公开(公告)号：US20060063180A1

公开(公告)日：2006-03-23

申请号：US11202063

申请日：2005-08-12

Applicant: Yoshihiro Yamashita ,  Toshinari Sakurai

Inventor： Yoshihiro Yamashita ,  Toshinari Sakurai

IPC: C12Q1/68 ,  C07H21/04 ,  C12N1/08

CPC classification number: C12N15/1006

Abstract: It is an object of the present invention to release and isolate nucleic acid from a biological sample readily and in a short time. The present invention relates to a technique for isolating nucleic acid by which a biological sample including cells is caused to pass through a solid substance carrier to isolate the cells, a mixture of the cells and a cell lysis reagent is caused to pass through the solid substance carrier to disrupt the cells and release nucleic acid therein, and a mixture of the released nucleic acid and a nucleic acid binding reagent is caused to pass through the solid substance carrier to bind the nucleic acid to the solid substance carrier. Examples of an instrument comprising such solid substance carrier include an instrument comprising a syringe having a solid substance carrier fixed therein. The instrument is capable of moving a solution from one space to another space separated by the solid substance carrier, using pressurization and depressurization via the syringe, thereby causing the solution to pass through the solid substance carrier. Preferably, the solid substance carrier is a solid substance having a multitude of channels therein or a mesh-like solid substance composed of fibrous substances, by which cells are disrupted from a biological sample including the cells, nucleic acid is released, and the nucleic acid can be bound.

Abstract translation: 本发明的目的是容易且短时间地从生物样品中释放和分离核酸。 本发明涉及一种用于分离核酸的技术，通过该技术使包含细胞的生物样品通过固体物质载体以分离细胞，使细胞和细胞裂解试剂的混合物通过固体物质 载体以破坏细胞并释放其中的核酸，并且使释放的核酸和核酸结合试剂的混合物通过固体物质载体以将核酸结合至固体物质载体。 包括这种固体物质载体的仪器的实例包括一种仪器，其包括固定有固体物质载体的注射器。 该仪器能够通过经由注射器的加压和减压将溶液从一个空间移动到由固体物质载体分离的另一个空间，从而使溶液通过固体物质载体。 优选地，固体物质载体是其中具有多个通道的固体物质或由纤维物质组成的网状固体物质，细胞从包括细胞的生物样品中被破坏，核酸被释放，并且核酸 可以束缚

公开(公告)号：US06705830B2

公开(公告)日：2004-03-16

申请号：US10131643

申请日：2002-04-23

Applicant: Yoshihiro Yamashita ,  Yoshiyuki Sakaguchi ,  Yasushi Maejima

Inventor： Yoshihiro Yamashita ,  Yoshiyuki Sakaguchi ,  Yasushi Maejima

IPC: F04D1904

CPC classification number: F04D19/04 ,  F04D27/0292 ,  F04D29/601

Abstract: A vacuum pump has an end portion and a flange extending from a periphery of the end portion. The flange portion has first and second opposed main surfaces, and first and second bolt insertion holes extending through the first and second main surfaces. An auxiliary ring is connected to the first main surface of the flange portion of the pump case. The auxiliary ring has first and second opposed main surfaces, and a third bolt insertion hole extending through the first and second main surfaces of the auxiliary ring. An auxiliary ring attaching bolt extends through the third bolt insertion hole for connecting the auxiliary ring to a chamber housing disposed opposite the end portion of the pump case. A pump case supporting bolt extends through the second bolt insertion hole of the pump case flange portion and is connected to the auxiliary ring, and a pump case fastening bolt extends through the first bolt insertion hole of the pump case flange portion and is connected to the auxiliary ring.

