公开(公告)号：US20110111424A1

公开(公告)日：2011-05-12

申请号：US12967284

申请日：2010-12-14

申请人： John Edward Rush, II ,  Jing Li ,  Ailan Guo

发明人： John Edward Rush, II ,  Jing Li ,  Ailan Guo

IPC分类号： G01N33/53 ,  C07K16/00

CPC分类号： C07K16/00 ,  G01N33/6842 ,  G01N2440/36

摘要： The invention relates to antibody reagents that specifically bind to peptides carrying a ubiquitin remnant from a digested or chemically treated biological sample. The reagents allow the technician to identify ubiquitinated polypeptides as well as the sites of ubiquitination on them. The reagents are preferably employed in proteomic analysis using mass spectrometry. The antibody reagents specifically bind to the remnant of ubiquitin (i.e., a diglycine modified epsilon amine of lysine) left on a peptide which as been generated by digesting or chemically treating ubiquitinated proteins. The inventive antibody reagents' affinity to the ubiquitin remnant does not depend on the remaining amino acid sequences flanking the modified (i.e., ubiquitinated) lysine, i.e., they are context independent.

摘要翻译： 本发明涉及与经消化或经化学处理的生物样品中携带泛素残基的肽特异性结合的抗体试剂。 试剂允许技术人员识别泛素化的多肽以及它们上泛素化的位点。 试剂优选用于使用质谱的蛋白质组学分析。 抗体试剂特异性结合残留在通过消化或化学处理泛素化蛋白质产生的肽上的泛素（即，赖氨酸的二甘氨酸修饰的ε胺）残基。 本发明的抗体试剂对泛素残留物的亲和力不依赖于修饰（即泛素化）赖氨酸侧翼的剩余氨基酸序列，即它们是上下文无关的。

公开(公告)号：US20090203043A1

公开(公告)日：2009-08-13

申请号：US12313571

申请日：2008-11-21

申请人： Peter Hornbeck ,  Ailan Guo

发明人： Peter Hornbeck ,  Ailan Guo

IPC分类号： G01N33/573 ,  C07K16/18 ,  G01N33/566

CPC分类号： G01N33/68 ,  G01N2333/4703 ,  G01N2333/91215

摘要： The invention discloses 142 novel phosphorylation sites identified in insulin signaling pathways, peptides (including AQUA peptides) comprising a phosphorylation site of the invention, antibodies specifically bind to a novel phosphorylation site of the invention, and diagnostic and therapeutic uses of the above.

摘要翻译： 本发明公开了在胰岛素信号通路中鉴定的142个新的磷酸化位点，包含本发明的磷酸化位点的肽（包括AQUA肽），特异性结合本发明的新的磷酸化位点的抗体以及上述的诊断和治疗用途。

公开(公告)号：US06495737B1

公开(公告)日：2002-12-17

申请号：US08909125

申请日：1997-08-11

申请人： Daniel F. Klessig ,  Ailan Guo

发明人： Daniel F. Klessig ,  Ailan Guo

IPC分类号： C12N1582

CPC分类号： C12N15/8279 ,  C12N9/14

摘要： The present invention provides methods and materials that enhance a plant's resistance to certain pathogens. A novel pathway is described and has been designated with the acronym, SI-SAR pathway, for salicylic acid-independent systemic acquired resistance. DNA constructs and methodologies are provided that facilitate the identification of compounds that activate this pathway. Methods are provided to enable the identification of novel genes and signaling components that are expressed when the SI-SAR pathway is activated. Transgenic plants with altered expression of these novel genes or signaling components of the pathway are expected to have enhanced resistance to plant pathogens. Also provided is a novel, pathogen-induced epoxide hydrolase that is inducible in the absence of SA.

摘要翻译： 本发明提供增强植物对某些病原体的抗性的方法和材料。 描述了一条新途径，并且已经用缩写SI-SAR途径指定了水杨酸独立系统获得性抗性。 提供DNA构建体和方法，其有助于鉴定激活该途径的化合物。 提供了方法来确定当SI-SAR途径被激活时表达的新基因和信号传导成分。 预期这些新基因或该途径的信号成分表达改变的转基因植物对植物病原体具有增强的抗性。 还提供了一种新型的病原体诱导的环氧化物水解酶，其在不存在SA时可诱导。