利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

65 条记录 (耗时 0.05 秒)

    Method for determining depression, kit for analyzing serotonin transporter, and kit for analyzing ubiquitinated serotonin transporter in blood
    3.
    发明授权
    Method for determining depression, kit for analyzing serotonin transporter, and kit for analyzing ubiquitinated serotonin transporter in blood 有权
    用于测定抑郁症的方法，用于分析血清素转运蛋白的试剂盒和用于分析血液中的泛素化血清素转运蛋白的试剂盒

    公开(公告)号：US09274127B2

    公开(公告)日：2016-03-01

    申请号：US14233374

    申请日：2012-07-19

    申请人： Toshitaka Nabeshima ,  Akihiro Mouri

    发明人： Toshitaka Nabeshima ,  Akihiro Mouri

    IPC分类号： G01N31/00 ,  G01N33/53 ,  G01N33/68 ,  G01N33/94 ,  C12Q1/68

    CPC分类号： G01N33/942 ,  C12Q1/6881 ,  G01N33/6896 ,  G01N2333/4703 ,  G01N2440/36 ,  G01N2800/304

    摘要： [Problem] To provide: a method for utilizing a novel marker, including a method for determining depression; and a kit for analyzing an ubiquitinated serotonin transporter.[Solution] A method for determining depression, comprising a step of analyzing the proportion of an ubiquitinated serotonin transporter in a blood sample collected from a subject; and a kit for analyzing an ubiquitinated serotonin transporter in blood, which comprises an ubiquitinated protein collector material and an anti-serotonin transporter antibody and is used for the analysis of the proportion of an ubiquitinated serotonin transporter in a collected blood sample.

    摘要翻译： [问题]提供：一种利用新型标记的方法，包括用于确定抑郁症的方法; 以及用于分析泛素化的5-羟色胺转运蛋白的试剂盒。 [解决方案]一种用于测定抑郁症的方法，包括分析从受试者收集的血液样品中的遍在蛋白化的5-羟色胺转运蛋白的比例的步骤; 以及用于分析血液中泛素化的血清素转运蛋白的试剂盒，其包含泛素化的蛋白质收集剂材料和抗5-羟色胺转运蛋白抗体，并用于分析收集的血液样品中普遍存在的5-羟色胺转运蛋白的比例。

    DIAGNOSTIC METHOD BASED ON LARGE SCALE IDENTIFICATION OF POST-TRANSLATIONAL MODIFICATION OF PROTEINS
    4.
    发明申请
    DIAGNOSTIC METHOD BASED ON LARGE SCALE IDENTIFICATION OF POST-TRANSLATIONAL MODIFICATION OF PROTEINS 审中-公开
    基于大规模鉴定蛋白质翻译后修饰的诊断方法

    公开(公告)号：US20150309044A1

    公开(公告)日：2015-10-29

    申请号：US14796408

    申请日：2015-07-10

    申请人： President and Fellows of Harvard College

    发明人： Yifat Merbl ,  Marc W. Kirschner

    IPC分类号： G01N33/68

    CPC分类号： G01N33/6845 ,  G01N33/57484 ,  G01N33/6842 ,  G01N2440/00 ,  G01N2440/14 ,  G01N2440/36 ,  G01N2800/00 ,  G01N2800/28 ,  G01N2800/2821 ,  G01N2800/2835

    摘要： Methods for the large scale identification of post-translational modification states of proteins and enzyme activities for carrying out post-translational modification reactions involve the analysis of functional extracts from fresh and frozen samples using protein arrays. The methods and kits of the present invention can be used to analyze and characterize compounds for their effects on post-translational modifications and their pathways. The methods and kits can also be used to diagnose and characterize a wide variety of diseases and medical conditions, including cancer, neurodegenerative diseases, immune diseases, infectious diseases, genetic diseases, metabolic conditions, and drug effects using cells or body fluids of a patient.

    摘要翻译： 用于进行翻译后修饰反应的大规模鉴定蛋白质和酶活性的翻译后修饰状态的方法涉及使用蛋白质阵列分析来自新鲜和冷冻样品的功能提取物。 本发明的方法和试剂盒可用于分析和表征化合物对其翻译后修饰及其途径的影响。 方法和试剂盒还可用于诊断和表征各种疾病和医学状况，包括癌症，神经变性疾病，免疫疾病，传染病，遗传疾病，代谢状况和使用患者的细胞或体液的药物作用 。

    METHODS AND COMPOSITIONS FOR INHIBITING NEDDYLATION OF PROTEINS
    6.
    发明申请
    METHODS AND COMPOSITIONS FOR INHIBITING NEDDYLATION OF PROTEINS 审中-公开
    用于抑制蛋白质的方法和组合物

    公开(公告)号：US20140179593A1

    公开(公告)日：2014-06-26

    申请号：US14118132

    申请日：2012-05-16

    申请人： Julie K. Monda ,  Brenda A. Schulman ,  Daniel C. Scott

    发明人： Julie K. Monda ,  Brenda A. Schulman ,  Daniel C. Scott

    IPC分类号： C07K14/47 ,  G01N33/566

    CPC分类号： C07K14/47 ,  A61K38/00 ,  C07K2299/00 ,  C12N9/93 ,  C12Y603/02019 ,  G01N33/566 ,  G01N33/573 ,  G01N2333/9015 ,  G01N2440/36 ,  G01N2500/04

