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    NUCLEOTIDE SEQUENCES MEDIATING MALE FERTILITY AND METHOD OF USING SAME
    43.
    发明申请
    NUCLEOTIDE SEQUENCES MEDIATING MALE FERTILITY AND METHOD OF USING SAME 审中-公开
    核苷酸序列介导雄性不育及其使用方法

    公开(公告)号：US20080134362A1

    公开(公告)日：2008-06-05

    申请号：US11968557

    申请日：2008-01-02

    申请人： Marc C. Albertsen ,  Tim Fox ,  Gary Huffman ,  Mary Trimnell

    发明人： Marc C. Albertsen ,  Tim Fox ,  Gary Huffman ,  Mary Trimnell

    IPC分类号： C12N15/87 ,  A01H1/00

    CPC分类号： C12N15/8289 ,  C07K14/415 ,  C12N15/8217 ,  C12N15/8222 ,  C12N15/8231

    摘要： Nucleotide sequences mediating male fertility in plants are described, with DNA molecule and amino acid sequences set forth. The nucleotide sequences are useful in mediating male fertility in plants. In an embodiment the sequence in the plant is inactivated, and a second sequence linked to an inducible promoter introduced into a plant, such that the plant is constitutively male sterile. Fertility is induced by exposing the plant to an inducing substance.

    摘要翻译： 描述介导植物中雄性生育力的核苷酸序列，其中阐述了DNA分子和氨基酸序列。 核苷酸序列可用于介导植物中的雄性生殖力。 在一个实施方案中，植物中的序列是灭活的，并且连接到引入植物的诱导型启动子的第二序列，使得该植物是组成型雄性不育的。 通过将植物暴露于诱导物质来诱导生育力。

    METHODS FOR LARGE SCALE FUNCTIONAL EVALUATION OF NUCLEOTIDE SEQUENCES IN PLANTS
    49.
    发明申请
    METHODS FOR LARGE SCALE FUNCTIONAL EVALUATION OF NUCLEOTIDE SEQUENCES IN PLANTS 审中-公开
    植物核酸序列大规模功能评估方法

    公开(公告)号：US20120329674A1

    公开(公告)日：2012-12-27

    申请号：US13312032

    申请日：2011-12-06

    申请人： Marc C. Albertsen ,  Susan J. Martino-Catt ,  Xiaomu Niu ,  Igor Cunha Oliveira ,  Dwight Tomes ,  Haiyin Wang ,  Deping Xu

    发明人： Marc C. Albertsen ,  Susan J. Martino-Catt ,  Xiaomu Niu ,  Igor Cunha Oliveira ,  Dwight Tomes ,  Haiyin Wang ,  Deping Xu

    IPC分类号： C40B30/06

    CPC分类号： C12N15/1082

    摘要： The present invention provides for rapid and large scale evaluation of expression of, or function of, nucleotide sequences in plants. The invention comprises three specific components which provide for fast and large scale evaluation of nucleotide sequences. The first component includes delivery in either a single event a library of different engineered vectors or a single engineered vector for a single target nucleotide sequence comprising sequences the function of which is desired to be known in plant cells. Surprisingly, applicants have discovered that, the introduction of multiple vectors to plant cells predominantly results in individual transgenic plants which contain only a single transformation event. The second feature of the invention involves a highly transformable, fast cycling and/or miniature size plant and the final step involves mass scale analysis of T0 plants for various phenotypes and plasmid rescue to identify the nucleotide sequence present in a particular phenotype.

    摘要翻译： 本发明提供对植物中核苷酸序列的表达或功能的快速和大规模评估。 本发明包括提供快速和大规模评估核苷酸序列的三个特定组分。 第一组分包括在单一事件中递送不同工程化载体的文库或单个靶核苷酸序列的单一工程载体，其包含其功能期望在植物细胞中已知的序列。 令人惊讶的是，申请人已经发现，将多个载体引入植物细胞主要导致单个转基因植物，其仅包含单个转化事件。 本发明的第二个特征涉及高度可变形，快速循环和/或微型尺寸的植物，并且最终步骤涉及用于各种表型和质粒拯救的T0植物的大规模分析，以鉴定特定表型中存在的核苷酸序列。