公开(公告)号：US20200299708A1

公开(公告)日：2020-09-24

申请号：US16763385

申请日：2018-10-31

Applicant: Dow AgroSciences LLC

Inventor： Sandeep Kumar ,  Andrew Asberry ,  Pierluigi Barone ,  Rodrigo Sarria ,  Stephen Foulk ,  Nagesh Sardesai

IPC: C12N15/82

Abstract: This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a promoter from a Zea mays PR1 gene. Some embodiments relate to a promoter or a 5′ UTR from a Zea mays PR1 gene that functions in plants to promote transcription of operably linked nucleotide sequences. Other embodiments relate to a 3′ UTR or a terminator from a Zea mays PR1 gene that functions in plants to promote transcription of operably linked nucleotide sequences.

公开(公告)号：US20200248197A1

公开(公告)日：2020-08-06

申请号：US16856536

申请日：2020-04-23

Applicant: Dow AgroSciences LLC

Inventor： Sandeep Kumar ,  Marcelo Ariel German ,  Pahao Wang ,  Todd P. Glancy ,  Shreedharan Sriram ,  Carla N. Yerkes ,  Andrew J. Bowling ,  Heather Pence ,  Andrew E. Robinson

IPC: C12N15/82

Abstract: This disclosure concerns the use of endogenous plant RNAi machinery to preferentially or specifically reduce transgene expression. In some embodiments, the disclosure concerns specific reduction of transgene expression in seed tissues of a dicot plant.

公开(公告)号：US10450577B2

公开(公告)日：2019-10-22

申请号：US15835620

申请日：2017-12-08

Applicant: Dow AgroSciences LLC

Inventor： Sandeep Kumar ,  Daren Hemingway ,  Carla Ausmus ,  Andrew F. Worden ,  Andrew Asberry

IPC: C12N15/82 ,  C07K14/825

Abstract: This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a promoter from a Panicum virgatum metallothionein-like gene (mtl). Some embodiments relate to a promoter from a Panicum virgatum metallothionein-like gene (mtl) that functions in plants to promote transcription of operably linked nucleotide sequences.

公开(公告)号：US20180094271A1

公开(公告)日：2018-04-05

申请号：US15698714

申请日：2017-09-08

Applicant: Dow AgroSciences LLC

Inventor： Sandeep Kumar ,  Pierluigi Barone ,  Daren Hemingway ,  Emily Etchison ,  Andrew Asberry ,  Heather Pence ,  Andrew J. Bowling

IPC: C12N15/82

Abstract: This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a promoter from a Panicum virgatum (Pavir.J00490) egg cell gene. Some embodiments relate to a promoter from a Panicum virgatum (Pavir.J00490) egg cell gene that functions in plants to promote transcription of operably linked nucleotide sequences. Other embodiments relate to a 3′ UTR from a Panicum virgatum (Pavir.J00490) egg cell gene that functions in plants to promote transcription of operably linked nucleotide sequences.

公开(公告)号：US20180087063A1

公开(公告)日：2018-03-29

申请号：US15835595

申请日：2017-12-08

Applicant: Dow AgroSciences LLC

Inventor： Sandeep Kumar ,  Daren Hemingway ,  Carla Ausmus ,  Andrew F. Worden ,  Andrew Asberry

IPC: C12N15/82

Abstract: This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a promoter from a Brachypodium distachyon metallothionein-like gene (mtl). Some embodiments relate to a promoter from a Brachypodium distachyon metallothionein-like gene (mtl) that functions in plants to promote transcription of operably linked nucleotide sequences.

公开(公告)号：US20170362600A1

公开(公告)日：2017-12-21

申请号：US15612024

申请日：2017-06-02

Applicant: Dow AgroSciences LLC

Inventor： Sandeep Kumar ,  Andrew F. Worden ,  Stephen Novak ,  Ryan M. Lee

IPC: C12N15/82 ,  A01H1/02

Abstract: The current disclosure relates to methods and compositions for improving plant varieties through plant breeding and plant genetics. For instance, the disclosure concerns increasing the recombination frequency of a heterozygous trait genetically linked to a second trait within plants. Further, the disclosure concerns breaking the genetic linkage between a first allele and a second allele.

