公开(公告)号：US10047367B2

公开(公告)日：2018-08-14

申请号：US14514833

申请日：2014-10-15

申请人： Dow AgroSciences LLC

发明人： Manju Gupta ,  Navin Elango ,  Karthik N. Muthuraman ,  Jeffrey Beringer ,  Sara Bennett ,  Huixia Wu ,  Shavell Gorman ,  Andrew Worden

IPC分类号： C12N15/113 ,  C12N15/82

摘要： Provided are vector constructs and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements, including the promoters, 5′-UTRs, and/or 3′-UTRs, isolated from Zea mays.

公开(公告)号：US20130198898A1

公开(公告)日：2013-08-01

申请号：US13674712

申请日：2012-11-12

申请人： Dow AgroSciences LLC

发明人： Sandeep Kumar ,  Diaa Alabed ,  Sara Bennett ,  Manju Gupta ,  Susan Jayne ,  Terry R. Wright

IPC分类号： C12N15/82

CPC分类号： C12N15/8216 ,  C12N15/8286 ,  Y02A40/162

摘要： Provided are constructs and methods for expressing multiple genes in plant cells and/or plant tissues. The constructs provided comprise at least one bi-directional promoter linked to multiple gene expression cassettes, wherein the bi-directional promoter comprises a functional promoter nucleotide sequence from Sugar Cane Bacilliform Virus promoter. In some embodiments, the constructs and methods provided employs a bi-directional promoter based on a minimal core promoter element from a Zea mays Ubiquitin-1 gene, or a functional equivalent thereof, and nucleotide sequence elements from a Sugar Cane Bacilliform Virus promoter. In some embodiments, the constructs and methods provided allow expression of genes between three and twenty.

摘要翻译： 提供了用于在植物细胞和/或植物组织中表达多个基因的构建体和方法。 所提供的构建体包括至少一个与多个基因表达盒连接的双向启动子，其中双向启动子包含来自甘蔗芽孢杆菌病毒启动子的功能启动子核苷酸序列。 在一些实施方案中，所提供的构建体和方法采用基于来自玉米泛素-1基因或其功能等同物的最小核心启动子元件和来自甘蔗芽孢杆菌病毒启动子的核苷酸序列元件的双向启动子。 在一些实施方案中，提供的构建体和方法允许在3至20个之间表达基因。

公开(公告)号：US10047368B2

公开(公告)日：2018-08-14

申请号：US14514837

申请日：2014-10-15

申请人： Dow AgroSciences LLC

发明人： Manju Gupta ,  Navin Elango ,  Karthik N. Muthuraman ,  Jeffrey Beringer ,  Sara Bennett ,  Huixia Wu

IPC分类号： C12N15/82 ,  C12N15/113

摘要： Provided are vector constructs and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements, including the promoters, 5′-UTRs, introns, and/or 3′-UTRs, isolated from Zea mays.

公开(公告)号：US20170081676A1

公开(公告)日：2017-03-23

申请号：US15266370

申请日：2016-09-15

申请人： Dow AgroSciences LLC

发明人： Manju Gupta ,  Sara Bennett ,  Andrew F. Worden

IPC分类号： C12N15/82

CPC分类号： C12N15/8216 ,  C12N15/8222 ,  C12N15/8274 ,  C12N15/8286 ,  Y02A40/162

摘要： This disclosure concerns compositions and methods for promoting transcription and translation of a nucleotide sequence in a plant or plant cell, employing a 3′UTR from Arabidopsis thaliana Ubiquitin-10 gene. Some embodiments relate to a 3′ UTR from a Arabidopsis thaliana Ubiquitin-10 gene that functions in plants to terminate transcription of operably linked nucleotide sequences.

公开(公告)号：US09453235B2

公开(公告)日：2016-09-27

申请号：US13674712

申请日：2012-11-12

申请人： Dow AgroSciences LLC

发明人： Sandeep Kumar ,  Diaa Alabed ,  Sara Bennett ,  Manju Gupta ,  Susan Jayne ,  Terry R. Wright

IPC分类号： C12N15/82

CPC分类号： C12N15/8216 ,  C12N15/8286 ,  Y02A40/162

摘要： Provided are constructs and methods for expressing multiple genes in plant cells and/or plant tissues. The constructs provided comprise at least one bi-directional promoter linked to multiple gene expression cassettes, wherein the bi-directional promoter comprises a functional promoter nucleotide sequence from Sugar Cane Bacilliform Virus promoter. In some embodiments, the constructs and methods provided employs a bi-directional promoter based on a minimal core promoter element from a Zea mays Ubiquitin-1 gene, or a functional equivalent thereof, and nucleotide sequence elements from a Sugar Cane Bacilliform Virus promoter. In some embodiments, the constructs and methods provided allow expression of genes between three and twenty.

