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    Method of using α-amylase from Aspergillus clavatus for saccharification
    4.
    发明授权
    Method of using α-amylase from Aspergillus clavatus for saccharification 有权
    使用曲霉菌α-淀粉酶进行糖化的方法

    公开(公告)号：US09365871B2

    公开(公告)日：2016-06-14

    申请号：US14561085

    申请日：2014-12-04

    申请人： DANISCO US INC.

    发明人： Jing Ge ,  Ling Hua ,  Martijn Silvan Scheffers ,  Zhongmei Tang ,  Marco Van Brussel-Zwijnen ,  Casper Vroemen ,  Bo Zhang ,  Kathleen A. Clarkson ,  Jacquelyn A. Huitink ,  Paula Johanna Maria Teunissen

    IPC分类号： C12P19/14 ,  C12C7/04 ,  C12P7/14

    CPC分类号： C12P7/14 ,  C12C7/04 ,  C12P19/14 ,  C12P2201/00 ,  Y02E50/17

    摘要： A fungal α-amylase is provided from Aspergillus clavatus (AcAmy1). AcAmy1 has an optimal pH of 4.5 and is operable at 30-75° C., allowing the enzyme to be used in combination with a glucoamylase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the α-amylase or glucoamylase. AcAmy1 also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DP1+DP2) compared to the products of saccharification catalyzed by an α-amylase from Aspergillus kawachii. This facilitates the utilization of the oligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.

    摘要翻译： 真菌α-淀粉酶由曲霉菌（AcAmy1）提供。 AcAmy1具有4.5的最佳pH，并且在30-75℃下可操作，允许酶在糖化反应中与葡糖淀粉酶组合使用。 这消除了作为间歇法运行糖化反应的必要性，其中必须重新调节pH和温度以最佳地使用α-淀粉酶或葡糖淀粉酶。 与由川曲霉α-淀粉酶催化的糖化产物相比，AcAmy1还催化淀粉底物对显着富含DP2和（DP1 + DP2）的寡糖组合物的糖化。 这有助于例如在同时糖化和发酵过程中由发酵生物体利用寡糖组合物。

    METHOD OF USING ALPHA-AMYLASE FROM TALAROMYCES EMERSONII FOR SACCHARIFICATION
    6.
    发明申请
    METHOD OF USING ALPHA-AMYLASE FROM TALAROMYCES EMERSONII FOR SACCHARIFICATION 审中-公开
    使用来自TALAROMESCES EMERSONII的ALPHA-AMYLASE进行鉴定的方法

    公开(公告)号：US20150240223A1

    公开(公告)日：2015-08-27

    申请号：US14433941

    申请日：2013-09-17

    申请人： DANISCO US INC.

    发明人： Ling Hua ,  Zhongmei Tang ,  Casper Willem Vroemen ,  Bo Zhang ,  Zhenghong Zhang

    IPC分类号： C12N9/30 ,  C12P7/14 ,  A21D8/04 ,  C11D3/386 ,  C12C11/07 ,  C12P19/02 ,  C12P19/14

    CPC分类号： C12N9/242 ,  A21D8/042 ,  A23L29/35 ,  C11D3/386 ,  C12C5/004 ,  C12C7/04 ,  C12C7/14 ,  C12C11/003 ,  C12C11/07 ,  C12P7/14 ,  C12P19/02 ,  C12P19/14 ,  C12Y302/01001 ,  D06L1/12 ,  D06L1/14 ,  D06M16/003 ,  Y02E50/16 ,  Y02E50/17

    摘要： A fungal alpha amylase is provided from Talaromyces emersonii (TeAmy1), along with variants of the same. TeAmy1 has an optimal pH of 3.5 and is operable over a temperature range of at least 30 75° C., allowing the enzyme to be used in combination with a glucoamylase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the alpha amylase or glucoamylase. TeAmy1 also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DP1+DP2) compared to the products of saccharification catalyzed by an alpha amylase from Aspergillus kawachii. This facilitates the utilization of the oligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.

