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公开(公告)号：US20240018605A1
公开(公告)日：2024-01-18
申请号：US18352722
申请日：2023-07-14
IPC分类号： C12Q1/689 , C12Q1/6848 , C12Q1/04
CPC分类号： C12Q1/689 , C12Q1/6848 , C12Q1/04 , C12Q2521/101 , C12Q2525/113 , C12Q2525/101 , G01N2333/91245
摘要： Methods of detecting the absence or presence of a micro-organism in a sample comprising: contacting the sample with a nucleic acid molecule which acts as a substrate for nucleic acid modifying activity of the micro-organism in the sample, incubating the thus contacted sample under conditions suitable for nucleic acid modifying activity; and specifically determining the absence or presence of a modified nucleic acid molecule resulting from the action of the nucleic acid modifying activity on the substrate nucleic acid molecule to indicate the absence or presence of the micro-organism.
Corresponding kits are also provided.-
公开(公告)号：US11827932B2
公开(公告)日：2023-11-28
申请号：US17153309
申请日：2021-01-20
申请人： Illumina, Inc.
发明人： Robert C. Kain , Xiaohai Liu , Wenyi Feng , Bernard Hirschbein , Helmy A. Eltoukhy , Xiaolin Wu , Geoffrey Paul Smith , Jonathan Mark Boutell , Thomas Joseph , Randall Smith , Min-Jui Richard Shen , Carolyn Tregidgo , Kay Klausing
IPC分类号： C12Q1/68 , C12Q1/6874 , C12Q1/6869
CPC分类号： C12Q1/6874 , C12Q1/6869 , C12Q1/6874 , C12Q2525/113 , C12Q2535/122 , C12Q2563/107
摘要： The present disclosure provides methods and systems for detecting multiple different nucleotides in a sample. In particular, the disclosure provides for detection of multiple different nucleotides in a sample utilizing fewer detection moieties than the number of nucleotides being detected and/or fewer imaging events than the number of nucleotides being detected.
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公开(公告)号：US11821032B2
公开(公告)日：2023-11-21
申请号：US16999329
申请日：2020-08-21
发明人： Luisa Andruzzi , Jimmy Adediran , Timothy Pelletier , Austin Ricker , Angela DeLucia , Ben McNally , Dona Hevroni , Mong Sano Marma , John Andrew Sheridan
IPC分类号： C12P19/34 , C12Q1/6869 , C12Q1/6876 , C12Q1/6806 , C07D275/03
CPC分类号： C12Q1/6869 , C12Q1/6806 , C12Q1/6876 , C07D275/03 , C12Q2525/113 , C12Q2525/186 , C12Q2600/16 , C12Q1/6869 , C12Q2525/113 , C12Q2525/117 , C12Q2525/186
摘要： The present invention provides methods, compositions, mixtures and kits utilizing 5-Chloro-2-methyl-4-isothiazolin-3-one in sequencing reactions, and in particular, sequencing reactions where deoxynucleoside triphosphates comprising a 3′-O position capped by a disulfide-based 3′-terminator group are used. In one embodiment, the deoxynucleoside triphosphates comprise a 3′-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. In addition, thiol-containing compounds and scavengers of thio-containing compounds are described. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis.
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公开(公告)号：US20190024175A1
公开(公告)日：2019-01-24
申请号：US16032707
申请日：2018-07-11
发明人： Yanbing Zu , Jackie Y. Ying
IPC分类号： C12Q1/6883 , G01N21/78 , C12Q1/6827 , C12Q1/6881
CPC分类号： C12Q1/6883 , C12Q1/6827 , C12Q1/6881 , C12Q2600/106 , C12Q2600/156 , C12Q2600/158 , C12Q2600/16 , G01N21/78 , C12Q2525/113 , C12Q2527/107 , C12Q2563/149
摘要： A method and kit for detecting a genetic variant associated with a disease or disorder, including incompatibility with a pharmaceutical. The method and kit using a first nano-particle coupled to at least one morpholino nucleic acid probe comprising a target complimentary region base sequence that is a perfect match to a genetic variant sequence.
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公开(公告)号：US20180195131A1
公开(公告)日：2018-07-12
申请号：US15746395
申请日：2016-07-21
IPC分类号： C12Q1/6886 , C12Q1/6827
CPC分类号： C12Q1/6886 , C12Q1/6827 , C12Q2600/156 , C12Q2600/158 , C12Q2525/113 , C12Q2535/122 , C12Q2565/519
摘要： Provided herein is a method for enriching a sample for polynucleotides comprising a breakpoint of a fusion gene, comprising: a) contacting a probe set comprising a plurality of polynucleotide probes, each probe configured to specifically hybridize to a fusion gene, wherein the set comprises one or more high affinity polynucleotide probes (e.g., a polynucleotide comprising one or more locked nucleic acid nucleotides), with a mixture of polynucleotides under hybridization conditions to produce probe-captured polynucleotides; and b) isolating the probe-captured polynucleotides from the mixture, to produce a sample enriched with polynucleotides comprising breakpoint fragments of the fusion gene.
