公开(公告)号：US10781455B2

公开(公告)日：2020-09-22

申请号：US15612024

申请日：2017-06-02

Applicant: Dow AgroSciences LLC

Inventor： Sandeep Kumar ,  Andrew F. Worden ,  Stephen Novak ,  Ryan M. Lee

IPC: C12N15/82

Abstract: The current disclosure relates to methods and compositions for improving plant varieties through plant breeding and plant genetics. For instance, the disclosure concerns increasing the recombination frequency of a heterozygous trait genetically linked to a second trait within plants. Further, the disclosure concerns breaking the genetic linkage between a first allele and a second allele.

公开(公告)号：US20170204425A1

公开(公告)日：2017-07-20

申请号：US15471545

申请日：2017-03-28

Applicant: Dow AgroSciences LLC ,  Sangamo Therapeutics, Inc.

Inventor： Russell DeKelver ,  Manju Gupta ,  Jeffrey C. Miller ,  Stephen Novak ,  Joseph F. Petolino

IPC: C12N15/82

CPC classification number: C12N15/8218 ,  C07K14/415 ,  C12N9/22 ,  C12N15/8216 ,  C12N15/8247 ,  C12Y203/01041 ,  C12Y301/00

Abstract: The present disclosure relates to engineered zinc finger proteins that target genes in plants involved in fatty acid biosynthesis. Methods of using such zinc finger proteins in modulating gene expression, gene inactivation, and targeted gene modification are also provided.

公开(公告)号：US08889390B2

公开(公告)日：2014-11-18

申请号：US13766380

申请日：2013-02-13

Applicant: Dow AgroSciences LLC ,  Sangamo BioSciences, Inc.

Inventor： Manju Gupta ,  Asha M. Palta ,  Stephen Novak ,  Fyodor Urnov ,  Sunita Gopalan

IPC: C12N9/00 ,  C12N15/82 ,  C12N9/10

CPC classification number: C12N15/8213 ,  C07K2319/00 ,  C07K2319/81 ,  C12N9/1092 ,  C12N15/8243 ,  C12N15/8274

Abstract: The present disclosure relates to engineered zinc finger proteins that target 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS) genes in plants and methods of using such zinc finger proteins in modulating gene expression, gene inactivation, and targeted gene modification. In particular, the disclosure pertains to zinc finger nucleases for targeted cleavage and alteration of EPSPS genes.

Abstract translation: 本公开涉及靶向植物中5-烯醇丙酮酸莽草酸-3-磷酸合酶（EPSPS）基因的工程化锌指蛋白，以及使用这种锌指蛋白调节基因表达，基因失活和靶向基因修饰的方法。 特别地，本公开涉及用于靶向切割和改变EPSPS基因的锌指核酸酶。

公开(公告)号：US20140157458A1

公开(公告)日：2014-06-05

申请号：US14089260

申请日：2013-11-25

Applicant: Sangamo BioSciences, Inc. ,  Dow AgroSciences LLC

Inventor： Russell DeKelver ,  Manju Gupta ,  Jeffrey C. Miller ,  Stephen Novak ,  Joseph F. Petolino

IPC: C12N15/82 ,  C12N9/22 ,  C07K14/415

CPC classification number: C12N15/8218 ,  C07K14/415 ,  C12N9/22 ,  C12N15/8216 ,  C12N15/8247 ,  C12Y203/01041 ,  C12Y301/00

Abstract: The present disclosure relates to engineered zinc finger proteins that target genes in plants involved in fatty acid biosynthesis. Methods of using such zinc finger proteins in modulating gene expression, gene inactivation, and targeted gene modification are also provided.

Abstract translation: 本公开涉及靶向参与脂肪酸生物合成的植物中的基因的工程化锌指蛋白。 还提供了使用这种锌指蛋白调节基因表达，基因失活和靶向基因修饰的方法。

公开(公告)号：US09631201B2

公开(公告)日：2017-04-25

申请号：US14089260

申请日：2013-11-25

Applicant: Dow AgroSciences LLC ,  Sangamo BioSciences, Inc.

Inventor： Russell DeKelver ,  Manju Gupta ,  Jeffrey C. Miller ,  Stephen Novak ,  Joseph F. Petolino

IPC: C12N15/82 ,  C07K14/415 ,  C12N9/22

CPC classification number: C12N15/8218 ,  C07K14/415 ,  C12N9/22 ,  C12N15/8216 ,  C12N15/8247 ,  C12Y203/01041 ,  C12Y301/00

Abstract: The present disclosure relates to engineered zinc finger proteins that target genes in plants involved in fatty acid biosynthesis. Methods of using such zinc finger proteins in modulating gene expression, gene inactivation, and targeted gene modification are also provided.

公开(公告)号：US09150877B2

公开(公告)日：2015-10-06

申请号：US13653602

申请日：2012-10-17

Applicant: Dow AgroSciences LLC

Inventor： Manju Gupta ,  Sean Michael Russell ,  Liu Yin Shen ,  Sivarama Reddy Chennareddy ,  Jyoti Rout ,  Stephen Novak

IPC: C12N15/113 ,  C12N15/82 ,  C12N9/10

CPC classification number: C12N15/8278 ,  C07K2319/00 ,  C07K2319/81 ,  C12N9/1092 ,  C12N15/113 ,  C12N15/8243 ,  C12N15/8274 ,  C12N15/8275 ,  C12N2830/205 ,  C12Y205/01019

Abstract: Provided are constructs and methods for expressing multiple genes in plant cells and/or plant tissues using a disclosed bidirectional promoter from Brassica napus or Brassica bidirectional constitutive promoter (BBCP). The constructs provided comprise at least one such bi-directional promoter linked to multiple gene expression cassettes, wherein each of the gene expression cassettes comprises at least one transgene. In some embodiments, the constructs and methods provided allow expression of genes between two and twenty.

