公开(公告)号：US20090218514A1

公开(公告)日：2009-09-03

申请号：US11720842

申请日：2005-12-07

申请人： Derk Jan Wilfred Klunder ,  Maarten Van Herpen ,  Marcello Balistreri ,  Coen Liedenbaum ,  Menno Prins ,  Reinhold Wimberger-Friedl ,  Ralph Kurt

发明人： Derk Jan Wilfred Klunder ,  Maarten Van Herpen ,  Marcello Balistreri ,  Coen Liedenbaum ,  Menno Prins ,  Reinhold Wimberger-Friedl ,  Ralph Kurt

IPC分类号： G01J1/58

CPC分类号： G01N21/6456 ,  G01N21/6452 ,  G01N21/648 ,  G01N2021/1772 ,  G01N2021/6463 ,  G01N2021/6478 ,  G01N2201/0461

摘要： The invention relates to a method and an apparatus for the investigation of a sample material by multiple sample light spots (501) generated by evanescent waves. An array of source light spots (510) is generated by a multi-spot generator, e.g. a multi-mode interferometer (106), and mapped onto sample light spots (501) in a sample layer (302) by (micro-)lenses (202, 203) or by the Talbot effect. The input light (504) of the source light spots (510) is shaped such that all of it is totally internally reflected at the interface between a transparent carrier plate (301) and the sample layer (302). Thus the sample light spots (501) consist of evanescent waves only and are restricted to a limited volume. In a preferred application, fluorescence stimulated in the sample light spots (501) is detected with spatial resolution by a CCD array (401).

摘要翻译： 本发明涉及一种通过由ev逝波产生的多个样本光点（501）来研究样品材料的方法和装置。 源光点阵列（510）由多点发生器产生，例如， 多模干涉仪（106），并且通过（微）透镜（202,203）或通过Talbot效应将样品层（302）中的样品光点（501）映射。 源光点（510）的输入光（504）被成形为使得它们全部在内部被透明载体板（301）和样品层（302）之间的界面反射。 因此，样本光点（501）仅由ev逝波组成，并被限制在有限的体积。 在优选的应用中，通过CCD阵列（401）以空间分辨率检测样品光点（501）中刺激的荧光。

公开(公告)号：US07558446B2

公开(公告)日：2009-07-07

申请号：US12089939

申请日：2006-10-10

申请人： Reinhold Wimberger-Friedl ,  Gert 'T Hooft ,  Christiane De Witz

发明人： Reinhold Wimberger-Friedl ,  Gert 'T Hooft ,  Christiane De Witz

IPC分类号： G02B6/00

CPC分类号： G01N21/7703 ,  G01N21/648

摘要： The present invention is directed to an evanescent field induced optical sensor of a transparent polymer substrate coated with a thin transparent polymer waveguide for use as biosensor for detecting biological molecules, wherein the transparent polymer waveguide has a thickness of ≧0.10 μm and ≦0.50 μm and a refractive index nD of 1.39 to 1.79, said transparent polymer waveguide has a coupling grating recess structure for enhancing the coupling of the light wave into said transparent polymer waveguide, the lower surface of said transparent polymer waveguide contacts the transparent polymer substrate, said transparent polymer substrate has a refractive index n of 1.29 to 1.69, wherein the material of said transparent polymer waveguide and said transparent polymer substrate is selected such that the difference of the refractive index ΔnD of said transparent polymer waveguide and said transparent polymer substrate is at least Δ n 0.1, the outer upper surface of said polymer waveguide possess specific binding compounds to detect at least one specific chemical and/or biochemical substance or the outer upper surface of said polymer waveguide is covered with a thin noble metal layer.

