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公开(公告)号：US20080182254A1
公开(公告)日：2008-07-31
申请号：US11926062
申请日：2007-10-28
IPC分类号： C12Q1/68
CPC分类号： C12N9/1252 , C12N9/22 , C12Q1/6823 , C12Q1/683 , C12Q1/701 , C12Q1/703 , C12Q1/706 , Y10S435/81 , C12Q2561/109
摘要： The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. The present invention further relates to methods and devices for the separation of nucleic acid molecules based on charge. The present invention also provides methods for the detection of non-target cleavage products via the formation of a complete and activated protein binding region. The invention further provides sensitive and specific methods for the detection of nucleic acid from various viruses in a sample.
摘要翻译： 本发明涉及核酸序列的检测和表征以及核酸序列的变化的方法。 本发明还涉及在靶序列上形成核酸切割结构并以位点特异性方式切割核酸切割结构的方法。 使用各种酶的结构特异性核酸酶活性来切割靶依赖性切割结构，从而指示特异性核酸序列的存在或其特定变体。 本发明还涉及基于电荷分离核酸分子的方法和装置。 本发明还提供了通过形成完整和活化的蛋白质结合区域来检测非靶切割产物的方法。 本发明还提供用于检测样品中各种病毒的核酸的敏感和具体方法。
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公开(公告)号：US07354708B2
公开(公告)日：2008-04-08
申请号：US10033297
申请日：2001-11-02
CPC分类号： C12N9/22 , C12N9/1252 , C12Q1/6816 , C12Q1/6823 , C12Q1/6827 , C12Q1/683 , C12Q1/70 , C12Q1/701 , C12Q2525/143 , C12Q2521/301 , C12Q2525/155 , C12Q2525/131 , C12Q2561/109 , C12Q2537/149 , C12Q2525/186
摘要： The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. The present invention further relates to methods and devices for the separation of nucleic acid molecules based on charge. The present invention also provides methods for the detection of non-target cleavage products via the formation of a complete and activated protein binding region. The invention further provides sensitive and specific methods for the detection of human cytomegalovirus nucleic acid in a sample.
摘要翻译： 本发明涉及核酸序列的检测和表征以及核酸序列的变化的方法。 本发明还涉及在靶序列上形成核酸切割结构并以位点特异性方式切割核酸切割结构的方法。 使用各种酶的结构特异性核酸酶活性来切割靶依赖性切割结构，从而指示特异性核酸序列的存在或其特定变体。 本发明还涉及基于电荷分离核酸分子的方法和装置。 本发明还提供了通过形成完整和活化的蛋白质结合区域来检测非靶切割产物的方法。 本发明还提供用于检测样品中巨细胞病毒核酸的敏感和具体方法。
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公开(公告)号：US06872816B1
公开(公告)日：2005-03-29
申请号：US09381212
申请日：1998-03-24
申请人： Jeff G. Hall , Victor I. Lyamichev , Andrea L. Mast , Mary Ann D. Brow , Robert W. Kwiatkowski , Stephanie H. Vavra
发明人： Jeff G. Hall , Victor I. Lyamichev , Andrea L. Mast , Mary Ann D. Brow , Robert W. Kwiatkowski , Stephanie H. Vavra
CPC分类号： C12N9/1252 , C12N9/22 , C12Q1/6823 , C12Q1/683 , C12Q1/701 , C12Q1/703 , C12Q1/706 , Y10S435/81 , C12Q2561/109
摘要： The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. The present invention further relates to methods and devices for the separation of nucleic acid molecules based on charge. The present invention also provides methods for the detection of non-target cleavage products via the formation of a complete and activated protein binding region. The invention further provides sensitive and specific methods for the detection of nucleic acid from various viruses in a sample.
摘要翻译： 本发明涉及核酸序列的检测和表征以及核酸序列的变化的方法。 本发明还涉及在靶序列上形成核酸切割结构并以位点特异性方式切割核酸切割结构的方法。 使用各种酶的结构特异性核酸酶活性来切割靶依赖性切割结构，从而指示特异性核酸序列的存在或其特定变体。 本发明还涉及基于电荷分离核酸分子的方法和装置。 本发明还提供了通过形成完整和活化的蛋白质结合区域来检测非靶切割产物的方法。 本发明还提供用于检测样品中各种病毒的核酸的敏感和具体方法。
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44.发明授权公开(公告)号：US06458535B1
公开(公告)日：2002-10-01
申请号：US09350597
申请日：1999-07-09
IPC分类号： C12Q168
CPC分类号： C12N9/22 , C12N9/1252 , C12Q1/6816 , C12Q1/6823 , C12Q1/6827 , C12Q1/683 , C12Q1/70 , C12Q1/701 , C12Q2525/143 , C12Q2521/301 , C12Q2525/155 , C12Q2525/131 , C12Q2561/109 , C12Q2537/149 , C12Q2525/186
摘要： The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. The present invention further relates to methods and devices for the separation of nucleic acid molecules based on charge. The present invention also provides methods for the detection of non-target cleavage products via the formation of a complete and activated protein binding region. The invention further provides sensitive and specific methods for the detection of human cytomegalovirus nucleic acid in a sample.
摘要翻译： 本发明涉及核酸序列的检测和表征以及核酸序列的变化的方法。 本发明还涉及在靶序列上形成核酸切割结构并以位点特异性方式切割核酸切割结构的方法。 使用各种酶的结构特异性核酸酶活性来切割靶依赖性切割结构，从而指示特异性核酸序列的存在或其特定变体。 本发明还涉及基于电荷分离核酸分子的方法和装置。 本发明还提供了通过形成完整和活化的蛋白质结合区域来检测非靶切割产物的方法。 本发明还提供用于检测样品中巨细胞病毒核酸的敏感和具体方法。
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公开(公告)号：US6090543A
公开(公告)日：2000-07-18
申请号：US759038
申请日：1996-12-02
IPC分类号： G01N33/50 , C12N1/21 , C12N9/12 , C12N9/16 , C12N9/22 , C12N15/09 , C12Q1/68 , G01N33/566 , C12P19/34
CPC分类号： C12N9/22 , C12Q1/6823 , C12Q1/6827 , C12Q1/683 , Y10S435/81 , Y10S435/822
摘要： The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
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