Abstract translation: 提供了使用公开的来自甘蓝型油菜或芸苔属双侧组成型启动子（BBCP）的双向启动子在植物细胞和/或植物组织中表达多个基因的构建体和方法。 提供的构建体包含至少一个与多个基因表达盒连接的双向启动子，其中每个基因表达盒包含至少一个转基因。 在一些实施方案中，提供的构建体和方法允许在二至二十个之间表达基因。

公开(公告)号：US20150064790A1

公开(公告)日：2015-03-05

申请号：US14538609

申请日：2014-11-11

Applicant: Dow AgroSciences LLC ,  Sangamo BioSciences, Inc.

Inventor： Manju Gupta ,  Asha M. Palta ,  Stephen Novak ,  Fyodor Urnov ,  Sunita Gopalan

IPC: C12N15/82

CPC classification number: C12N15/8213 ,  C07K2319/00 ,  C07K2319/81 ,  C12N9/1092 ,  C12N15/8243 ,  C12N15/8274

Abstract: The present disclosure relates to engineered zinc finger proteins that target 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS) genes in plants and methods of using such zinc finger proteins in modulating gene expression, gene inactivation, and targeted gene modification. In particular, the disclosure pertains to zinc finger nucleases for targeted cleavage and alteration of EPSPS genes.

Abstract translation: 本公开涉及靶向植物中5-烯醇丙酮酸莽草酸-3-磷酸合酶（EPSPS）基因的工程化锌指蛋白，以及使用这种锌指蛋白调节基因表达，基因失活和靶向基因修饰的方法。 特别地，本公开涉及用于靶向切割和改变EPSPS基因的锌指核酸酶。

公开(公告)号：US10344289B2

公开(公告)日：2019-07-09

申请号：US14538609

申请日：2014-11-11

Applicant: Dow AgroSciences LLC ,  Sangamo BioSciences, Inc.

Inventor： Manju Gupta ,  Asha M. Palta ,  Stephen Novak ,  Fyodor Urnov ,  Sunita Gopalan

IPC: C12N15/66 ,  C12N15/62 ,  C12N15/82 ,  C12N9/10

Abstract: The present disclosure relates to engineered zinc finger proteins that target 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS) genes in plants and methods of using such zinc finger proteins in modulating gene expression, gene inactivation, and targeted gene modification. In particular, the disclosure pertains to zinc finger nucleases for targeted cleavage and alteration of EPSPS genes.

公开(公告)号：US10000806B2

公开(公告)日：2018-06-19

申请号：US14720511

申请日：2015-05-22

Applicant: Dow AgroSciences LLC

Inventor： Tina Marie Kaiser ,  Stephen Novak

IPC: C12Q1/68

CPC classification number: C12Q1/6876 ,  C12Q1/6813 ,  C12Q1/686 ,  C12Q2600/13 ,  C12Q2600/16 ,  C12Q2525/161 ,  C12Q2531/113 ,  C12Q2537/16 ,  C12Q2545/114 ,  C12Q2561/109

Abstract: Disclosed herein are methods for determining if a contaminating integration of a nucleotide sequence is present in a set of nucleic acids. Further disclosed herein are methods for determining the copy number/zygosity of a nucleic acid sequence of interest. The methods disclosed herein may be performed using quantitative PCR.

公开(公告)号：US20140090113A1

公开(公告)日：2014-03-27

申请号：US14020575

申请日：2013-09-06

Applicant: Dow AgroSciences LLC

Inventor： Noel Cogan ,  John Forster ,  Matthew Hayden ,  Tim Sawbridge ,  German Spangenberg ,  Steven R. Webb ,  Manju Gupta ,  W. Mike Ainley ,  Matthew J. Henry ,  John Mason ,  Sandeep Kumar ,  Stephen Novak

IPC: C12N15/82

Abstract: An Engineered Transgene Integration Platform (ETIP) is described that can be inserted randomly or at targeted locations in plant genomes to facilitate rapid selection and detection of a GOI that is perfectly targeted (both the 5′ and 3′ ends) at the ETIP genomic location. One element in the subject disclosure is the introduction of specific double stranded breaks within the ETIP. In some embodiments, an ETIP is described using zinc finger nuclease binding sites, but may utilize other targeting technologies such as meganucleases, CRISPRs, TALs, or leucine zippers. Also described are compositions of, and methods for producing, transgenic plants wherein the donor or payload DNA expresses one or more products of an exogenous nucleic acid sequence (e.g. protein or RNA) that has been stably-integrated into an ETIP in a plant cell. In embodiments, the ETIP facilitates testing of gene candidates and plant expression vectors from ideation through Development phases.

Abstract translation: 描述了可以随机或在植物基因组中的目标位置插入工程化转基因整合平台（ETIP），以便于在ETIP基因组位置上完全靶向（5'和3'末端）的GOI的快速选择和检测 。 主题披露中的一个要素是在ETIP内引入特定的双链断裂。 在一些实施方案中，使用锌指核酸酶结合位点描述ETIP，但可以利用其它靶向技术，例如大范围核酸酶，CRISPR，TAL或亮氨酸拉链。 还描述了转基因植物的组合物和生产方法，其中供体或有效载体DNA表达已经稳定整合到植物细胞中的ETIP中的外源核酸序列（例如蛋白质或RNA）的一种或多种产物。 在实施方案中，ETIP有助于从构想到开发阶段测试基因候选物和植物表达载体。