公开(公告)号：US4496658A

公开(公告)日：1985-01-29

申请号：US311327

申请日：1981-10-14

Applicant: Koichi Kondo ,  Susumu Iwasa ,  Isamu Yoshida

Inventor： Koichi Kondo ,  Susumu Iwasa ,  Isamu Yoshida

IPC: C07K14/59 ,  C07K16/26 ,  C12N9/96 ,  G01N33/531 ,  G01N33/535 ,  G01N33/543 ,  G01N33/76 ,  G01N33/54 ,  G01N33/56 ,  G01N33/58

CPC classification number: C07K14/59 ,  C07K16/26 ,  C12N9/96 ,  G01N33/531 ,  G01N33/535 ,  G01N33/54306 ,  G01N33/76 ,  Y10S435/975 ,  Y10S436/80 ,  Y10S436/804 ,  Y10S436/808 ,  Y10S436/813 ,  Y10S436/814 ,  Y10S436/819 ,  Y10S930/11 ,  Y10S930/28

Abstract: In a method for immunochemical assay of human chorionic gonadotropin involving use of an antibody immobilized on a carrier, an antigen and an antibody to which a labeling agent has been attached, when the antibody supported on the carrier and the antibody coupled to a labeling agent are different antibodies which are not overlapping in antigen-determining site and one of said different antibodies is specifically reactive to human chorionic gonadotropin, a high reproducibility of the result of the immunochemical assay is obtained.

Abstract translation: 在涉及使用固定在载体上的抗体，附着有标记试剂的抗原和抗体的人绒毛膜促性腺激素的免疫化学测定方法中，当载体上载有抗体和与标记试剂偶联的抗体时， 在抗原决定部位和所述不同抗体之一中不重叠的不同抗体与人绒毛膜促性腺激素特异性反应，获得了免疫化学测定结果的高再现性。