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公开(公告)号：US07205455B2
公开(公告)日：2007-04-17
申请号：US10295370
申请日：2002-11-15
CPC分类号： C12N15/8287 , C07K14/43577 , C07K14/721 , C07K2319/00
摘要： The field of the invention is inducible gene expression systems wherein expression is controlled by a novel ecdysone or ultraspiracle receptor or its derivatives. The field particularly relates to the isolation and characterization of nucleic acid and polypeptides for a novel ecdysone receptor and a novel ultraspiracle receptor. The nucleic acid and polypeptides are useful in novel gene expression systems inducible with ecdysone or derivative receptor agonists.
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公开(公告)号：US07151168B2
公开(公告)日：2006-12-19
申请号：US10292324
申请日：2002-11-12
CPC分类号： C12N15/8287 , C07K14/43577 , C07K14/721 , C07K2319/00
摘要： The field of the invention is inducible gene expression systems wherein expression is controlled by a novel ecdysone or ultraspiracle receptor or its derivatives. The field particularly relates to the isolation and characterization of nucleic acid and polypeptides for a novel ecdysone receptor and a novel ultraspiracle receptor. The nucleic acid and polypeptides are useful in novel gene expression systems inducible with ecdysone or derivative receptor agonists.
摘要翻译： 本发明的领域是可诱导的基因表达系统，其中表达由新的蜕皮激素或超尖锐体受体或其衍生物控制。 该领域特别涉及用于新型蜕皮激素受体和新型超急性受体的核酸和多肽的分离和表征。 核酸和多肽可用于可用蜕皮激素或衍生物受体激动剂诱导的新基因表达系统。
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公开(公告)号：US07238859B2
公开(公告)日：2007-07-03
申请号：US10292356
申请日：2002-11-12
CPC分类号： C12N15/8287 , C07K14/43577 , C07K14/721 , C07K2319/00
摘要： The field of the invention is inducible gene expression systems wherein expression is controlled by a novel ecdysone or ultraspiracle receptor or its derivatives. The field particularly relates to the isolation and characterization of nucleic acid and polypeptides for a novel ecdysone receptor and a novel ultraspiracle receptor. The nucleic acid and polypeptides are useful in novel gene expression systems inducible with ecdysone or derivative receptor agonists.
摘要翻译： 本发明的领域是可诱导的基因表达系统，其中表达由新的蜕皮激素或超尖锐体受体或其衍生物控制。 该领域特别涉及用于新型蜕皮激素受体和新型超急性受体的核酸和多肽的分离和表征。 核酸和多肽可用于可用蜕皮激素或衍生物受体激动剂诱导的新基因表达系统。
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公开(公告)号：US06504082B1
公开(公告)日：2003-01-07
申请号：US09393839
申请日：1999-09-10
IPC分类号： A01N100
CPC分类号： C12N15/8287 , C07K14/43577 , C07K14/721 , C07K2319/00
摘要： The field of the invention is inducible gene expression systems wherein expression is controlled by a novel ecdysone receptor or its derivatives. The field particularly relates to the isolation and characterization of nucleic acid and polypeptides for a novel ecdysone receptor. The nucleic acid and polypeptides are useful in novel gene expression systems inducible with ecdysone or derivative receptor agonists.
摘要翻译： 本发明的领域是可诱导的基因表达系统，其中表达由新的蜕皮激素受体或其衍生物控制。 该领域特别涉及用于新型蜕皮激素受体的核酸和多肽的分离和表征。 核酸和多肽可用于可用蜕皮激素或衍生物受体激动剂诱导的新基因表达系统。
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公开(公告)号：US06037523A
公开(公告)日：2000-03-14
申请号：US880499
申请日：1997-06-23
IPC分类号： A01H5/00 , C07K14/34 , C12N5/10 , C12N9/10 , C12N9/22 , C12N9/24 , C12N15/09 , C12N15/29 , C12N15/82 , C12N15/00 , A01H1/06 , A01H4/00 , C12N5/00
CPC分类号： C12Y302/01031 , C07K14/34 , C12N15/8231 , C12N15/8289 , C12N9/1037 , C12N9/22 , C12N9/2402 , C12N9/93 , C12Y302/01039 , C12Y602/01003
摘要： The present invention relates to an isolated nucleic acid sequence encoding the Ms45 male tissue-preferred regulatory region. In one aspect this invention relates the use of this male tissue-preferred regulatory region in mediating fertility. An example of such use is the production of hybrid seed such as in a male sterility system. The Ms45 male tissue-preferred regulatory region can be operably linked with exogenous genes, such as those encoding cytotoxins, complementary nucleotidic units and inhibitory molecules. This invention also relates to plant cells, plant tissue and differentiated plants which contain the regulatory region in this invention.
