公开(公告)号：US20060147963A1

公开(公告)日：2006-07-06

申请号：US11313480

申请日：2005-12-20

申请人： Anthony Barone ,  Handong Li ,  Glenn McGall

发明人： Anthony Barone ,  Handong Li ,  Glenn McGall

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6816 ,  C12N15/1096 ,  C12Q1/6806 ,  C12Q1/6837 ,  C12Q2600/158 ,  C12Q2525/113 ,  C12Q2565/501 ,  C12Q2521/107 ,  C12Q2525/143 ,  C12Q2525/117

摘要： Methods are provided for detecting hybridization of a polynucleotide to a nucleic acid array by chemically modifying the polynucleotide to contain a detectable label. According to one aspect of the present invention, a method is provided for detecting the presence of a mRNA in a nucleic acid sample, the method having the steps of providing a mRNA sample and azido modified nucleotides, hybridizing a primer to the mRNA, reversed transcribing the mRNA to provide azido modified DNA, followed by reacting the azido groups with a detectable label, hybridizing the labeled RNA to a nucleic acid array and detecting the presence of the mRNA. Still other methods are provided for detecting the presence or absence of a polynucleotide of interest on a nucleic acid array, the method having the steps of providing a nucleic acid sample comprising a polynucleotide; providing an enzyme to amplify the polynucleotide using an azido nucleotide derivative; amplifying said polynucleotide to provide azido labeled amplified nucleic acids; reacting the azido groups on said nucleic acids with a detectable label to provide labeled nucleic acids; hybridizing said amplified nucleic acids to a nucleic acid array; and detecting the presence or absence of said polynucleotide. Still other methods are presented for detecting polynucleotides on a nucleic acid array using ligases and terminal transferases to end label polynucleotides.

摘要翻译： 提供了通过化学修饰多核苷酸以含有可检测标记物来检测多核苷酸与核酸阵列的杂交的方法。 根据本发明的一个方面，提供了一种用于检测核酸样品中mRNA的存在的方法，该方法具有以下步骤：提供mRNA样品和叠氮基修饰的核苷酸，将引物与mRNA杂交，逆转录 mRNA以提供叠氮基修饰的DNA，然后使叠氮基与可检测标记反应，将标记的RNA与核酸阵列杂交并检测mRNA的存在。 还提供了用于在核酸阵列上检测感兴趣的多核苷酸的存在或不存在的其它方法，所述方法具有提供包含多核苷酸的核酸样品的步骤; 提供使用叠氮基核苷酸衍生物扩增多核苷酸的酶; 扩增所述多核苷酸以提供叠氮基标记的扩增核酸; 使所述核酸上的叠氮基与可检测标记物反应以提供标记的核酸; 将所述扩增的核酸与核酸阵列杂交; 并检测所述多核苷酸的存在或不存在。 还提出了使用连接酶和末端转移酶终止标记多核苷酸检测核酸阵列上的多核苷酸的其它方法。

公开(公告)号：US07033520B2

公开(公告)日：2006-04-25

申请号：US10667928

申请日：2003-09-22

申请人： William K. Kappel ,  Venkatappa Viswanatha ,  Handong Li ,  Richard J. Mehigh ,  John G. Dapron

发明人： William K. Kappel ,  Venkatappa Viswanatha ,  Handong Li ,  Richard J. Mehigh ,  John G. Dapron

IPC分类号： C09K3/00

CPC分类号： G01N33/5306 ,  B01J45/00 ,  C07B2200/11 ,  C07C323/58 ,  G01N33/6803 ,  Y10T436/25125

摘要： A metal chelating composition having the formula: wherein Q is a carrier; S1 is a spacer; L is -A-T-CH(X)— or —C(═O)—; A is an ether, thioether, selenoether, or amide linkage; T is a bond or substituted or unsubstituted alkyl or alkenyl; X is —(CH2)kCH3, —(CH2)kCOOH, —(CH2)kSO3H, —(CH2)kPO3H2, —(CH2)kN(J)2, or —(CH2)kP(J)2, preferably —(CH2)kCOOH or —(CH2)kSO3H; k is an integer from 0 to 2; J is hydrocarbyl or substituted hydrocarbyl; Y is —COOH, —H, —SO3H, —PO3H2, —N(J)2, or —P(J)2, preferably, —COOH; Z is —COOH, —H, —SO3H, —PO3H2, —N(J)2, or —P(J)2, preferably, —COOH; and i is an integer from 0 to 4, preferably 1 or 2.

公开(公告)号：US06623655B1

公开(公告)日：2003-09-23

申请号：US09558001

申请日：2000-04-24

申请人： William K. Kappel ,  Venkatappa Viswanatha ,  Handong Li ,  Richard J. Mehigh ,  John G. Dapron

发明人： William K. Kappel ,  Venkatappa Viswanatha ,  Handong Li ,  Richard J. Mehigh ,  John G. Dapron

IPC分类号： C09K300

CPC分类号： G01N33/5306 ,  B01J45/00 ,  C07B2200/11 ,  C07C323/58 ,  G01N33/6803 ,  Y10T436/25125

摘要： A metal chelating composition having the formula: wherein Q is a carrier; S1 is a spacer; L is —A—T—CH(X)— or —C(═O)—; A is an ether, thioether, selenoether, or amide linkage; T is a bond or substituted or unsubstituted alkyl or alkenyl; X is —(CH2)kCH3, —(CH2)kCOOH, —(CH2)kSO3H, —(CH2)kPO3H2, —(CH2)kN(J)2, or —(CH2)kP (J)2, preferably —(CH2)kCOOH or —(CH2)kSO3H; k is an integer from 0 to 2; J is hydrocarbyl or substituted hydrocarbyl; Y is —COOH, —H, —SO3H, —PO3H2, —N(J)2, or —P(J)2, preferably, —COOH; Z is —COOH, —H, —SO3H, —PO3H2, —N(J)2, or —P(J)2, preferably, —COOH; and i is an integer from 0 to 4, preferably 1 or 2.

摘要翻译： 一种具有下式的金属螯合组合物：其中Q是载体; S 1是间隔基; L是-AT-CH（X） - 或-C（= O） - ; A是醚，硫醚，硒醚或 酰胺键; T是键或取代或未取代的烷基或链烯基; X是 - （CH2）kCH3， - （CH2）kCOOH， - （CH2）kSO3H， - （CH2）kPO3H2， - （CH2）kN（J） ，或 - （CH 2）k P（J）2，优选 - （CH 2）k COOH或 - （CH 2）k SO 3 H; k是0-2的整数; J是烃基或取代的烃基; Y是-COOH， SO 3 H，-PO 3 H 2，-N（J）2或-P（J）2，优选-COOH; Z是-COOH，-H，-SO 3 H，-PO 3 H 2，-N（J）2或-P J）2，优选-COOH; andi是0至4，优选1或2的整数。