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    Methods and compositions for modulating flowering and maturity in plants
    1.
    发明授权
    Methods and compositions for modulating flowering and maturity in plants 有权
    用于调节植物开花和成熟的方法和组合物

    公开(公告)号：US08173867B2

    公开(公告)日：2012-05-08

    申请号：US12276961

    申请日：2008-11-24

    Applicant: Dwight T. Tomes ,  Silvio Salvi ,  Michele Morgante ,  Giorgio Sponza ,  Edward Bruggemann ,  Xiaomu Niu ,  Bailin Li ,  Roberto Tuberosa

    Inventor： Dwight T. Tomes ,  Silvio Salvi ,  Michele Morgante ,  Giorgio Sponza ,  Edward Bruggemann ,  Xiaomu Niu ,  Bailin Li ,  Roberto Tuberosa

    IPC: C12N15/82

    CPC classification number: C12N15/827 ,  C07K14/415

    Abstract: The present invention provides compositions and methods for modulating flowering time in plants. Maize RAP2.7 nucleotide sequences are disclosed which upon overexpression cause later flowering and when inhibited cause earlier flowering. Also disclosed is a DNA sequence which acts as a regulator/enhancer of RAP2.7, termed VGT1. This sequence does not code for any known protein, but acts as either a RNAi element or a regulatory DNA or RNA element that either directly regulates expression of flowering genes such as Rap2.7 or specifically targets expression of other genes which control flowering genes such as Rap2.7. This element this can be used as a sequence-based marker to identify inbred and hybrids which have altered maturity. Methods for expressing these nucleotide sequences in a plant for modifying maturity and flowering in plants are provided as well as expression constructs, vectors, transformed cells and plants.

    Abstract translation: 本发明提供调节植物开花时间的组合物和方法。 公开了玉米RAP2.7核苷酸序列，其在过表达导致后期开花并且当被抑制时引起早期开花。 还公开了作为RAP2.7调节/增强子的DNA序列，称为VGT1。 该序列不编码任何已知的蛋白质，而是作为直接调节开花基因如Rap2.7的表达的RNAi元件或调节DNA或RNA元件，或者特异性靶向控制开花基因的其他基因的表达，例如 Rap2.7。 这个元素可以用作基于序列的标记来鉴定已经改变成熟度的近交系和杂交种。 提供用于在植物中表达这些核苷酸序列以修饰植物中的成熟和开花的方法以及表达构建体，载体，转化的细胞和植物。

    Methods of identifying a plant with an Nsf1 locus
    2.
    发明授权
    Methods of identifying a plant with an Nsf1 locus 有权
    鉴定具有Nsf1基因座的植物的方法

    公开(公告)号：US07838263B2

    公开(公告)日：2010-11-23

    申请号：US11964871

    申请日：2007-12-27

    Applicant: Thao Dam ,  Anthony D. Guida, Jr. ,  Christine B. Hazel ,  Bailin Li ,  Mark E. Williams

    Inventor： Thao Dam ,  Anthony D. Guida, Jr. ,  Christine B. Hazel ,  Bailin Li ,  Mark E. Williams

    IPC: C12Q1/26 ,  C12Q1/68

    CPC classification number: C12N15/8278 ,  C12N15/8274

    Abstract: This invention relates to polynucleotide sequences encoding a gene that can confer resistance to at least one herbicide. It further relates to plants and seeds of plants carrying chimeric genes comprising said polynucleotide sequences, which enhance or confer resistance to at least one herbicide, and methods of making said plants and seeds. The invention further presents sequences that can be used as molecular markers that in turn can be used to identify the region of interest in corn lines resulting from new crosses and to quickly and efficiently select the best lines for breeding strategies by avoiding sensitive lines. Methods of identifying a plant with an Nsf1 locus using molecular markers are described.