摘要翻译： 本发明涉及编码Ms45雄性组织优选调节区的分离的核酸序列。 一方面，本发明涉及这种雄性组织优选的调节区在介导生育力中的用途。 这种用途的一个例子是杂交种子的生产，例如在雄性不育系统中。 可将Ms45雄性组织优选的调节区域与外源基因（例如编码细胞毒素，互补核苷酸单元和抑制性分子的那些）可操作地连接。 本发明还涉及在本发明中含有调节区的植物细胞，植物组织和分化植物。
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6.发明授权Male tissue-preferred regulatory sequences of Ms45 gene and method of using same 有权Ms45基因的男性组织优选调节序列及其使用方法公开(公告)号：US07154024B2
公开(公告)日：2006-12-26
申请号：US10713381
申请日：2003-11-14
CPC分类号： C12Y302/01031 , C07K14/34 , C07K14/415 , C12N9/1037 , C12N9/22 , C12N9/2434 , C12N15/8231 , C12N15/8289 , C12Y602/01003
摘要： The present invention relates to isolated male tissue-preferred regulatory sequences from Ms45 gene. The invention also relates to the use of Ms45 gene male tissue-preferred regulatory sequences in mediating male fertility in a plant, and in producing hybrid seed. The Ms45 male tissue-preferred regulatory region can be operably linked to exogenous genes encoding cytotoxins or inhibitory molecules. The invention also relates to plant cells, plant tissue and plants that contain the MS45 gene regulatory sequences.
摘要翻译： 本发明涉及来自Ms45基因的分离的男性组织优选的调节序列。 本发明还涉及Ms45基因雄性组织优选的调节序列在植物中介导雄性生育力和产生杂交种子中的用途。 可将Ms45雄性组织优选的调节区域可操作地连接到编码细胞毒素或抑制分子的外源基因。 本发明还涉及含有MS45基因调控序列的植物细胞，植物组织和植物。
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公开(公告)号：US08334429B2
公开(公告)日：2012-12-18
申请号：US12650106
申请日：2009-12-30
CPC分类号： C12N15/8205 , C12N9/1007
摘要： Auxotrophic Agrobacterium and methods employing auxotrophic Agrobacterium are provided. Auxotrophic Agrobacterium may be used in a variety of methods including biologically containing Agrobacterium comprising a transgene and transforming a plant cell without using an Agrobacterium counter-selective agent. Transforming maize immature embryos using an Agrobacterium auxotrophic for thymidine results in comparable transformation efficiency as transformation achieved using prototrophic Agrobacterium. Methods for producing an Agrobacterium thymidine auxotroph and using the auxotroph in transformation methods are disclosed. Transformed tissues and plants produced using methods of the present invention are also provided.
摘要翻译： 营养缺陷型农杆菌和使用营养缺陷型农杆菌的方法。 营养不良农杆菌可以以多种方法使用，包括生物地含有转基因的农杆菌，并且不使用农杆菌反选择剂来转化植物细胞。 使用农杆菌营养缺陷型用于胸苷转化玉米未成熟胚，导致与使用原养性农杆菌的转化相当的转化效率。 公开了用于生产农杆菌营养缺陷型农杆菌和在转化方法中使用营养缺陷型的方法。 还提供了使用本发明的方法生产的转化的组织和植物。
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8.发明授权公开(公告)号：US08618358B2
公开(公告)日：2013-12-31
申请号：US12947454
申请日：2010-11-16
申请人： Paul C. C. Feng , Agustin E. Fonseca , Carl W. Garnaat , Oscar Heredia , Jintai Huang , Rebecca A. Kelly , Youlin Qi , Martin A. Stoecker
发明人： Paul C. C. Feng , Agustin E. Fonseca , Carl W. Garnaat , Oscar Heredia , Jintai Huang , Rebecca A. Kelly , Youlin Qi , Martin A. Stoecker
CPC分类号： A01H5/10 , A01H1/02 , C12N15/8275 , C12N15/8289 , C12Q1/6895 , Y02E50/10 , Y02E50/30 , Y10T436/143333
摘要： The invention provides transgenic maize event MON 87427 and plants, plant cells, seeds, plant parts, and commodity products derived from event MON 87427. The invention also provides nucleotides specific for transgenic maize event MON 87427 and plants, plant cells, seeds, plant parts, and commodity products comprising nucleotides specific for transgenic maize event MON 87427. The invention also provides methods related to transgenic maize event MON 87427 and to the Roundup® Hybridization System (RHS). The invention also provides a Relative Development Scale useful for monitoring and determining reproductive development in maize that reconciles developmental differences across various maize varieties. This is useful for determining the optimal timing of a treatment regimen in which tassel development stage is an important factor, including various methods in making hybrid seed.
