公开(公告)号：US20250027149A1

公开(公告)日：2025-01-23

申请号：US18760903

申请日：2024-07-01

Applicant: 10x Genomics, Inc.

Inventor： Benjamin Hindson ,  Christopher Hindson ,  Michael Schnall-Levin ,  Kevin Ness ,  Mirna Jarosz ,  Serge Saxonov

IPC: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/6869

Abstract: This disclosure provides methods and compositions for sample processing, particularly for sequencing applications. Included within this disclosure are bead compositions, such as diverse libraries of beads attached to large numbers of oligonucleotides containing barcodes. Often, the beads provides herein are degradable. For example, they may contain disulfide bonds that are susceptible to reducing agents. The methods provided herein include methods of making libraries of barcoded beads as well as methods of combining the beads with a sample, such as by using a microfluidic device.

公开(公告)号：US20240326052A1

公开(公告)日：2024-10-03

申请号：US18738471

申请日：2024-06-10

Applicant: 10x Genomics, Inc.

Inventor： Rajiv BHARADWAJ ,  Kevin Ness ,  Debkishore Mitra ,  Donald A. MASQUELIER ,  Anthony Makarewicz ,  Christopher Hindson ,  Benjamin Hindson ,  Serge Saxonov

IPC: B01L3/00 ,  B01L3/02 ,  G01N35/00 ,  G01N35/08 ,  G01N35/10

CPC classification number: B01L3/502761 ,  B01L3/0241 ,  B01L3/502784 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2400/049 ,  B01L2400/086 ,  G01N2035/00237 ,  G01N35/085 ,  G01N2035/1034

Abstract: The disclosure provides devices, systems and methods for the generation of encapsulated reagents and the partitioning of encapsulated reagents for use in subsequent analyses and/or processing, such as in the field of biological analyses and characterization.

公开(公告)号：US11713457B2

公开(公告)日：2023-08-01

申请号：US17986764

申请日：2022-11-14

Applicant: 10x Genomics, Inc.

Inventor： Benjamin Hindson ,  Christopher Hindson ,  Michael Schnall-Levin ,  Kevin Ness ,  Mirna Jarosz ,  Serge Saxonov

IPC: C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/683 ,  C12Q1/6874 ,  C12N15/10 ,  C40B50/16 ,  C40B20/04

CPC classification number: C12N15/1065 ,  C12Q1/683 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6874 ,  C40B20/04 ,  C40B50/16 ,  C12Q2525/191 ,  C12Q2535/122 ,  C12Q2537/143 ,  C12Q2537/149 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2563/179 ,  C12Q2565/629

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

公开(公告)号：US11629344B2

公开(公告)日：2023-04-18

申请号：US17860880

申请日：2022-07-08

Applicant: 10x Genomics, Inc.

Inventor： Benjamin Hindson ,  Christopher Hindson ,  Michael Schnall-Levin ,  Kevin Ness ,  Mirna Jarosz ,  Serge Saxonov

IPC: C12Q1/6874 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/683 ,  C12N15/10 ,  C40B50/16 ,  C40B20/04

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

公开(公告)号：US11084036B2

公开(公告)日：2021-08-10

申请号：US16180356

申请日：2018-11-05

Applicant: 10X GENOMICS, INC.

Inventor： Rajiv Bharadwaj ,  Kevin Ness ,  Tobias Daniel Wheeler

IPC: B01L3/00 ,  G01N1/28 ,  G01N33/487

Abstract: Microfluidic channels networks and systems are provided. One network includes a first fluid channel having a first depth dimension; at least a second channel intersecting the first channel at a first intersection; at least a third channel in fluid communication with the first intersection, at least one of the first intersection and the third channel having a depth dimension that is greater than the first depth dimension. Also provided is a flow control system for directing fluids in the network. Systems are additionally provided for flowing disrupted particles into a droplet formation junction, whereby a portion of the disrupted particles or the contents thereof are encapsulated into one or more droplets. Further provided is a method for controlling filling of a microfluidic network by controlling passive valving microfluidic channel network features.

