公开(公告)号：US07102058B2

公开(公告)日：2006-09-05

申请号：US10629950

申请日：2003-07-20

申请人： Rebecca E. Cahoon ,  Scott V. Tingey

发明人： Rebecca E. Cahoon ,  Scott V. Tingey

IPC分类号： A01H1/00 ,  C07H21/04 ,  C07K14/415 ,  C12N5/14 ,  C12N9/00

CPC分类号： C12N9/1205 ,  C12N9/16 ,  C12N15/8243

摘要： This invention relates to an isolated nucleic acid fragment encoding a phytic acid biosynthetic enzyme. The invention also relates to the construction of a chimeric gene encoding all or a portion of the phytic acid biosynthetic enzyme, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the phytic acid biosynthetic enzyme in a transformed host cell.

摘要翻译： 本发明涉及编码植酸生物合成酶的分离的核酸片段。 本发明还涉及在正义或反义方向上编码全部或部分植酸生物合成酶的嵌合基因的构建，其中嵌合基因的表达导致转化的植酸生物合成酶水平的变化 宿主细胞。

公开(公告)号：US07087813B2

公开(公告)日：2006-08-08

申请号：US10059909

申请日：2002-01-29

申请人： Anthony J. Kinney ,  Theodore M. Klein ,  Jian-Ming Lee ,  Richard W. Pearlstein ,  J. Antoni Rafalski ,  Jennie Bih-Jien Shen ,  Catherine J. Thorpe ,  Scott V. Tingey

发明人： Anthony J. Kinney ,  Theodore M. Klein ,  Jian-Ming Lee ,  Richard W. Pearlstein ,  J. Antoni Rafalski ,  Jennie Bih-Jien Shen ,  Catherine J. Thorpe ,  Scott V. Tingey

IPC分类号： A01H1/00 ,  C07H21/04 ,  C07K14/415 ,  C12N5/14 ,  C12N9/00

CPC分类号： C12N9/0069 ,  C12N15/8279

摘要： This invention relates to isolated nucleic acid fragments encoding lipoxygenases. The invention also relates to the construction of recombinant DNA constructs encoding all or a portion of the lipoxygenase, in sense or antisense orientation, wherein expression of the recombinant DNA construct results in production of altered levels of the lipoxygenase in a transformed host cell.

公开(公告)号：US5126239A

公开(公告)日：1992-06-30

申请号：US494258

申请日：1990-03-14

申请人： Kenneth J. Livak ,  Jan A. Rafalski ,  Scott V. Tingey ,  John G. Williams

发明人： Kenneth J. Livak ,  Jan A. Rafalski ,  Scott V. Tingey ,  John G. Williams

IPC分类号： C12N15/09 ,  C12Q1/68

CPC分类号： C12Q1/6858 ,  Y10T436/143333

摘要： A process is provided for detecting polymorphisms on the basis of nucleotide differences in random segments of the nucleic acid by performing a primer extension reaction on the nucleic acids and comparing the extension reaction products. The random nucleic acid segment can be amplified by first dissociating the extension product from the template and contacting the dissociated extension product with a primer under conditions such that an amplification extension product is synthesized using the dissociated extension product as a template. Differences in the extension product are useful as markers in constructing genetic maps and as markers to distinguish or identify individuals.

摘要翻译： 提供了通过对核酸进行引物延伸反应并比较延伸反应产物来检测基于核酸随机区段的核苷酸差异的多态性的方法。 可以通过首先将延伸产物从模板中解离并使解离的延伸产物与引物接触并在使用解离的延伸产物作为模板合成扩增产物的条件下扩增随机核酸片段。 扩展产物的差异可用作构建遗传图谱的标记和用于区分或识别个体的标记。