公开(公告)号：US09752154B2

公开(公告)日：2017-09-05

申请号：US14416799

申请日：2013-07-23

Applicant: DOW AGROSCIENCES LLC ,  Sandeep Kumar ,  Steven L. Evans ,  Manju Gupta

Inventor： Sandeep Kumar ,  Steven L. Evans ,  Manju Gupta

IPC: C12N15/68 ,  C12N15/82

CPC classification number: C12N15/8209 ,  C12N15/68 ,  C12N15/82

Abstract: This invention is related to methods and systems for vector assembly for transgenic plants. A uniform modular process is used to reduce cycle time and the methods and systems provided herein can increase cloning throughput using multiple-well plates, for example 96-well plates. In some embodiments, the methods and systems provided herein eliminate or reduce the need for sequencing confirmation because no PCR is involved in the vector assembly process.

公开(公告)号：US09688996B2

公开(公告)日：2017-06-27

申请号：US14416811

申请日：2013-08-08

Applicant: DOW AGROSCIENCES LLC ,  Sandeep Kumar ,  Manju Gupta ,  Diaa Alabed

Inventor： Sandeep Kumar ,  Manju Gupta ,  Diaa Alabed

IPC: C12N15/82

CPC classification number: C12N15/8216 ,  C12N15/8218

Abstract: Provided are methods, vectors and gene constructs for enhancing expression of a recombinant nucleic acid sequence in transgenic plants and plant tissues. According to the present invention, nucleic acid sequences are obtained and/or derived from the 3′ untranslated regions of genes encoding ubiquitin proteins and engineered to flank respective portions of a selected coding region of a vector. The vector construct may be introduced into plants and/or plant tissues through conventional or gene targeting procedures, resulting in enhanced expression of the selected coding region. In some embodiments, the selected coding region is a chimeric gene or gene fragment expressing one or more proteins known to impart a level of insecticidal activity to a transgenic plant and/or plant tissue.

公开(公告)号：US20160304894A1

公开(公告)日：2016-10-20

申请号：US15097345

申请日：2016-04-13

Applicant: Dow AgroSciences LLC

Inventor： Sandeep Kumar ,  Daren Hemingway ,  Carla Ausmus ,  Andrew F. Worden ,  Andrew Asberry

IPC: C12N15/82

CPC classification number: C12N15/8227

Abstract: This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a promoter from a Brachypodium distachyon metallothionein-like gene (mtl). Some embodiments relate to a promoter from a Brachypodium distachyon metallothionein-like gene (mtl) that functions in plants to promote transcription of operably linked nucleotide sequences.

Abstract translation: 本公开涉及用于促进植物或植物细胞中的核苷酸序列的转录的组合物和方法，其使用来自鹦鹉螺金属硫蛋白金属硫蛋白样基因（mt1）的启动子。 一些实施方案涉及在植物中起作用以促进可操作地连接的核苷酸序列的转录的来自鹦鹉螺金属硫蛋白样金丝桃样基因（mt1）的启动子。

公开(公告)号：US20130198898A1

公开(公告)日：2013-08-01

申请号：US13674712

申请日：2012-11-12

Applicant: Dow AgroSciences LLC

Inventor： Sandeep Kumar ,  Diaa Alabed ,  Sara Bennett ,  Manju Gupta ,  Susan Jayne ,  Terry R. Wright

IPC: C12N15/82

CPC classification number: C12N15/8216 ,  C12N15/8286 ,  Y02A40/162

Abstract: Provided are constructs and methods for expressing multiple genes in plant cells and/or plant tissues. The constructs provided comprise at least one bi-directional promoter linked to multiple gene expression cassettes, wherein the bi-directional promoter comprises a functional promoter nucleotide sequence from Sugar Cane Bacilliform Virus promoter. In some embodiments, the constructs and methods provided employs a bi-directional promoter based on a minimal core promoter element from a Zea mays Ubiquitin-1 gene, or a functional equivalent thereof, and nucleotide sequence elements from a Sugar Cane Bacilliform Virus promoter. In some embodiments, the constructs and methods provided allow expression of genes between three and twenty.

Abstract translation: 提供了用于在植物细胞和/或植物组织中表达多个基因的构建体和方法。 所提供的构建体包括至少一个与多个基因表达盒连接的双向启动子，其中双向启动子包含来自甘蔗芽孢杆菌病毒启动子的功能启动子核苷酸序列。 在一些实施方案中，所提供的构建体和方法采用基于来自玉米泛素-1基因或其功能等同物的最小核心启动子元件和来自甘蔗芽孢杆菌病毒启动子的核苷酸序列元件的双向启动子。 在一些实施方案中，提供的构建体和方法允许在3至20个之间表达基因。