Abstract translation: 真空泵具有从端部的周边延伸的端部和凸缘。 凸缘部具有第一和第二相对的主表面，以及延伸穿过第一和第二主表面的第一和第二螺栓插入孔。 辅助环连接到泵壳的凸缘部分的第一主表面。 辅助环具有第一和第二相对的主表面，以及延伸穿过辅助环的第一和第二主表面的第三螺栓插入孔。 辅助环安装螺栓延伸穿过第三螺栓插入孔，用于将辅助环连接到与泵壳的端部相对设置的腔室。 泵壳支撑螺栓延伸穿过泵壳凸缘部分的第二螺栓插入孔并连接到辅助环，泵壳紧固螺栓延伸穿过泵壳凸缘部分的第一螺栓插入孔，并连接到 辅助环。

公开(公告)号：US06435605B2

公开(公告)日：2002-08-20

申请号：US09874051

申请日：2001-06-06

Applicant: Ryo Tsuruo ,  Kenichi Kuriyama ,  Masaki Ohmori ,  Nobuo Ito ,  Yoshifumi Horiuchi ,  Masanobu Ohtsu ,  Hiroshi Okada ,  Yoshihiro Yamashita

Inventor： Ryo Tsuruo ,  Kenichi Kuriyama ,  Masaki Ohmori ,  Nobuo Ito ,  Yoshifumi Horiuchi ,  Masanobu Ohtsu ,  Hiroshi Okada ,  Yoshihiro Yamashita

IPC: B60J700

CPC classification number: B60J7/22 ,  B60J7/053

Abstract: A vehicle sunroof has a tilting lid provided at a front part of an opening formed in a roof of a vehicle. The tilting lid is adapted to be moved vertically at a rear end thereof with lifter mechanisms. A sliding lid is provided at a rear part of the opening supported by sliders adapted to be moved along guide rails disposed on both side edge portions of the opening and is constructed so as to be moved longitudinally through such movements of the sliders. The lifter mechanisms are provided in such a manner as to be oriented laterally at portions facing a rear end of the tilting lid on a laterally oriented cross member disposed so as to extend between front end portions of the left and right guide rails.

Abstract translation: 车辆天窗具有设置在形成于车辆顶部的开口的前部的倾斜盖。 倾斜盖适于在其后端处垂直移动，具有升降机构。 滑动盖设置在开口的后部，由适于沿着设置在开口的两个侧边缘部分上的导轨移动的滑块支撑，并且构造成通过滑块的这种移动而纵向移动。 升降机构以这样的方式设置，即在横向定位的横向构件上面向倾斜盖的后端的部分侧向定向，以便在左右导轨的前端部之间延伸。

公开(公告)号：US06312047B1

公开(公告)日：2001-11-06

申请号：US09330014

申请日：1999-06-11

Applicant: Ryo Tsuruo ,  Kenichi Kuriyama ,  Masaki Ohmori ,  Nobuo Ito ,  Yoshifumi Horiuchi ,  Masanobu Ohtsu ,  Hiroshi Okada ,  Yoshihiro Yamashita

Inventor： Ryo Tsuruo ,  Kenichi Kuriyama ,  Masaki Ohmori ,  Nobuo Ito ,  Yoshifumi Horiuchi ,  Masanobu Ohtsu ,  Hiroshi Okada ,  Yoshihiro Yamashita

IPC: B60J7053

CPC classification number: B60J7/22 ,  B60J7/053

Abstract: A vehicle sunroof has a tilting lid provided at a front part of an opening formed in a roof of a vehicle. The tilting lid is adapted to be moved vertically at a rear end thereof with lifter mechanisms. A sliding lid is provided at a rear part of the opening supported by sliders adapted to be moved along guide rails disposed on both side edge portions of the opening and is constructed so as to be moved longitudinally through such movements of the sliders. The lifter mechanisms are provided in such a manner as to be oriented laterally at portions facing a rear end of the tilting lid on a laterally oriented cross member disposed so as to extend between front end portions of the left and right guide rails.

Abstract translation: 车辆天窗具有设置在形成于车辆顶部的开口的前部的倾斜盖。 倾斜盖适于在其后端处垂直移动，具有升降机构。 滑动盖设置在开口的后部，由适于沿着设置在开口的两个侧边缘部分上的导轨移动的滑块支撑，并且构造成通过滑块的这种移动而纵向移动。 升降机构以这样的方式设置，即在横向定位的横向构件上面向倾斜盖的后端的部分侧向定向，以便在左右导轨的前端部之间延伸。