Method for culturing cells in order to produce substances
    41.
    发明申请
    Method for culturing cells in order to produce substances 审中-公开
    培养细胞以产生物质的方法

    公开(公告)号:US20060127975A1

    公开(公告)日:2006-06-15

    申请号:US10535581

    申请日:2003-11-07

    摘要: The invention concerns a method for culturing cells in order to produce substances. According to the invention a cell line producing substances is cultured while feeding a nutrient medium in such a manner that glucose limitation occurs in the culture solution. The degree of glucose limitation DGL=qGlc/qGlcmax (qGlc=observed current specific glucose consumption rate; qGlcmax=maximum known specific glucose consumption rate for these cells). DGL is between the limits 0 and 1, where 0 means complete limitation and 1 means no limitations or complete glucose excess. According to the invention DGL is larger or equal to the DGL which only leads to the maintenance of the cell and ≦0.5.

    摘要翻译: 本发明涉及一种培养细胞以产生物质的方法。 根据本发明,在以培养液中发生葡萄糖限制的方式饲养营养培养基的同时培养生产细胞的物质。 葡萄糖限制程度DGL = qGlc / qGlc最大值(qGlc =观察到的当前特定葡萄糖消耗速率; qGlc最大值=这些细胞的最大已知特定葡萄糖消耗速率)。 DGL在极限0和1之间,其中0表示完全限制,1表示无限制或完全葡萄糖过量。 根据本发明,DGL大于或等于DGL,其仅导致细胞的维持和<= 0.5。

    Reflector lamp
    44.
    发明授权
    Reflector lamp 失效
    反光灯

    公开(公告)号:US06060820A

    公开(公告)日:2000-05-09

    申请号:US973702

    申请日:1997-12-08

    CPC分类号: H01K1/66 H01J5/60 H01K1/40

    摘要: A reflector lamp may be made with a reflector and base holding a hermeticy sealed bulb. The bulb encloses a filling and a light source, the light source being a high voltage or medium voltage light source in which an operating voltage of at least 80 volts is present. The reflector consists of a basic body with a reflector contour and a neck part attached to a rear portion of the basic body, and a base which is joined to the neck part and which has at least two metallic contact pins with cylindrical seals on an end farthest from the bulb. A current feeder system makes an electrical lead available for use by the light source. The base is formed completely from the same material as the neck part and as an integral part directly on the neck part of the reflector.

    摘要翻译: PCT No.PCT / DE97 / 00750 Sec。 371 1997年12月8日第 102(e)日期1997年12月8日PCT 1997年4月12日提交PCT公布。 出版物WO97 / 40511 日期1997年10月30日反射灯可以制成具有保持密封的灯泡的反射器和基座。 灯泡包围填充物和光源,光源是高压或中压光源,其中存在至少80伏的工作电压。 反射器由具有反射器轮廓的基体和连接到基体的后部的颈部部分组成,底座连接到颈部,并且具有至少两个金属接触销,端部具有圆柱形密封 距离灯泡最远 当前的馈线系统使电源可供光源使用。 基部完全由与颈部相同的材料形成,并且作为整体部分直接形成在反射器的颈部上。