPC: C12M1/00 ,  C12M1/26 ,  C12N15/87 ,  C12M3/00 ,  B01D63/08 ,  B01D69/06 ,  B01D71/02

Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.

公开(公告)号：US20200230556A1

公开(公告)日：2020-07-23

申请号：US16844330

申请日：2020-04-09

Applicant: Inscripta, Inc.

Inventor： Phillip Belgrader ,  Don Masquelier ,  Matthew Estes ,  Bruce Chabansky ,  Andrew Garst ,  Richard Fox ,  Jorge Bernate

IPC: B01D63/08

Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.

公开(公告)号：US10647958B2

公开(公告)日：2020-05-12

申请号：US16693630

申请日：2019-11-25

Applicant: Inscripta, Inc.

Inventor： Phillip Belgrader ,  Don Masquelier ,  Bruce Chabansky ,  Jorge Bernate ,  Andrew Garst ,  Richard Fox

IPC: C12M1/00 ,  C12M3/00 ,  C12M1/26 ,  C12N15/87 ,  B01D63/08 ,  B01D69/06 ,  B01D71/02

Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.

公开(公告)号：US20200056143A1

公开(公告)日：2020-02-20

申请号：US16454865

申请日：2019-06-27

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Richard Fox ,  Phillip Belgrader ,  Don Masquelier

IPC: C12M1/00 ,  C12M3/00 ,  C12N15/87 ,  C12M1/26

Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.