摘要翻译： 本发明提供转基因玉米事件MON 87427和源自事件MON 87427的植物，植物细胞，种子，植物部分和商品。本发明还提供转基因玉米事件MON 87427的特异性核苷酸和植物，植物细胞，种子，植物部分 以及包含对转基因玉米事件MON 87427具有特异性的核苷酸的商品。本发明还提供了与转基因玉米事件MON 87427和综合杂交系统（RHS）相关的方法。 本发明还提供了一个有助于监测和确定玉米生殖发育的相对发展规模，以调节各种玉米品种间的发育差异。 这可用于确定流苏开发阶段是重要因素的治疗方案的最佳时机，其中包括制作杂交种子的各种方法。
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9.发明申请公开(公告)号：US20110126310A1
公开(公告)日：2011-05-26
申请号：US12947454
申请日：2010-11-16
申请人： Paul C.C. Feng , Agustin E. Fonseca , Carl W. Garnaat , Oscar Heredia , Jintai Huang , Rebecca A. Kelly , Martin A. Stoecker
发明人： Paul C.C. Feng , Agustin E. Fonseca , Carl W. Garnaat , Oscar Heredia , Jintai Huang , Rebecca A. Kelly , Martin A. Stoecker
IPC分类号： A01H5/00 , C07H21/04 , A01H5/10 , C12N5/04 , C11B1/00 , A01N57/20 , G01N33/53 , A01H1/04 , C12Q1/68 , A01P13/00 , A01P21/00 , C12Q1/02 , C10L5/44 , A01G1/00 , A01C7/00 , A01D46/00 , A23K1/00 , A23L1/10 , A23L1/164
CPC分类号： A01H5/10 , A01H1/02 , C12N15/8275 , C12N15/8289 , C12Q1/6895 , Y02E50/10 , Y02E50/30 , Y10T436/143333
摘要： The invention provides transgenic maize event MON 87427 and plants, plant cells, seeds, plant parts, and commodity products derived from event MON 87427. The invention also provides nucleotides specific for transgenic maize event MON 87427 and plants, plant cells, seeds, plant parts, and commodity products comprising nucleotides specific for transgenic maize event MON 87427. The invention also provides methods related to transgenic maize event MON 87427 and to the Roundup® Hybridization System (RHS). The invention also provides a Relative Development Scale useful for monitoring and determining reproductive development in maize that reconciles developmental differences across various maize varieties. This is useful for determining the optimal timing of a treatment regimen in which tassel development stage is an important factor, including various methods in making hybrid seed.
摘要翻译： 本发明提供转基因玉米事件MON 87427和源自事件MON 87427的植物，植物细胞，种子，植物部分和商品。本发明还提供转基因玉米事件MON 87427的特异性核苷酸和植物，植物细胞，种子，植物部分 以及包含对转基因玉米事件MON 87427具有特异性的核苷酸的商品。本发明还提供了与转基因玉米事件MON 87427和综合杂交系统（RHS）相关的方法。 本发明还提供了一个有助于监测和确定玉米生殖发育的相对发展规模，以调节各种玉米品种间的发育差异。 这可用于确定流苏开发阶段是重要因素的治疗方案的最佳时机，其中包括制作杂交种子的各种方法。
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公开(公告)号：US5824864A
公开(公告)日：1998-10-20
申请号：US449986
申请日：1995-05-25
IPC分类号： C07K14/415 , C12N15/29 , C12N15/82 , C12N15/00 , A01H5/00
CPC分类号： C12N15/8286 , A01N65/00 , A01N65/44 , C07K14/415
摘要： Nucleotide sequences encoding a maize 5C9 polypeptide, the 5C9 polypeptide and structural and functional equivalents, and methods for controlling insect infestation in plants are provided.
摘要翻译： 提供编码玉米5C9多肽的核苷酸序列，5C9多肽及其结构和功能等同物，以及控制植物昆虫侵袭的方法。
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