Method of analyzing 5-hydroxyindoles and catecholamines, and a device
for performing the same
    52.
    发明授权
    Method of analyzing 5-hydroxyindoles and catecholamines, and a device for performing the same 失效
    分析5-羟基吲哚和儿茶酚胺的方法及其用途

    公开(公告)号:US5824559A

    公开(公告)日:1998-10-20

    申请号:US664909

    申请日:1996-06-17

    摘要: A method and a device for the analysis of 5-hydroxyindoles or catecholamines with high sensitivity. New chemiluminescence labeling agents, 6-aminomethylphthalhydrazide or 1,2-bis(phthalhydrazino)ethylenediamine, are reacted with 5-hydroxyindoles or catecholamines to form their stable derivatives. The derivatives emit strong luminescence in the presence of an oxidizing agent. In the chemiluminescence detection method, there is extremely low background noise and thus the method enables analysis with high sensitivity. The analysis of 5-hydroxyindoles or catecholamines in a sample is enabled by a method (1), in which each of the above-mentioned components is separated, reacted with said chemiluminescence labeling agent to form its derivative, and then chemiluminescent emitted by the reaction of the derivative with an oxidizing agent is detected, or by another method (2), in which the mixture of 5-hydroxyindoles or catecholamines in a sample is reacted with the chemiluminescence labeling agent to form its derivative mixture, subsequently each derivative is separated, and then chemiluminescent emitted by the reaction of the derivative with an oxidizing agent is detected.

    摘要翻译: 用于分析高灵敏度的5-羟基吲哚或儿茶酚胺的方法和装置。 新化学发光标记试剂,6-氨基甲基邻苯二甲酰肼或1,2-双(邻苯二甲酰肼基)乙二胺与5-羟基吲哚或儿茶酚胺反应形成其稳定的衍生物。 衍生物在氧化剂存在下发出强烈的发光。 在化学发光检测方法中,具有极低的背景噪声,因此该方法能够以高灵敏度进行分析。 通过方法(1)可以分析样品中的5-羟基吲哚或儿茶酚胺,其中将上述各成分分离,与所述化学发光标记试剂反应形成其衍生物,然后由反应发出化学发光 或通过另一种方法(2),其中将样品中的5-羟基吲哚或儿茶酚胺的混合物与化学发光标记试剂反应以形成其衍生物混合物,随后分离每种衍生物, 然后检测通过衍生物与氧化剂的反应发射的化学发光。