公开(公告)号：US20200377942A1

公开(公告)日：2020-12-03

申请号：US16998414

申请日：2020-08-20

Applicant: 10X GENOMICS, INC.

Inventor： Benjamin Hindson ,  Christopher Hindson ,  Michael Schnall-Levin ,  Kevin Ness ,  Mirna Jarosz ,  Serge Saxonov ,  Paul Hardenbol ,  Rajiv Bharadwaj ,  Xinying Zheng ,  Phillip Belgrader

IPC: C12Q1/6874 ,  C12Q1/6855 ,  C12Q1/6806 ,  C12Q1/6869

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

公开(公告)号：US20200255894A1

公开(公告)日：2020-08-13

申请号：US16736323

申请日：2020-01-07

Applicant: 10X Genomics, Inc.

Inventor： Benjamin Hindson ,  Christopher Hindson ,  Michael Schnall-Levin ,  Kevin Ness ,  Mirna Jarosz ,  Serge Saxonov

IPC: C12Q1/6874 ,  C12Q1/6869 ,  C12Q1/6855 ,  C12Q1/6806

Abstract: This disclosure provides methods and compositions for sample processing, particularly for sequencing applications. Included within this disclosure are bead compositions, such as diverse libraries of beads attached to large numbers of oligonucleotides containing barcodes. Often, the beads provides herein are degradable. For example, they may contain disulfide bonds that are susceptible to reducing agents. The methods provided herein include methods of making libraries of barcoded beads as well as methods of combining the beads with a sample, such as by using a microfluidic device.

公开(公告)号：US20190203262A1

公开(公告)日：2019-07-04

申请号：US16242962

申请日：2019-01-08

Applicant: 10X Genomics, Inc.

Inventor： Benjamin Hindson ,  Christopher Hindson ,  Michael Schnall-Levin ,  Kevin Ness ,  Mirna Jarosz ,  Donald Masquelier ,  Serge Saxonov ,  Landon Merrill ,  Andrew Price ,  Paul Hardenbol ,  Yuan Li

IPC: C12Q1/6806 ,  C12N15/10

CPC classification number: C12Q1/6806 ,  B01J2219/005 ,  B01J2219/00547 ,  B01J2219/00572 ,  B01J2219/00722 ,  C12N15/1006 ,  C12N15/1065 ,  C12Q2535/122 ,  C12Q2563/159

Abstract: This disclosure provides methods and compositions for sample processing, particularly for sequencing applications. Included within this disclosure are bead compositions, such as diverse libraries of beads attached to large numbers of oligonucleotides containing barcodes. Often, the beads provides herein are degradable. For example, they may contain disulfide bonds that are susceptible to reducing agents. The methods provided herein include methods of making libraries of barcoded beads as well as methods of combining the beads with a sample, such as by using a microfluidic device.

公开(公告)号：US20190177788A1

公开(公告)日：2019-06-13

申请号：US16231185

申请日：2018-12-21

Applicant: 10X GENOMICS, INC.

Inventor： Benjamin Hindson ,  Christopher Hindson ,  Michael Schnall-Levin ,  Kevin Ness ,  Mirna Jarosz ,  Serge Saxonov ,  Paul Hardenbol ,  Rajiv Bharadwaj ,  Xinying Zheng ,  Phillip Belgrader

IPC: C12Q1/6874 ,  C12Q1/6869 ,  C12Q1/6855 ,  C12Q1/6806

CPC classification number: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/6869 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2563/179 ,  C12Q2565/519

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

公开(公告)号：US20190085380A1

公开(公告)日：2019-03-21

申请号：US16191343

申请日：2018-11-14

Applicant: 10X Genomics, Inc.

Inventor： Xinying Zheng ,  Serge Saxonov ,  Michael Schnall-Levin ,  Kevin Ness ,  Rajiv Bharadwaj

IPC: C12Q1/6809 ,  C12Q1/6869 ,  C12Q1/6834 ,  C12Q1/6806

Abstract: The present invention is directed to methods, compositions and systems for analyzing sequence information while retaining structural and molecular context of that sequence information.