公开(公告)号：US12249402B2

公开(公告)日：2025-03-11

申请号：US18198763

申请日：2023-05-17

Applicant: 10x Genomics, Inc.

Inventor： Michael Schnall-Levin ,  Mirna Jarosz ,  Serge Saxonov ,  Kevin Ness ,  Benjamin Hindson

IPC: G16B30/10 ,  G16B30/00 ,  G16B30/20

Abstract: The invention described herein solves challenges in providing a proficient, rapid and meaningful analysis of sequencing data. Methods and computer program products of the invention allow for a system to receive, analyze, and display sequencing data in real-time. The invention provides solutions to several difficulties encountered in assembling short sequencing-reads, and by doing so the invention improves the worth and significance of sequencing data.

公开(公告)号：US12152278B2

公开(公告)日：2024-11-26

申请号：US17749050

申请日：2022-05-19

Applicant: 10x Genomics, Inc.

Inventor： Paul Ryvkin ,  Jason Underwood ,  Michael Schnall-Levin ,  Tarjei Mikkelsen ,  Benjamin Hindson

IPC: C12Q1/68 ,  C12Q1/6806 ,  C12Q1/6851 ,  C12Q1/6874

Abstract: Provided herein are compositions, systems and methods for tagging molecular events, reactions, species, etc., but without the need for complex, highly diverse libraries of tagging molecules. Provided are tagging moieties that can have a smaller number, a few, or even a single original “tagging” structure that may be transformed or transformable, in situ, into a collection of larger numbers of unique tagging or “barcode” moieties.

公开(公告)号：US20240376539A1

公开(公告)日：2024-11-14

申请号：US18386354

申请日：2023-11-02

Applicant: 10x Genomics, Inc.

Inventor： Benjamin Hindson ,  Serge Saxonov ,  Kevin Ness ,  Paul Hardenbol ,  Michael Schnall-Levin ,  Mirna Jarosz

IPC: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/6869

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

公开(公告)号：US12037634B2

公开(公告)日：2024-07-16

申请号：US17353202

申请日：2021-06-21

Applicant: 10X Genomics, Inc.

Inventor： Benjamin Hindson ,  Serge Saxonov ,  Michael Schnall-Levin

IPC: C12Q1/6806 ,  B01J19/00 ,  B01L3/00 ,  C12N15/10

CPC classification number: C12Q1/6806 ,  B01J19/0046 ,  B01L3/502715 ,  B01L3/508 ,  B01L3/523 ,  C12N15/1065 ,  B01L2200/0647 ,  B01L2400/0677 ,  C12Q2535/122 ,  C12Q2563/159 ,  C12Q1/6806 ,  C12Q2535/122 ,  C12Q2563/159

Abstract: This disclosure provides microwell capsule array devices. The microwell capsule array devices are generally capable of performing one or more sample preparation operations. Such sample preparation operations may be used as a prelude to one more or more analysis operations. For example, a device of this disclosure can achieve physical partitioning and discrete mixing of samples with unique molecular identifiers within a single unit in preparation for various analysis operations. The device may be useful in a variety of applications and most notably nucleic-acid-based sequencing, detection and quantification of gene expression and single-cell analysis.

公开(公告)号：US20220340968A1

公开(公告)日：2022-10-27

申请号：US17860880

申请日：2022-07-08

Applicant: 10x Genomics, Inc.

Inventor： Benjamin Hindson ,  Christopher Hindson ,  Michael Schnall-Levin ,  Kevin Ness ,  Mirna Jarosz ,  Serge Saxonov ,  Paul Hardenbol ,  Rajiv Bharadwaj ,  Xinying Zheng ,  Phillip Belgrader

IPC: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/683 ,  C12Q1/6804

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

公开(公告)号：US20210317524A1

公开(公告)日：2021-10-14

申请号：US17272033

申请日：2019-08-27

Applicant: 10x Genomics, Inc.

