公开(公告)号：US11667932B2

公开(公告)日：2023-06-06

申请号：US17523821

申请日：2021-11-10

Applicant: Inscripta, Inc.

Inventor： Christian Siltanen ,  Megan Basila

IPC: C12N15/87 ,  C12N13/00

CPC classification number: C12N15/87 ,  C12N13/00

Abstract: The present disclosure provides a sphere-packing lattice electroporation device configured for use as a stand-alone unit or in an automated multi-module cell processing environment and configured to decrease cell processing time and cell survival. The sphere-packing lattice utilizes lattice-forming beads that are uniform in size and that self-assemble into a crystalline-like lattice.

公开(公告)号：US20230250420A1

公开(公告)日：2023-08-10

申请号：US18174397

申请日：2023-02-24

Applicant: Inscripta, Inc.

Inventor： Phillip Belgrader ,  Nathan Bade ,  Christian Siltanen ,  Aamir Mir ,  Xi-Jun Chen ,  Janine Mok ,  Burak Dura ,  Bruce Chabansky ,  David Stumbo ,  Eric Smith ,  Jorge Bernate

IPC: C12N15/10 ,  C12M1/34 ,  C12M1/26 ,  C12N15/113 ,  C12N15/86 ,  C12M3/00 ,  C12M3/04 ,  C12M1/00 ,  C12N5/074 ,  C12N15/11 ,  C12N15/88 ,  C12N15/90 ,  B01L3/00 ,  B01L7/00 ,  C12M1/32 ,  C12M3/06

CPC classification number: C12N15/1082 ,  C12M41/36 ,  C12M33/14 ,  C12N15/113 ,  C12N15/86 ,  C12M23/42 ,  C12M27/10 ,  C12M29/04 ,  C12M43/00 ,  C12M47/02 ,  C12N5/0696 ,  C12N15/1065 ,  C12N15/1068 ,  C12N15/11 ,  C12N15/88 ,  C12N15/907 ,  B01L3/502761 ,  B01L7/00 ,  C12M23/12 ,  C12M23/16 ,  C12M23/50 ,  C12M47/04 ,  C12N9/22

Abstract: Compositions of matter, methods, modules, and automated instruments may relate to synthesizing a library including an editing cassette including a different gRNA and donor DNA pair, amplifying the editing cassette in a partition separate from other editing cassettes in the library, adding nuclease to the partition, and adding lipofectamine to the editing cassette and nuclease to form a lipofectamine/nucleic acid/nuclease complex. A microcarrier coated in extracellular matrix or a cell adhesion molecule coating may be added to the lipofectamine/nucleic acid/nuclease complex. Cell growth material, the microcarrier, and mammalian cells may be transferred to a growth module in an automated closed cell editing instrument via a liquid handling system. The mammalian cells may be allowed to seed on the microcarrier. Conditions may be provided for the mammalian cells to take-up and be edited by a payload associated with the lipofectamine/nucleic acid/nuclease complex. The mammalian cells may be detached from the microcarrier.

公开(公告)号：US20220145327A1

公开(公告)日：2022-05-12

申请号：US17584298

申请日：2022-01-25

Applicant: Inscripta, Inc.

Inventor： Phillip Belgrader ,  Christian Siltanen ,  William Watterson ,  Burak Dura ,  Bruce Chabansky ,  David Stumbo ,  Eric Smith ,  Jorge Bernate

IPC: C12N15/86 ,  C12N9/22 ,  C12N15/11 ,  C12N5/074 ,  C12N5/071 ,  C12M1/36 ,  C12M1/12 ,  C12M1/06 ,  C12M1/02 ,  C12M1/34 ,  C12M1/00 ,  C12M1/26

Abstract: This invention relates to compositions of matter, methods, modules and instruments for automated mammalian cell growth and mammalian cell transduction followed by nucleic acid-guided nuclease editing in live mammalian cells.

公开(公告)号：US20250043314A1

公开(公告)日：2025-02-06

申请号：US18717586

申请日：2022-12-19

Applicant: Inscripta, Inc.