    Abstract translation: 本发明涉及编码可赋予至少一种除草剂抗性的基因的多核苷酸序列。 它进一步涉及携带包含所述多核苷酸序列的嵌合基因的植物和种子，其增强或赋予至少一种除草剂的抗性，以及制备所述植物和种子的方法。 本发明还提供可用作分子标记的序列，其又可用于鉴定由新杂交产生的玉米品系中的感兴趣区域，并通过避免敏感品系快速有效地选择育种策略的最佳品系。 描述了使用分子标记鉴定具有Nsf1基因座的植物的方法。

    Delayed flowering time gene (DLF1) in maize and uses thereof
    3.
    发明授权
    Delayed flowering time gene (DLF1) in maize and uses thereof 失效
    玉米延迟开花时间基因（DLF1）及其用途

    公开(公告)号：US07763778B2

    公开(公告)日：2010-07-27

    申请号：US11624807

    申请日：2007-01-19

    Applicant: Olga Danilevskaya ,  Michael Muszynski ,  Bailin Li ,  Thao Dam

    Inventor： Olga Danilevskaya ,  Michael Muszynski ,  Bailin Li ,  Thao Dam

    IPC: C12N15/29 ,  C12N15/82 ,  C12N15/87 ,  A01H5/00 ,  A01H5/10

    CPC classification number: C07K14/415 ,  C12N15/827

    Abstract: An isolated polynucleotide encoding a delayed flowering gene (DLF1) is provided, as is the isolated DLF1 protein, peptide portions thereof, and functional fragments of the DLF1. Also provided is a transgenic plant, which contains in its genome a transgene containing the polynucleotide encoding the DLF1 protein, or a nucleotide sequence complementary to the encoding polynucleotide. In addition, methods of identifying a nucleotide sequence and/or a heterologous protein that is specifically bound by the DLF1 protein, methods of using the isolated polynucleotide (or encoded polypeptide), for example, to modulate plant cell growth and/or development, are provided.

    Abstract translation: 提供编码延迟开花基因（DLF1）的分离的多核苷酸，分离的DLF1蛋白，其肽部分和DLF1的功能片段。 还提供了一种转基因植物，其在其基因组中含有含有编码DLF1蛋白的多核苷酸或与编码多核苷酸互补的核苷酸序列的转基因。 此外，识别由DLF1蛋白特异性结合的核苷酸序列和/或异源蛋白的方法，使用分离的多核苷酸（或编码的多肽）例如调节植物细胞生长和/或发育的方法是 提供。

    Polynucleotide encoding a maize herbicide resistance gene and methods for use
    4.
    发明授权
    Polynucleotide encoding a maize herbicide resistance gene and methods for use 有权
    编码玉米除草剂抗性基因的多核苷酸及其使用方法

    公开(公告)号：US07705200B2

    公开(公告)日：2010-04-27

    申请号：US12428648

    申请日：2009-04-23

    Applicant: Thao Dam ,  Anthony D. Guida, Jr. ,  Christine B. Hazel ,  Bailin Li ,  Mark E. Williams

    Inventor： Thao Dam ,  Anthony D. Guida, Jr. ,  Christine B. Hazel ,  Bailin Li ,  Mark E. Williams

    IPC: C12N15/82 ,  A01H5/00 ,  A01N25/00

    CPC classification number: C12N15/8278 ,  C12N15/8274

    Abstract: This invention relates to polynucleotide sequences encoding a gene that can confer resistance to at least one herbicide. It further relates to plants and seeds of plants carrying chimeric genes comprising said polynucleotide sequences, which enhance or confer resistance to at least one herbicide, and methods of making said plants and seeds. The invention further presents sequences that can be used as molecular markers that in turn can be used to identify the region of interest in corn lines resulting from new crosses and to quickly and efficiently select the best lines for breeding strategies by avoiding sensitive lines.

