公开(公告)号：US20130316376A1

公开(公告)日：2013-11-28

申请号：US13880628

申请日：2011-10-25

Applicant: My Hedhammar ,  Jan Johansson ,  Anna Rising ,  Per Åke Nygren

Inventor： My Hedhammar ,  Jan Johansson ,  Anna Rising ,  Per Åke Nygren

IPC: C07K14/435

CPC classification number: C07K14/43518 ,  B01D15/20 ,  B01D15/3804 ,  B01D15/3809 ,  B01D15/3823 ,  B01J20/281 ,  C07D495/04 ,  C07K14/31 ,  C07K14/76 ,  C07K16/065 ,  C07K2319/01 ,  C07K2319/21 ,  C07K2319/22 ,  C07K2319/70 ,  C07K2319/705 ,  C12N5/0068 ,  C12N2533/50 ,  Y10S210/905

Abstract: A protein structure capable of selective interaction with an organic target is provided. The protein structure is a polymer comprising as a repeating structural unit a recombinant fusion protein that is capable of selective interaction with the organic target. The fusion protein is comprising the moieties B, REP and CT, and optionally NT. B is a non-spidroin moiety of more than 30 amino acid residues, which provides the capacity of selective interaction with the organic target. REP is a moiety of from 70 to 300 amino acid residues and is derived from the repetitive fragment of a spider silk protein. CT is a moiety of from 70 to 120 amino acid residues and is derived from the C-terminal fragment of a spider silk protein. NT is an optional moiety of from 100 to 160 amino acid residues and is derived from the N-terminal fragment of a spider silk protein. The fusion protein and protein structure thereof is useful as an affinity medium and a cell scaffold material.

公开(公告)号：US20130065278A1

公开(公告)日：2013-03-14

申请号：US13634519

申请日：2010-10-27

Applicant: Jan Johansson ,  Anna Rising ,  My Hedhammar ,  Kerstin Nordling

Inventor： Jan Johansson ,  Anna Rising ,  My Hedhammar ,  Kerstin Nordling

IPC: C07K19/00 ,  C12N15/62 ,  C12P21/02 ,  C12N9/96

CPC classification number: C07K14/43518 ,  C12N15/62

Abstract: A method of producing a desired non-spidroin protein or polypeptide is comprising the steps of expressing in a suitable host a fusion protein, obtaining a mixture containing the fusion protein, and optionally isolating the fusion protein. The fusion protein is comprising at least one solubility-enhancing moiety which is derived from the N-terminal (NT) fragment of a spider silk protein. It is further comprising at least one moiety which is a desired non-spidroin protein or polypeptide. Each solubility-enhancing moiety is linked directly or indirectly to the desired protein or polypeptide moiety.

Abstract translation: 产生所需非宿主蛋白或多肽的方法包括以下步骤：在合适的宿主中表达融合蛋白，获得含有融合蛋白的混合物，以及任选地分离融合蛋白。 融合蛋白质包含衍生自蜘蛛丝蛋白的N末端（NT）片段的至少一种溶解度增强部分。 其还包含至少一个作为期望的非宿主蛋白或多肽的部分。 每个溶解度增强部分直接或间接连接到所需的蛋白质或多肽部分。