Inventor： Michael Ybarra Lucero ,  Michael Schnall-Levin ,  Tarjei Sigurd Mikkelsen ,  Eswar Prasad Ramachandran Iyer ,  Paul Giresi ,  Benjamin Hindson

IPC: C12Q1/6874

Abstract: Methods for determining a location of a feature in a spatial array of features include: providing a spatial array including a plurality of features on a substrate, where a feature of the plurality of features has a capture probe, and where the capture probe has a spatial barcode and a constant sequence; hybridizing a first sequencing probe to the spatial barcode, where the first sequencing probe has a first label and a first nucleotide sequence; obtaining a first image of the first label with the first sequencing probe hybridized to the spatial barcode; determining, based on the first image, a first portion of a sequence of the spatial barcode; and associating the feature with a location in the spatial array based on a location of the first label in the first image.

公开(公告)号：US11135584B2

公开(公告)日：2021-10-05

申请号：US16274134

申请日：2019-02-12

Applicant: 10X Genomics, Inc.

Inventor： Donald A. Masquelier ,  Benjamin Hindson ,  Kevin Ness ,  Rajiv Bharadwaj

IPC: B01L3/00 ,  F16K99/00 ,  B01L9/00 ,  C12Q1/6874

Abstract: An integrated system for processing and preparing samples for analysis may include a microfluidic device including a plurality of parallel channel networks for partitioning the samples including various fluids, and connected to a plurality of inlet and outlet reservoirs, at least a portion of the fluids comprising reagents, a holder including a closeable lid hingedly coupled thereto, in which in a closed configuration, the lid secures the microfluidic device in the holder, and in an open configuration, the lid is a stand orienting the microfluidic device at a desired angle to facilitate recovery of partitions or droplets from the partitioned samples generated within the microfluidic device, and an instrument configured to receive the holder and apply a pressure differential between the plurality of inlet and outlet reservoirs to drive fluid movement within the channel networks.

公开(公告)号：US11021749B2

公开(公告)日：2021-06-01

申请号：US16998414

申请日：2020-08-20

Applicant: 10X GENOMICS, INC.

Inventor： Benjamin Hindson ,  Kevin Ness ,  Serge Saxonov ,  Paul Hardenbol

IPC: C12Q1/68 ,  C12Q1/6874 ,  C12Q1/6855 ,  C12Q1/6806 ,  C12Q1/6869

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

公开(公告)号：US10688494B2

公开(公告)日：2020-06-23

申请号：US16325922

申请日：2017-08-23

Applicant: 10X Genomics, Inc.

Inventor： Rajiv Bharadwaj ,  Tobias Daniel Wheeler ,  Kevin Ness ,  Benjamin Hindson

IPC: C12Q1/68 ,  B01L3/00 ,  B01F13/00 ,  B01J2/06 ,  C12Q1/6844 ,  B01F3/08

Abstract: A microfluidic emulsion droplet generation system and methods of use thereof are provided. The system may include a microfluidic substrate having a flow path configured and arranged for emulsion droplet generation, at least one textured surface in the flow path configured and arranged for inducing surface-mediated coalescence of emulsion droplets; and at least one channel junction in the flow path for emulsion droplet formation.

公开(公告)号：US10533221B2

公开(公告)日：2020-01-14

申请号：US16165389

申请日：2018-10-19

Applicant: 10X GENOMICS, INC.

Inventor： Benjamin Hindson ,  Mirna Jarosz ,  Paul Hardenbol ,  Michael Schnall-Levin ,  Kevin Ness ,  Serge Saxonov

IPC: C12Q1/68 ,  C12Q1/6874 ,  C12Q1/683 ,  C12N15/10 ,  C12N15/00 ,  C12Q1/6806

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing. In some cases, this disclosure provides methods for the generation of polynucleotide barcode libraries, and for the attachment of such polynucleotides to target polynucleotides.