公开(公告)号：US12163191B2

公开(公告)日：2024-12-10

申请号：US16898984

申请日：2020-06-11

Applicant: 10X GENOMICS, INC.

Inventor： Michael Schnall-Levin ,  Mirna Jarosz ,  Christopher Hindson ,  Kevin Ness ,  Serge Saxonov ,  Benjamin Hindson ,  Xinying Zheng ,  Patrick Marks ,  John Stuelpnagel

IPC: C12Q1/6883 ,  C12Q1/6827 ,  G16B30/00 ,  G16B30/10 ,  G16B30/20

Abstract: The present disclosure relates to methods, compositions and systems for haplotype phasing and copy number variation assays. Included within this disclosure are methods and systems for combining the barcode comprising beads with samples in multiple separate partitions, as well as methods of processing, sequencing and analyzing barcoded samples.

公开(公告)号：US20240376519A1

公开(公告)日：2024-11-14

申请号：US18384527

申请日：2023-10-27

Applicant: 10X Genomics, Inc.

Inventor： Benjamin Hindson ,  Christopher Hindson ,  Michael Schnall-Levin ,  Kevin Ness ,  Mirna Jarosz ,  Donald Masquelier ,  Serge Saxonov ,  Landon Merrill ,  Andrew Price ,  Paul Hardenbol ,  Yuan Li

IPC: C12Q1/6806 ,  C12N15/10

Abstract: This disclosure provides methods and compositions for sample processing, particularly for sequencing applications. Included within this disclosure are bead compositions, such as diverse libraries of beads attached to large numbers of oligonucleotides containing barcodes. Often, the beads provides herein are degradable. For example, they may contain disulfide bonds that are susceptible to reducing agents. The methods provided herein include methods of making libraries of barcoded beads as well as methods of combining the beads with a sample, such as by using a microfluidic device.

公开(公告)号：US11591637B2

公开(公告)日：2023-02-28

申请号：US16056231

申请日：2018-08-06

Applicant: 10X Genomics, Inc.

Inventor： Benjamin Hindson ,  Christopher Hindson ,  Michael Schnall-Levin ,  Kevin Ness ,  Mirna Jarosz ,  Donald Masquelier ,  Serge Saxonov ,  Landon Merrill ,  Andrew Price ,  Paul Hardenbol ,  Yuan Li

IPC: C12Q1/68 ,  C12N15/10 ,  C12Q1/6806

Abstract: This disclosure provides methods and compositions for sample processing, particularly for sequencing applications. Included within this disclosure are bead compositions, such as diverse libraries of beads attached to large numbers of oligonucleotides containing barcodes. Often, the beads provides herein are degradable. For example, they may contain disulfide bonds that are susceptible to reducing agents. The methods provided herein include methods of making libraries of barcoded beads as well as methods of combining the beads with a sample, such as by using a microfluidic device.

公开(公告)号：US11473138B2

公开(公告)日：2022-10-18

申请号：US16698740

申请日：2019-11-27

Applicant: 10X GENOMICS, INC.

Inventor： Benjamin Hindson ,  Mirna Jarosz ,  Paul Hardenbol ,  Michael Schnall-Levin ,  Kevin Ness ,  Serge Saxonov

IPC: C12Q1/6874 ,  C12Q1/683 ,  C12N15/00 ,  C12N15/10 ,  C12Q1/6806

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing. In some cases, this disclosure provides methods for the generation of polynucleotide barcode libraries, and for the attachment of such polynucleotides to target polynucleotides.

公开(公告)号：US11278901B2

公开(公告)日：2022-03-22

申请号：US16887024

申请日：2020-05-29

Applicant: 10X Genomics, Inc.

Inventor： Rajiv Bharadwaj ,  Tobias Daniel Wheeler ,  Kevin Ness ,  Benjamin Hindson

IPC: B01L3/00 ,  B01J2/06 ,  C12Q1/6844 ,  B01F13/00 ,  B01F3/08

Abstract: A microfluidic emulsion droplet generation system and methods of use thereof are provided. The system may include a microfluidic substrate having a flow path configured and arranged for emulsion droplet generation, at least one textured surface in the flow path configured and arranged for inducing surface-mediated coalescence of emulsion droplets; and at least one channel junction in the flow path for emulsion droplet formation.

公开(公告)号：US20210277465A1

公开(公告)日：2021-09-09

申请号：US17314526

申请日：2021-05-07

Applicant: 10X GENOMICS, INC.

Inventor： Benjamin Hindson ,  Christopher Hindson ,  Michael Schnall-Levin ,  Kevin Ness ,  Mirna Jarosz ,  Serge Saxonov ,  Paul Hardenbol

IPC: C12Q1/6874 ,  C12Q1/6855 ,  C12Q1/6806 ,  C12Q1/6869

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

公开(公告)号：US20210246490A1

公开(公告)日：2021-08-12

申请号：US17195514

申请日：2021-03-08

Applicant: 10X Genomics, Inc.

Inventor： Xinying Zheng ,  Serge Saxonov ,  Michael Schnall-Levin ,  Kevin Ness ,  Rajiv Bharadwaj

IPC: C12Q1/6809 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6869

Abstract: The present invention is directed to methods, compositions and systems for analyzing sequence information while retaining structural and molecular context of that sequence information.

公开(公告)号：US20210123103A1

公开(公告)日：2021-04-29

申请号：US16898984

申请日：2020-06-11

Applicant: 10X GENOMICS, INC.

Inventor： Michael Schnall-Levin ,  Mirna Jarosz ,  Christopher Hindson ,  Kevin Ness ,  Serge Saxonov ,  Benjamin Hindson ,  Xinying Zheng ,  Patrick Marks ,  John Stuelpnagel

IPC: C12Q1/6883 ,  G16B30/00 ,  C12Q1/6827

Abstract: The present disclosure relates to methods, compositions and systems for haplotype phasing and copy number variation assays. Included within this disclosure are methods and systems for combining the barcode comprising beads with samples in multiple separate partitions, as well as methods of processing, sequencing and analyzing barcoded samples.

公开(公告)号：US10774370B2

公开(公告)日：2020-09-15

申请号：US15367660

申请日：2016-12-02

Applicant: 10X Genomics, Inc.

Inventor： Xinying Zheng ,  Serge Saxonov ,  Michael Schnall-Levin ,  Kevin Ness ,  Rajiv Bharadwaj

IPC: C12Q1/68 ,  C12Q1/6809 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6869

Abstract: The present invention is directed to methods, compositions and systems for analyzing sequence information while retaining structural and molecular context of that sequence information.

公开(公告)号：US10597718B2

公开(公告)日：2020-03-24

申请号：US16246322

申请日：2019-01-11

Applicant: 10X Genomics, Inc.

Inventor： Benjamin Hindson ,  Christopher Hindson ,  Michael Schnall-Levin ,  Kevin Ness ,  Mirna Jarosz ,  Serge Saxonov

IPC: C12Q1/68 ,  C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/6869

Abstract: This disclosure provides methods and compositions for sample processing, particularly for sequencing applications. Included within this disclosure are bead compositions, such as diverse beads provides herein are degradable. For libraries of beads attached to large numbers of oligonucleotides containing barcodes. Often, the example, they may contain disulfide bonds that are susceptible to reducing agents. The methods provided herein include methods of making libraries of barcoded beads as well as methods of combining the beads with a sample, such as by using a microfluidic device.