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公开(公告)号：US20180148772A1
公开(公告)日：2018-05-31
申请号：US15825575
申请日：2017-11-29
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6825 , C12Q1/6837 , C12Q2600/178 , C12Q2525/113 , C12Q2563/116 , C12Q2563/157 , C12Q2565/501 , C12Q2565/607
摘要： The present invention relates to a method for establishing a quantitative landscape of a group of target nucleic acid molecules. The method comprises conducting a plurality of hybridization reactions for quantifying each target nucleic acid molecule of the group of target nucleic acid molecules to generate a plurality of quantitative signals; generating ratios between two quantitative signals; and consolidating the ratios for constructing the quantitative landscape of the group of target nucleic acid molecules. The method according to the invention is able to profile numerous target nucleic acid molecules to provide a big data standardization means for a variety of applications with high sensitivity and wide dynamic range.
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7.发明申请公开(公告)号：US20180100191A1
公开(公告)日：2018-04-12
申请号：US15809558
申请日：2017-11-10
发明人： Susan H. Hardin , Xiaolian Gao , James Briggs , Richard Willson , Shiao-Chun Tu
CPC分类号： C12Q1/6869 , C07H19/06 , C07H19/10 , C07H19/20 , C12N9/1241 , C12N9/1252 , C12Q1/6806 , C12Q1/6853 , C12Q2525/101 , C12Q2565/301 , C12Q2533/101 , C12Q2525/113
摘要： Nucleotide triphosphate probes containing a molecular and/or atomic tag on a γ and/or β phosphate group and/or a base moiety having a detectable property are disclosed, and kits and method for using the tagged nucleotides in sequencing reactions and various assay. Also, phosphate and polyphosphate molecular fidelity altering agents are disclosed.
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8.发明授权公开(公告)号：US09845500B2
公开(公告)日：2017-12-19
申请号：US14977167
申请日：2015-12-21
发明人： Susan H. Hardin , Xiaolian Gao , James Briggs , Richard Willson , Shiao-Chun Tu
CPC分类号： C12Q1/6869 , C07H19/06 , C07H19/10 , C07H19/20 , C12N9/1241 , C12N9/1252 , C12Q1/6806 , C12Q1/6853 , C12Q2525/101 , C12Q2565/301 , C12Q2533/101 , C12Q2525/113
摘要： Nucleotide triphosphate probes containing a molecular and/or atomic tag on a γ and/or β phosphate group and/or a base moiety having a detectable property are disclosed, and kits and method for using the tagged nucleotides in sequencing reactions and various assay. Also, phosphate and polyphosphate molecular fidelity altering agents are disclosed.
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公开(公告)号：US09822401B2
公开(公告)日：2017-11-21
申请号：US14688764
申请日：2015-04-16
申请人： GenapSys, Inc.
发明人： Florian Oberstrass
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6848 , C12Q1/6853 , C12Q2531/119 , C12Q2565/519 , C12Q2521/507 , C12Q2525/107 , C12Q2525/113 , C12Q2527/101 , C12Q2531/107 , C12Q2535/122 , C12Q2537/1373 , C12Q2537/162 , C12Q2565/537
摘要： The disclosure provides methods and systems for nucleic acid amplification including isothermal nucleic acid amplification.
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公开(公告)号：US20170247756A1
公开(公告)日：2017-08-31
申请号：US15515758
申请日：2015-09-28
发明人： Edgar Schreiber , Kamini Varma , Mark Andersen
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , C12Q1/6806 , C12Q1/6853 , C12Q1/6858 , C12Q1/6869 , C12Q2537/143 , C12Q2537/149 , C12Q2537/159 , C12Q2549/119 , C12Q2535/122 , C12Q2565/125 , C12Q2521/325 , C12Q2525/113 , C12Q2525/125 , C12Q2535/101
摘要： Methods, compositions and kits are described for resequencing, confirming or verifying Next Generation Sequencing (NGS) results with Sanger Sequencing. These methods are particularly useful for samples having very limited quantities such as formalin-fixed, paraffin-embedded (FFPE), Laser Capture Microdissection (LCM), fine needle biopsies or aspirates.
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