    摘要： Provided herein is a novel binding pocket within NEDD8 co-E3 proteins that binds NEDD8 E2 enzymes. Particularly at its M-Terminus. Methods are provided for screening for compounds that bind to the disclosed E2-binding pocket in NEDD8 co-E3 proteins. Compounds that bind to the E2-binding pocket and optionally inhibit the activity of NEDD8 co-E3 proteins and pharmaceutical compositions comprising the same are further provided. The NEDD8 co-E3 inhibitors find use, as agents preventing the NEDDylation of a target protein, in inhibiting cell growth and methods for treating cancers, inflammatory disorders, and pathogenic infections. The preferred inhibitors are peptides corresponding to a M-terminal fragment of Dnc1, e.g. MTLASKLKRDD, MLKLRQLQKKKQ, and MIKLFSLKQQKK, which are substituted at the M-Terminus with an uncharged group (e.g. acyl).

    摘要翻译： 本文提供了结合NEDD8 E2酶的NEDD8 co-E3蛋白内的新结合口袋。 特别是在其M-Terminus。 提供了用于筛选与NEDD8共-E3蛋白中公开的E2结合口袋结合的化合物的方法。 还提供结合E2结合口袋并任选地抑制NEDD8共-E3蛋白的活性的化合物和包含其的药物组合物。 NEDD8 co-E3抑制剂用作抑制靶蛋白的NEDD化作为抑制细胞生长的药剂以及用于治疗癌症，炎性疾病和致病性感染的方法。 优选的抑制剂是对应于Dnc1的M末端片段的肽，例如 MTLASKLKRDD，MLKLRQLQKKKQ和MIKLFSLKQQKK，它们在不带电荷的基团（例如酰基）的M-Terminus取代。

    METHODS AND KITS FOR DIAGNOSING SJOGREN'S SYNDROME
    7.
    发明申请
    METHODS AND KITS FOR DIAGNOSING SJOGREN'S SYNDROME 有权
    用于诊断SJOGREN'S SYNDROME的方法和工具

    公开(公告)号：US20140134644A1

    公开(公告)日：2014-05-15

    申请号：US14078020

    申请日：2013-11-12

    申请人： The General Hospital Corporation

    发明人： Denise L. FAUSTMAN

    IPC分类号： G01N33/543

    CPC分类号： G01N33/564 ,  G01N2333/912 ,  G01N2440/14 ,  G01N2440/36 ,  G01N2800/101

    摘要： The invention features methods and kits for determining the presence of, or a predisposition to develop, Sjögren's syndrome in humans. The invention features methods to detect changes in the levels of one or more LMP-2 protein isoforms, in particular phosphorylated isoforms of LMP-2, and to detect changes in cellular protein phosphorylation and ubiquitination in samples from Sjögren's patients.

    摘要翻译： 本发明的特征在于确定人类Sjögren综合征的存在或发生倾向的方法和试剂盒。 本发明的特征在于检测一种或多种LMP-2蛋白质异构体（特别是LMP-2的磷酸化同种型）的水平变化的方法，并检测Sjögren患者样品中细胞蛋白磷酸化和泛素化的变化。

    Detection and quantification of modified proteins
    8.
    发明申请
    Detection and quantification of modified proteins 审中-公开
    修饰蛋白的检测和定量

    公开(公告)号：US20120149883A1

    公开(公告)日：2012-06-14

    申请号：US13284255

    申请日：2011-10-28

    申请人： Steven P. Gygi ,  Junmin Peng

    发明人： Steven P. Gygi ,  Junmin Peng

    IPC分类号： C07K17/00 ,  C07K16/00

    CPC分类号： G01N33/6848 ,  C07K1/22 ,  C07K7/08 ,  C12Q1/37 ,  G01N33/6803 ,  G01N33/6842 ,  G01N33/6896 ,  G01N2440/36 ,  G01N2560/00 ,  Y10T436/24

    摘要： The invention provides a method detecting and quantifying proteins by mass spectrophotometric analysis using peptide internal standards and provides a highly sensitive way of detecting protein modifications. In one aspect, the invention provides a method for determining a site of ubiquitination in a polypeptide and for evaluating ubiquitination targets in a population of polypeptides. In this way, a proteome ubiquitination map can be obtained which comprises information relating to the ubiquitination states of a plurality of cellular polypeptides. Maps can be obtained for a variety of different types of cells and cell states. For example, ubiquitination targets in normal and diseased cells can be evaluated. Preferably, the map is stored as data files in a database. Individual ubiquitinated polypeptides identified can be used to generate molecular probes diagnostic of a cell state and/or can serve as targets for agents that modulate one or more cellular processes.

    摘要翻译： 本发明提供了通过使用肽内标的质谱分析法检测和定量蛋白质的方法，并提供了检测蛋白质修饰的高度灵敏的方法。 一方面，本发明提供了一种确定多肽泛素化位点的方法，并用于评价多肽群体中的泛素化靶标。 以这种方式，可以获得包含与多个细胞多肽的泛素化状态有关的信息的蛋白质组泛素化图。 可以获得各种不同类型的细胞和细胞状态的图。 例如，可以评估正常和患病细胞中的泛素化靶标。 优选地，地图作为数据文件存储在数据库中。 鉴定的单个泛素化多肽可用于产生诊断细胞状态的分子探针和/或可用作调节一种或多种细胞过程的试剂的靶标。