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    METHODS FOR LARGE SCALE FUNCTIONAL EVALUATION OF NUCLEOTIDE SEQUENCES IN PLANTS
    1.
    发明申请
    METHODS FOR LARGE SCALE FUNCTIONAL EVALUATION OF NUCLEOTIDE SEQUENCES IN PLANTS 审中-公开
    植物核酸序列大规模功能评估方法

    公开(公告)号：US20120329674A1

    公开(公告)日：2012-12-27

    申请号：US13312032

    申请日：2011-12-06

    申请人： Marc C. Albertsen ,  Susan J. Martino-Catt ,  Xiaomu Niu ,  Igor Cunha Oliveira ,  Dwight Tomes ,  Haiyin Wang ,  Deping Xu

    发明人： Marc C. Albertsen ,  Susan J. Martino-Catt ,  Xiaomu Niu ,  Igor Cunha Oliveira ,  Dwight Tomes ,  Haiyin Wang ,  Deping Xu

    IPC分类号： C40B30/06

    CPC分类号： C12N15/1082

    摘要： The present invention provides for rapid and large scale evaluation of expression of, or function of, nucleotide sequences in plants. The invention comprises three specific components which provide for fast and large scale evaluation of nucleotide sequences. The first component includes delivery in either a single event a library of different engineered vectors or a single engineered vector for a single target nucleotide sequence comprising sequences the function of which is desired to be known in plant cells. Surprisingly, applicants have discovered that, the introduction of multiple vectors to plant cells predominantly results in individual transgenic plants which contain only a single transformation event. The second feature of the invention involves a highly transformable, fast cycling and/or miniature size plant and the final step involves mass scale analysis of T0 plants for various phenotypes and plasmid rescue to identify the nucleotide sequence present in a particular phenotype.

    摘要翻译： 本发明提供对植物中核苷酸序列的表达或功能的快速和大规模评估。 本发明包括提供快速和大规模评估核苷酸序列的三个特定组分。 第一组分包括在单一事件中递送不同工程化载体的文库或单个靶核苷酸序列的单一工程载体，其包含其功能期望在植物细胞中已知的序列。 令人惊讶的是，申请人已经发现，将多个载体引入植物细胞主要导致单个转基因植物，其仅包含单个转化事件。 本发明的第二个特征涉及高度可变形，快速循环和/或微型尺寸的植物，并且最终步骤涉及用于各种表型和质粒拯救的T0植物的大规模分析，以鉴定特定表型中存在的核苷酸序列。

    Reversible nuclear genetic system for male sterility in transgenic plants
    6.
    发明授权
    Reversible nuclear genetic system for male sterility in transgenic plants 有权
    在转基因植物中雄性不育的可逆核遗传系统

    公开(公告)号：US06399856B1

    公开(公告)日：2002-06-04

    申请号：US09195716

    申请日：1998-11-19

    申请人： Andrew M. Cigan ,  Marc C. Albertsen

    发明人： Andrew M. Cigan ,  Marc C. Albertsen

    IPC分类号： A01H100

    CPC分类号： C12N9/1007 ,  C07K14/415 ,  C12N15/8216 ,  C12N15/8231 ,  C12N15/8289 ,  Y10S47/01

    摘要： Plant development can be altered by transforming a plant with a genetic construct that includes regulatory elements and DNA sequences capable of acting in a fashion to inhibit pollen formation or function, thus rendering the transformed plant reversibly male-sterile. In particular, the present invention relates to the use of dominant negative genes and an anther-specific promoter. Male sterility is reversed by incorporation into a plant of a second genetic construct which represses the dominant negative gene. The invention also relates to novel DNA sequences which exhibit the ability to serve as anther-specific promoters in plants.

    摘要翻译： 可以通过用包括调节元件和能够以抑制花粉形成或功能的方式起作用的DNA序列的遗传构建体转化植物来改变植物发育，从而使转化的植物可逆地雄性不育。 特别地，本发明涉及显性阴性基因和花药特异性启动子的用途。 通过掺入抑制显性阴性基因的第二遗传构建体的植物中，雄性不育是逆转的。 本发明还涉及在植物中表现出作为花药特异性启动子的能力的新型DNA序列。

    Transgenic plants and DNA comprising anther specific promoter 5126 and gene to achieve male sterility
    8.
    发明授权
    Transgenic plants and DNA comprising anther specific promoter 5126 and gene to achieve male sterility 失效
    转基因植物和包含花药特异性启动子5126和基因的DNA以实现雄性不育

    公开(公告)号：US5689051A

    公开(公告)日：1997-11-18

    申请号：US474556

    申请日：1995-06-07

    申请人： Andrew M. Cigan ,  Marc C. Albertsen

    发明人： Andrew M. Cigan ,  Marc C. Albertsen

    IPC分类号： A01H1/02 ,  C07K14/415 ,  C12N9/10 ,  C12N15/82 ,  C12N15/00 ,  A01H1/06 ,  A01H4/00 ,  C12N15/05

    CPC分类号： C12N15/8289 ,  C07K14/415 ,  C12N15/8216 ,  C12N15/8231 ,  C12N9/1007 ,  Y10S47/01

    摘要： Plant development can be altered by transforming a plant with a genetic construct that includes regulatory elements and DNA sequences capable of acting in a fashion to inhibit pollen formation or function, thus rendering the transformed plant male-sterile. In particular, the present invention relates to an isolated DNA molecule comprising an anther-specific promoter, designated 5126, its operable linkage to a DNA sequence encoding a gene product and transgenic plants comprising said DNA. More particularly the gene product linked to promoter 5126 disrupts the function or formation of pollen.

    摘要翻译： 可以通过用包括调节元件和能够以抑制花粉形成或功能的方式起作用的DNA序列的遗传构建体转化植物来改变植物发育，从而使转化的植物雄性不育。 特别地，本发明涉及分离的DNA分子，其包含命名为5126的花药特异性启动子，其与编码基因产物的DNA序列的可操作连接以及包含所述DNA的转基因植物。 更特别地，与启动子5126连接的基因产物破坏了花粉的功能或形成。