    Abstract translation: 本发明涉及编码可赋予至少一种除草剂抗性的基因的多核苷酸序列。 它进一步涉及携带包含所述多核苷酸序列的嵌合基因的植物和种子，其增强或赋予至少一种除草剂的抗性，以及制备所述植物和种子的方法。 本发明还提供可用作分子标记的序列，其又可用于鉴定由新杂交产生的玉米品系中的感兴趣区域，并通过避免敏感品系快速有效地选择育种策略的最佳品系。

    Polynucleotide Encoding a Maize Herbicide Resistance Gene and Methods for Use
    7.
    发明申请
    Polynucleotide Encoding a Maize Herbicide Resistance Gene and Methods for Use 审中-公开
    编码玉米除草剂抗性基因的多核苷酸和使用方法

    公开(公告)号：US20080050818A1

    公开(公告)日：2008-02-28

    申请号：US11926168

    申请日：2007-10-29

    Applicant: Thao Dam ,  Anthony Guida ,  Christine Hazel ,  Bailin Li ,  Mark Williams

    Inventor： Thao Dam ,  Anthony Guida ,  Christine Hazel ,  Bailin Li ,  Mark Williams

    IPC: A01H5/00 ,  C07H21/04 ,  C12N15/00

    CPC classification number: C12N15/8278 ,  C12N15/8274

    Abstract: This invention relates to polynucleotide sequences encoding a gene that can confer resistance to at least one herbicide. It further relates to plants and seeds of plants carrying chimeric genes comprising said polynucleotide sequences, which enhance or confer resistance to at least one herbicide, and methods of making said plants and seeds. The invention further presents sequences that can be used as molecular markers that in turn can be used to identify the region of interest in corn lines resulting from new crosses and to quickly and efficiently select the best lines for breeding strategies by avoiding sensitive lines.

    Abstract translation: 本发明涉及编码可赋予至少一种除草剂抗性的基因的多核苷酸序列。 它进一步涉及携带包含所述多核苷酸序列的嵌合基因的植物和种子，其增强或赋予至少一种除草剂的抗性，以及制备所述植物和种子的方法。 本发明还提供可用作分子标记的序列，其又可用于鉴定由新杂交产生的玉米品系中的感兴趣区域，并通过避免敏感品系快速有效地选择育种策略的最佳品系。

    Delayed Flowering Time Gene (DLF1) in Maize and Uses Thereof
    8.
    发明申请
    Delayed Flowering Time Gene (DLF1) in Maize and Uses Thereof 失效
    延迟开花时间基因（DLF1）在玉米及其用途

    公开(公告)号：US20070256198A1

    公开(公告)日：2007-11-01

    申请号：US11624807

    申请日：2007-01-19

    Applicant: Olga Danilevskaya ,  Michael Muszynski ,  Bailin Li ,  Thao Dam

    Inventor： Olga Danilevskaya ,  Michael Muszynski ,  Bailin Li ,  Thao Dam

    IPC: A01H5/00 ,  A01H1/00 ,  A01H5/10 ,  C12N15/00 ,  C12N5/04 ,  C12N15/82 ,  C07H21/04 ,  A01H3/00

    CPC classification number: C07K14/415 ,  C12N15/827

    Abstract: An isolated polynucleotide encoding a delayed flowering gene (DLF1) is provided, as is the isolated DLF1 protein, peptide portions thereof, and functional fragments of the DLF1. Also provided is a transgenic plant, which contains in its genome a transgene containing the polynucleotide encoding the DLF1 protein, or a nucleotide sequence complementary to the encoding polynucleotide. In addition, methods of identifying a nucleotide sequence and/or a heterologous protein that is specifically bound by the DLF1 protein, methods of using the isolated polynucleotide (or encoded polypeptide), for example, to modulate plant cell growth and/or development, are provided.

    Abstract translation: 提供编码延迟开花基因（DLF1）的分离的多核苷酸，分离的DLF1蛋白，其肽部分和DLF1的功能片段。 还提供了一种转基因植物，其在其基因组中含有含有编码DLF1蛋白的多核苷酸或与编码多核苷酸互补的核苷酸序列的转基因。 此外，识别由DLF1蛋白特异性结合的核苷酸序列和/或异源蛋白的方法，使用分离的多核苷酸（或编码的多肽）例如调节植物细胞生长和/或发育的方法是 提供。

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