公开(公告)号：US09150856B2

公开(公告)日：2015-10-06

申请号：US14097659

申请日：2013-12-05

Applicant: Novozymes, Inc.

Inventor： Debbie Yaver ,  Peter Nham

IPC: C12P21/00 ,  C12N15/113 ,  C12N15/80 ,  C12P1/02

CPC classification number: C12N15/80 ,  C12N9/0044 ,  C12N9/20 ,  C12N9/58 ,  C12N9/93 ,  C12N15/113 ,  C12P1/02 ,  C12P21/00 ,  C12Y107/01003 ,  C12Y301/01003 ,  C12Y304/21004 ,  C12Y603/04002

Abstract: The present invention relates to methods for producing a biological substance, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the biological substance, wherein the fungal host cell comprises a first nucleic acid sequence encoding the biological substance operably linked to a second nucleic acid sequence comprising a promoter variant selected from the group consisting of SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, and SEQ ID NO: 12; and a subsequence thereof; and hybrid and tandem promoters thereof; and (b) isolating the biological substance from the cultivation medium. The present invention also relates to the isolated promoter variants and to constructs, vectors, and fungal host cells comprising the promoter variants operably linked to nucleic acid sequences encoding biological substances.

Abstract translation: 本发明涉及生物体的制造方法，其特征在于，包括：（a）在有助于生物生物的培养基中培养真菌宿主细胞，其中所述真菌宿主细胞可操作地包含编码生物物质的第一核酸序列 连接到第二核酸序列，其包含选自SEQ ID NO：2，SEQ ID NO：3，SEQ ID NO：4，SEQ ID NO：5，SEQ ID NO：6，SEQ ID NO： ：7，SEQ ID NO：8，SEQ ID NO：9，SEQ ID NO：10，SEQ ID NO：11和SEQ ID NO：12; 及其后果; 及其杂交和串联启动子; 和（b）从培养基中分离生物物质。 本发明还涉及分离的启动子变体和构建体，载体和真菌宿主细胞，其包含可操作地连接于编码生物物质的核酸序列的启动子变体。

公开(公告)号：US08999685B2

公开(公告)日：2015-04-07

申请号：US14269591

申请日：2014-05-05

Applicant: Novozymes, Inc.

Inventor： Stephen Brown ,  Sheryl Luttringer ,  Debbie Yaver ,  Alan Berry

IPC: C12P7/46 ,  C12N1/00 ,  C07K14/38 ,  C12N15/80

CPC classification number: C12P7/46 ,  C07K14/38 ,  C12N15/80

Abstract: The present invention relates to methods of producing a C4 dicarboxylic acid, comprising: (a) cultivating a filamentous fungal host cell comprising a polynucleotide selected from the group consisting of a heterologous first polynucleotide encoding a C4 dicarboxylic acid transporter, a heterologous second polynucleotide encoding a malate dehydrogenase, and a heterologous third polynucleotide encoding a pyruvate carboxylase; wherein the filamentous fungal host cell is capable of secreting increased levels of the C4 dicarboxylic acid compared to the filamentous fungal host cell without the heterologous polynucleotide when cultivated under the same conditions; and (b) recovering the C4 dicarboxylic acid. The present invention also relates to methods for increasing C4 dicarboxylic acid production, filamentous fungal host cells and malate dehydrogenase variants.

公开(公告)号：US20140234925A1

公开(公告)日：2014-08-21

申请号：US14269591

申请日：2014-05-05

Applicant: Novozymes, Inc.

Inventor： Stephen Brown ,  Sheryl Luttringer ,  Debbie Yaver ,  Alan Berry

IPC: C12P7/46

CPC classification number: C12P7/46 ,  C07K14/38 ,  C12N15/80

Abstract: The present invention relates to methods of producing a C4 dicarboxylic acid, comprising: (a) cultivating a filamentous fungal host cell comprising a polynucleotide selected from the group consisting of a heterologous first polynucleotide encoding a C4 dicarboxylic acid transporter, a heterologous second polynucleotide encoding a malate dehydrogenase, and a heterologous third polynucleotide encoding a pyruvate carboxylase; wherein the filamentous fungal host cell is capable of secreting increased levels of the C4 dicarboxylic acid compared to the filamentous fungal host cell without the heterologous polynucleotide when cultivated under the same conditions; and (b) recovering the C4 dicarboxylic acid. The present invention also relates to methods for increasing C4 dicarboxylic acid production, filamentous fungal host cells and malate dehydrogenase variants.

Abstract translation: 本发明涉及生产C4二羧酸的方法，其包括：（a）培养丝状真菌宿主细胞，所述丝状真菌宿主细胞包含选自以下的多核苷酸：编码C4二羧酸转运蛋白的异源第一多核苷酸，编码 苹果酸脱氢酶和编码丙酮酸羧化酶的异源第三多核苷酸; 其中当在相同条件下培养时，丝状真菌宿主细胞能够分泌与不含异源多核苷酸的丝状真菌宿主细胞相比增加的C4二羧酸水平; 和（b）回收C4二羧酸。 本发明还涉及增加C4二羧酸生产，丝状真菌宿主细胞和苹果酸脱氢酶变体的方法。

公开(公告)号：US20140227391A1

公开(公告)日：2014-08-14

申请号：US14104756

申请日：2013-12-12

Applicant: NOVOZYMES, INC.

Inventor： Suzanne Otani ,  Haiyan Ge ,  Paul Harris ,  Debbie Yaver ,  Alexander Blinkovsky

IPC: C12N9/26 ,  C12C5/00 ,  C12C7/04

CPC classification number: C12N9/2408 ,  C12C5/004 ,  C12C7/04 ,  C12C7/20 ,  C12C11/00 ,  C12C12/02 ,  C12N9/2411 ,  C12N15/8245 ,  C12Y302/0102

Abstract: The present invention relates to isolated polypeptides having alpha-glucosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.

Abstract translation: 本发明涉及具有α-葡糖苷酶活性的分离多肽和编码多肽的分离的多核苷酸。 本发明还涉及包含多核苷酸的核酸构建体，载体和宿主细胞以及生产和使用多肽的方法。