摘要翻译： 提供了用于在植物细胞和/或植物组织中表达多个基因的构建体和方法。 所提供的构建体包括至少一个与多个基因表达盒连接的双向启动子，其中双向启动子包含来自甘蔗芽孢杆菌病毒启动子的功能启动子核苷酸序列。 在一些实施方案中，所提供的构建体和方法采用基于来自玉米泛素-1基因或其功能等同物的最小核心启动子元件和来自甘蔗芽孢杆菌病毒启动子的核苷酸序列元件的双向启动子。 在一些实施方案中，提供的构建体和方法允许在3至20个之间表达基因。

公开(公告)号：US20170081675A1

公开(公告)日：2017-03-23

申请号：US15266348

申请日：2016-09-15

申请人： Dow AgroSciences LLC

发明人： Manju Gupta ,  Sara Bennett ,  Andrew F. Worden

IPC分类号： C12N15/82

CPC分类号： C12N15/8216 ,  C07K14/415 ,  C12N15/8205 ,  C12N15/8222 ,  C12N15/8241 ,  C12N15/8273 ,  C12N15/8274 ,  C12N15/8286

摘要： This disclosure concerns compositions and methods for promoting transcription and translation of a nucleotide sequence in a plant or plant cell, employing a 3′UTR from Arabidopsis thaliana Ubiquitin C9 gene. Some embodiments relate to a 3′ UTR from a Arabidopsis thaliana Ubiquitin C9 gene that functions in plants to terminate transcription of operably linked nucleotide sequences.

公开(公告)号：US11236350B2

公开(公告)日：2022-02-01

申请号：US16139112

申请日：2018-09-24

申请人： Dow AgroSciences LLC

发明人： Sandeep Kumar ,  Sara Bennett ,  Andrew J. Bowling ,  Alicia Walker ,  Cory M. Larsen ,  Heather Pence ,  Staci Weaver ,  Ronnie Hampton, Jr.

IPC分类号： C12N15/82 ,  A01H6/60

摘要： This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a promoter from a Glycine max egg cell gene. Some embodiments relate to a promoter or a 5′ UTR from a Glycine max egg cell gene that functions in plants to promote transcription of operably linked nucleotide sequences. Other embodiments relate to a 3′ UTR or a terminator from a Glycine max egg cell gene that functions in plants to promote transcription of operably linked nucleotide sequences.

公开(公告)号：US10294485B2

公开(公告)日：2019-05-21

申请号：US15266348

申请日：2016-09-15

申请人： Dow AgroSciences LLC

发明人： Manju Gupta ,  Sara Bennett ,  Andrew F. Worden

IPC分类号： C12N15/82 ,  C07K14/415

摘要： This disclosure concerns compositions and methods for promoting transcription and translation of a nucleotide sequence in a plant or plant cell, employing a 3′UTR from Arabidopsis thaliana Ubiquitin C9 gene. Some embodiments relate to a 3′ UTR from a Arabidopsis thaliana Ubiquitin C9 gene that functions in plants to terminate transcription of operably linked nucleotide sequences.

公开(公告)号：US09650641B2

公开(公告)日：2017-05-16

申请号：US14604510

申请日：2015-01-23

申请人： Dow AgroSciences LLC

发明人： Manju Gupta ,  Sara Bennett ,  Navin Elango ,  Karthik Muthuraman ,  Jeffrey Beringer ,  Huixia Wu

IPC分类号： C12N15/82

CPC分类号： C12N15/8223 ,  C12N15/8218 ,  C12N15/8271 ,  C12N15/8279

摘要： Provided are constructs and methods for expressing a transgene in plant cells and/or plant tissues using Zea mays chlorophyll a/b binding gene regulatory elements.

公开(公告)号：US20190100764A1

公开(公告)日：2019-04-04

申请号：US16139112

申请日：2018-09-24

申请人： Dow AgroSciences LLC

发明人： Sandeep Kumar ,  Sara Bennett ,  Andrew J. Bowling ,  Alicia Walker ,  Cory M. Larsen ,  Heather Pence ,  Staci Weaver ,  Ronnie Hampton, JR.

IPC分类号： C12N15/82

摘要： This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a promoter from a Glycine max egg cell gene. Some embodiments relate to a promoter or a 5′ UTR from a Glycine max egg cell gene that functions in plants to promote transcription of operably linked nucleotide sequences. Other embodiments relate to a 3′ UTR or a terminator from a Glycine max egg cell gene that functions in plants to promote transcription of operably linked nucleotide sequences.