Inventor： Erik Zimmerman ,  Emily Feldman ,  Christian Siltanen ,  Marina Barreto Felisbino

IPC: C12N15/90 ,  C12N9/12 ,  C12N9/22 ,  C12N15/11 ,  C12Q1/6869

Abstract: The present disclosure provides compositions of matter, methods and instruments for nucleic acid-guided nickase/reverse transcriptase fusion enzyme editing of nucleic acids in live mammalian cells, and for tracking of editing events.

公开(公告)号：US20220307014A1

公开(公告)日：2022-09-29

申请号：US17829263

申请日：2022-05-31

Applicant: Inscripta, Inc.

Inventor： Burak Dura ,  Phillip Belgrader ,  Christian Siltanen ,  William Watterson ,  Bruce Chabansky ,  David Stumbo ,  Eric Smith ,  Jorge Bernate

IPC: C12N15/10 ,  C12M1/34 ,  C12M1/26 ,  C12N15/113 ,  C12N15/86 ,  C12M3/00 ,  C12M3/04 ,  C12M1/00 ,  C12N5/074 ,  C12N15/11 ,  C12N15/88 ,  C12N15/90 ,  B01L3/00 ,  B01L7/00 ,  C12M1/32 ,  C12M3/06

Abstract: This invention relates to compositions of matter, methods, modules and instruments for automated mammalian cell growth and mammalian cell transduction followed by nucleic acid-guided nuclease editing in live mammalian cells.

公开(公告)号：US20220145276A1

公开(公告)日：2022-05-12

申请号：US17521739

申请日：2021-11-08

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Christian Siltanen

IPC: C12N9/22 ,  C12N15/11 ,  C12N15/90

Abstract: The present disclosure relates to automated multi-module instruments, compositions and methods for performing nucleic acid-guided nuclease editing; specifically, the disclosure provides nucleic acid cassettes, plasmids, vectors, and compositions comprising the same that employ homologous recombination for genome engineering by having a CRISPR nuclease cause a specific DSB while tethered to a repair nucleic acid.

公开(公告)号：US20220073949A1

公开(公告)日：2022-03-10

申请号：US17523821

申请日：2021-11-10

Applicant: Inscripta, Inc.

Inventor： Christian Siltanen ,  Megan Basila

IPC: C12N15/87

Abstract: The present disclosure provides a sphere-packing lattice electroporation device configured for use as a stand-alone unit or in an automated multi-module cell processing environment and configured to decrease cell processing time and cell survival. The sphere-packing lattice utilizes lattice-forming beads that are uniform in size and that self-assemble into a crystalline-like lattice.

公开(公告)号：US11225674B2

公开(公告)日：2022-01-18

申请号：US17158488

申请日：2021-01-26

Applicant: Inscripta, Inc.

Inventor： Christian Siltanen ,  Megan Basila

IPC: C12N15/87

Abstract: The present disclosure provides a sphere-packing lattice electroporation device configured for use as a stand-alone unit or in an automated multi-module cell processing environment and configured to decrease cell processing time and increase cell survival. The sphere-packing lattice utilizes lattice-forming beads that are uniform in size and that self-assemble into a crystalline-like lattice.

公开(公告)号：US20210230635A1

公开(公告)日：2021-07-29

申请号：US17158488

申请日：2021-01-26

Applicant: Inscripta, Inc.

Inventor： Christian Siltanen ,  Megan Basila

IPC: C12N15/87

Abstract: The present disclosure provides a sphere-packing lattice electroporation device configured for use as a stand-alone unit or in an automated multi-module cell processing environment and configured to decrease cell processing time and cell survival.

公开(公告)号：US20230174961A1

公开(公告)日：2023-06-08

申请号：US18101543

申请日：2023-01-25

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Christian Siltanen

IPC: C12N9/22 ,  C12N15/90 ,  C12N15/11

CPC classification number: C12N9/22 ,  C12N15/11 ,  C12N15/902 ,  C12N2310/20

Abstract: The present disclosure relates to automated multi-module instruments, compositions and methods for performing nucleic acid-guided nuclease editing; specifically, the disclosure provides nucleic acid cassettes, plasmids, vectors, and compositions comprising the same that employ homologous recombination for genome engineering by having a CRISPR nuclease cause a specific DSB while tethered to a repair nucleic acid.