公开(公告)号：US20170212112A1

公开(公告)日：2017-07-27

申请号：US15005054

申请日：2016-01-25

Applicant: General Electric Company

Inventor： Bing Li ,  Matthew Jeremiah Misner ,  Gregory Andrew Grossmann ,  Cathryn Ellen Olsen ,  Arunkumar Natarajan

IPC: G01N33/569

CPC classification number: G01N33/56905 ,  G01N33/54393 ,  G01N2333/445

Abstract: Provided herein are integrated devices that improve sensitivity of rapid diagnostic tests. The devices described herein integrate sample separation with rapid diagnostic tests and improve signal intensity of the rapid diagnostic tests.

公开(公告)号：US09708349B2

公开(公告)日：2017-07-18

申请号：US14621776

申请日：2015-02-13

Applicant: GENERAL ELECTRIC COMPANY

Inventor： Arunkumar Natarajan ,  Anup Sood ,  Lakshmi Sireesha Kaanumalle

IPC: C07F5/02 ,  C07F9/02

CPC classification number: C07F5/02

Abstract: A borate compound for use as photoinduced chemical bleaching reagent is disclosed having improved bleaching performance under certain conditions, compared to neutral borates. The compounds are comprised of Formula I; R1R2R3R4BXa   I where each R1, R2, and R3 and R4 is, independently, an alkyl, alkylaryl, or arylalkyl, with the proviso that at least one of R1, R2, and R3 and R4 is an alkyl group and at least two of R1, R2, R3 and R4 group is positively charged; X is an anion; and a equals 1, 2, or 3.

公开(公告)号：US09322051B2

公开(公告)日：2016-04-26

申请号：US14047233

申请日：2013-10-07

Applicant: GENERAL ELECTRIC COMPANY

Inventor： Anup Sood ,  Arunkumar Natarajan ,  Michael James Rishel ,  Michael John Gerdes

IPC: C12Q1/68 ,  G01N33/68 ,  G01N33/53 ,  G01N33/533 ,  G01N33/542 ,  G01N33/543

CPC classification number: C12Q1/6825 ,  C12Q2523/319 ,  G01N33/53 ,  G01N33/533 ,  G01N33/542 ,  G01N33/6854

Abstract: Disclosed are high throughput methods of probing multiple targets in a biological sample where the recurrent time-consuming antibody incubation steps and individual signal modification and activation steps are replaced by simultaneous hybridization of biomarkers and sequential detection by combining signal removal and activation into a single step. Also disclosed are images obtained by such methods.

Abstract translation: 公开了探测生物样品中多个靶标的高通量方法，其中复发耗时的抗体孵育步骤和单独的信号修饰和激活步骤被生物标志物的同时杂交和通过将信号去除和活化组合到单个步骤中的顺序检测来代替。 还公开了通过这种方法获得的图像。

公开(公告)号：US09176032B2

公开(公告)日：2015-11-03

申请号：US13786747

申请日：2013-03-06

Applicant: General Electric Company

Inventor： Anup Sood ,  Kevin Bernard Kenny ,  Arunkumar Natarajan ,  Lakshmi Sireesha Kaanumalle

IPC: G01N33/53 ,  G01N33/532 ,  G01N33/533 ,  G01N21/76 ,  G01N1/30 ,  G01N33/58 ,  G01N21/64

CPC classification number: G01N1/30 ,  G01N21/64 ,  G01N21/6428 ,  G01N33/582

Abstract: Methods comprising the use probing multiple targets in a H&E stained biological sample are provided. The methods include the steps of providing a hematoxylin and eosin stained biological sample containing multiple targets, observing the sample, removing the hematoxylin and partially removing the eosin by washing the sample, contacting the sample with a borate salt, and irradiating the sample to remove the residual eosin fluorescence. The method further includes the optionally performing the additional steps of binding at least one probe to one or more targets to the sample, observing a signal from the probe and contacting the sample with a bleaching agent. The process of binding, observing and bleaching may be iteratively repeated.

Abstract translation: 提供了包括在H＆E染色的生物样品中使用探测多个靶标的方法。 所述方法包括以下步骤：提供含有多个靶标的苏木精和伊红染色的生物样品，观察样品，除去苏木精并通过洗涤样品部分除去曙红，使样品与硼酸盐接触，并照射样品以除去 残余曙红荧光。 该方法还包括任选地执行将至少一个探针结合至样品的一个或多个靶的附加步骤，观察来自探针的信号并使样品与漂白剂接触。 结合，观察和漂白的过程可以迭代地重复。

公开(公告)号：US20130178392A1

公开(公告)日：2013-07-11

申请号：US13786747

申请日：2013-03-06

Applicant: General Electric Company

Inventor： Anup Sood ,  Kevin Bernard Kenny ,  Arunkumar Natarajan ,  Lakshmi Sireesha Kaanumalle

IPC: G01N1/30

CPC classification number: G01N1/30 ,  G01N21/64 ,  G01N21/6428 ,  G01N33/582

Abstract: Methods comprising the use probing multiple targets in a H&E stained biological sample are provided. The methods include the steps of providing a hematoxylin and eosin stained biological sample containing multiple targets, observing the sample, removing the hematoxylin and partially removing the eosin by washing the sample, contacting the sample with a borate salt, and irradiating the sample to remove the residual eosin fluorescence. The method further includes the optionally performing the additional steps of binding at least one probe to one or more targets to the sample ,observing a signal from the probe and contacting the sample with a bleaching agent. The process of binding, observing and bleaching may be iteratively repeated.

Abstract translation: 提供了包括在H＆E染色的生物样品中使用探测多个靶标的方法。 所述方法包括以下步骤：提供含有多个靶标的苏木精和伊红染色的生物样品，观察样品，除去苏木精并通过洗涤样品部分除去曙红，使样品与硼酸盐接触，并照射样品以除去 残余曙红荧光。 该方法还包括任选地执行将至少一个探针结合至样品的一个或多个靶的附加步骤，观察来自探针的信号并使样品与漂白剂接触。 结合，观察和漂白的过程可以迭代地重复。