摘要翻译： 本发明涉及涂覆有用作检测生物分子的生物传感器的薄透明聚合物波导的透明聚合物基质的消逝场感应光学传感器，其中透明聚合物波导的厚度>0.10μm和<= 0.50 所述透明聚合物波导具有用于增强所述光波与所述透明聚合物波导耦合的耦合光栅凹槽结构，所述透明聚合物波导的下表面与透明聚合物基底接触，所述透明聚合物波导 透明聚合物基材的折射率n为1.29〜1.69，其中所述透明聚合物波导和所述透明聚合物基材的材料选择为使得所述透明聚合物波导和所述透明聚合物基材的折射率DeltanD的差异至少为 Δn0.1，所述聚合物的外部上表面 veguide具有特异性结合化合物以检测至少一种特定化学和/或生物化学物质，或者所述聚合物波导的外部上表面被薄的贵金属层覆盖。

公开(公告)号：US20080257071A1

公开(公告)日：2008-10-23

申请号：US12094849

申请日：2006-11-16

申请人： Reinhold Wimberger-Friedl ,  Christiane De Witz

发明人： Reinhold Wimberger-Friedl ,  Christiane De Witz

IPC分类号： G01N1/00

CPC分类号： B01L3/5027 ,  B01D2313/086 ,  B01L3/502753 ,  B01L2300/0636 ,  B01L2300/0681 ,  B01L2300/0861 ,  B01L2300/0887

摘要： The invention relates to a microfluidic device for detection of a substance in a sample fluid, and to a cartridge for performing a biological assay, containing such a device. The microfluidic device comprises two housing parts (52, 54) with a porous membrane (50) there between. Each housing part has recesses, or channel parts, (56-1, 56-2, 56-n, 58-1, 58-2, 58-n) that are connected via a recess of the opposite housing part, and through the membrane (50), such that an unbranched channel is defined for the sample fluid. At one or more of the positions where the channel crosses the membrane (50), a spot (48-1, 48-2, 48-n) with an immobilized indicator substance is present, to which a target substance in the sample fluid may bind. An advantage of the present device is that in principle all of the sample fluid passes each spot. Hence there is no need to recirculate and/or mix the sample fluid, as is the case in devices with parallel flow-through paths for the fluid. The device will therefore be simpler, and give a more reliable detection result.

摘要翻译： 本发明涉及用于检测样品流体中的物质的微流体装置和用于进行包含这种装置的生物测定的盒。 微流体装置包括两个在其间具有多孔膜（50）的壳体部分（52,54）。 每个壳体部分具有经由相对的壳体部分的凹部连接的凹部或通道部分（56-1,56-2,66-n，58-1,58-2,58-n），并且通过 膜（50），使得为样品流体定义未分支的通道。 在通道穿过膜（50）的一个或多个位置处，存在具有固定的指示物质的斑点（48-1,48-2,48-n），样品流体中的目标物质可以 绑定 本装置的优点在于，原则上所有样品流体都通过每个点。 因此，不需要再循环和/或混合样品流体，如同用于流体的平行流通路径的装置的情况。 因此，该装置将更简单，并且给出更可靠的检测结果。

公开(公告)号：US20070097314A1

公开(公告)日：2007-05-03

申请号：US10582578

申请日：2004-12-10

申请人： Reinhold Wimberger-Friedl ,  Johan De Bruin ,  Emile Verstegen

发明人： Reinhold Wimberger-Friedl ,  Johan De Bruin ,  Emile Verstegen

IPC分类号： G02C7/02

CPC分类号： G03F7/001 ,  C08L33/04 ,  C08L63/00 ,  C08L2314/00 ,  G02B1/041 ,  C08L2666/22

摘要： The invention relates to a process for the fabrication of a polymeric optical microstructure, being supported or not by a substrate, starting from a thermoplastic polymer, wherein a thermoplastic polymer is blended with an UV curable resin and a thermally stable photo-initiator, to obtain a blend having a lower viscosity than the viscosity of said polymer, said blend being molded and the molded blend being cured by means of UV radiation to obtain a polymeric optical micro structure. Such a process prevents the common problems, which arise with molding of conventional thermoplastic polymers and conventional UV curing when only one of the components of the blend is used.

摘要翻译： 本发明涉及从热塑性聚合物开始制造聚合物光学微结构的方法，由聚合物支撑或不被基底支撑，其中热塑性聚合物与紫外光固化树脂和热稳定的光引发剂共混以获得 具有比所述聚合物的粘度低的粘度的共混物，所述共混物被模制，并且所述模制共混物通过UV辐射固化以获得聚合物光学微结构。 这种方法防止了当仅使用共混物的一种成分时常规热塑性聚合物的成型和常规的UV固化而产生的常见问题。