    摘要翻译： 真菌α淀粉酶从艾美（Talaromyces emersonii）（TeAmy1）提供，以及相同的变体。 TeAmy1具有3.5的最佳pH，并且在至少3075℃的温度范围内是可操作的，允许酶在糖化反应中与葡糖淀粉酶组合使用。 这消除了作为间歇方法运行糖化反应的必要性，其中必须重新调节pH和温度以最佳地使用α淀粉酶或葡糖淀粉酶。 与由来自曲霉（Aspergillus kawachii）的α淀粉酶催化的糖化产物相比，TeAmy1还催化淀粉底物对显着富集DP2和（DP1 + DP2）的寡糖组合物的糖化。 这有助于例如在同时糖化和发酵过程中由发酵生物体利用寡糖组合物。

    USE OF SILICATE PARTICLES IN BEVERAGE PRODUCTION
    9.
    发明申请
    USE OF SILICATE PARTICLES IN BEVERAGE PRODUCTION 审中-公开
    硅酸钠颗粒在饮料生产中的应用

    公开(公告)号：US20140120211A1

    公开(公告)日：2014-05-01

    申请号：US14066320

    申请日：2013-10-29

    申请人： Erbsloh Geisenheim AG

    发明人： Jürgen Fröhlich ,  Volker Müller ,  Anne Besier

    IPC分类号： C12C11/11

    CPC分类号： A23L2/38 ,  A23L2/52 ,  A23L2/80 ,  C12C7/04 ,  C12C11/00 ,  C12H1/0408

    摘要： The use of a material in the production of a beverage, wherein the material particles are added in the course of the production process to a starting material or an intermediate product of the beverage and are then separated off again prior to finishing of the beverage, wherein the particles of the material are characterised in that they at least partially comprise a silicate, wherein the silicate particles with respect to the total weight of the particles have a proportion of at least one metal ion in the range of between 1.5 and 30% by weight, wherein the at least one metal ion is selected from a metal ion of the elements La, Ce, Cu, Ag, Zn, Sn, Ca, Mg, Fe and Mn.

    摘要翻译： 在生产饮料中使用材料，其中将材料颗粒在生产过程中加入到饮料的原料或中间产物中，然后在饮料完成之前再分离，其中 材料的颗粒的特征在于它们至少部分地包含硅酸盐，其中硅酸盐颗粒相对于颗粒的总重量具有至少一种金属离子的比例在1.5至30重量％的范围内 其中，所述至少一种金属离子选自元素La，Ce，Cu，Ag，Zn，Sn，Ca，Mg，Fe和Mn的金属离子。

    BEER BREWING SYSTEM AND METHOD
    10.
    发明申请
    BEER BREWING SYSTEM AND METHOD 审中-公开
    啤酒酿造系统和方法

    公开(公告)号：US20140017354A1

    公开(公告)日：2014-01-16

    申请号：US13935684

    申请日：2013-07-05

    申请人： James Joseph ,  Brandy Callanan

    发明人： James Joseph ,  Brandy Callanan

    IPC分类号： C12C7/04

    CPC分类号： C12C7/04 ,  C12C13/10

    摘要： The present subject matter relates to systems and methods for automated, whole grain brewing. In one configuration, such a system can include a base, a boil kettle positioned on the base, a first heating element in communication with the boil kettle and configured to selectively heat fluid contained in the boil kettle, and a mash tun positioned on the base, the mash tun configured to receive one or more solid or fluid materials therein. A pumping system positioned at least partially within the base can be connected to the boil kettle and the mash tun, the pumping system being operable to selectively pass fluid into, out of, and among the boil kettle and the mash tun. In addition, a control system can be positioned at least partially within the base and configured to selectively control the first heating element and the pumping system.

    摘要翻译： 本主题涉及用于自动化，全谷物酿造的系统和方法。 在一种配置中，这种系统可以包括基座，位于基座上的煮沸壶，与煮沸壶连通并构造成选择性地加热包含在煮水壶中的流体的第一加热元件和位于基座上的麦芽壶 所述麦芽壶配置成在其中接收一种或多种固体或流体材料。 至少部分地位于基座内的泵送系统可以连接到沸水壶和泥泞管道，泵送系统可操作以选择性地将流体传送到沸水壶和泥泞管道内部，以及其中。 此外，控制系统可以至少部分地定位在基座内并且被配置为选择性地控制第一加热元件和泵送系统。