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公开(公告)号：US20120273424A1
公开(公告)日：2012-11-01
申请号：US13457642
申请日：2012-04-27
申请人： Matthew C. Coffey , Allison Hagerman , Sarah Serl , Roxna Kapadia
发明人： Matthew C. Coffey , Allison Hagerman , Sarah Serl , Roxna Kapadia
CPC分类号： B01D15/34 , B01D15/426 , C12N1/02 , C12N7/00 , C12N2720/12051
摘要： Provided herein are elution buffers and methods for purifying viruses using gel permeation chromatography. The methods are useful, for example, in increasing recovery of a virus from a gel permeation chromatography column. The buffers for use in the methods include at least one excipient selected from histidine or sucrose, a divalent cation, a non-ionic detergent, and a phosphate buffered saline.
摘要翻译： 本文提供了洗脱缓冲液和使用凝胶渗透色谱法纯化病毒的方法。 该方法可用于例如从凝胶渗透色谱柱提高病毒的回收率。 用于该方法的缓冲液包括至少一种选自组氨酸或蔗糖的赋形剂，二价阳离子，非离子型洗涤剂和磷酸盐缓冲盐水。
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公开(公告)号：US08298785B2
公开(公告)日：2012-10-30
申请号：US12707307
申请日：2010-02-17
申请人： Matthew C. Coffey
发明人： Matthew C. Coffey
CPC分类号： C12Q1/04 , C12N15/1131 , C12N2310/121 , C12Q1/701 , C12Q2521/337
摘要： The present invention provides a method of detecting adventitious agents in a composition comprising a microorganism by using ribozyme-expressing indicator cells, as well as indicator cells useful in such detection.
摘要翻译： 本发明提供了通过使用表达核酶的指示细胞以及用于这种检测的指示细胞来检测包含微生物的组合物中的外源性物质的方法。
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公开(公告)号：US20120141528A1
公开(公告)日：2012-06-07
申请号：US13309901
申请日：2011-12-02
申请人： Matthew C. Coffey , Sarah Serl , Leo Pavliv
发明人： Matthew C. Coffey , Sarah Serl , Leo Pavliv
CPC分类号： A61K47/26 , A61K9/0019 , A61K9/19 , A61K35/00 , A61K35/76 , A61K35/765 , A61K35/768 , A61K47/02 , A61K47/183 , C12N7/00 , C12N2720/12051 , C12N2720/12251
摘要： Provided herein are lyophilized viral formulations useful for the stabilization and storage of viruses and methods of preparing these formulations. The lyophilized viral formulations described herein include a virus (e.g., a purified virus) and a non-viral composition including excipients. The formulations can be used, for example, to retain the infectivity or immunogenicity of viruses during periods of storage.
摘要翻译： 本文提供了可用于稳定和储存病毒的冻干病毒制剂和制备这些制剂的方法。 本文所述的冻干病毒制剂包括病毒（例如纯化病毒）和包含赋形剂的非病毒组合物。 该制剂可以用于例如在储存期间保留病毒的感染性或免疫原性。
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公开(公告)号：US07815914B2
公开(公告)日：2010-10-19
申请号：US11394609
申请日：2006-03-31
申请人： Matthew C. Coffey
发明人： Matthew C. Coffey
CPC分类号： G01N33/6872 , A61K35/765 , A61K45/06 , C12N2720/12232 , C12Q1/485 , G01N33/57407 , G01N33/57415 , G01N33/57496 , G01N2333/135 , G01N2333/14 , A61K2300/00
摘要： The present invention relates to methods of identifying the susceptibility of cells to reovirus infection by measuring constitutive ras-MAP signaling. The invention also pertains to methods using reovirus for the treatment of cellular proliferative disorders, and particularly cellular proliferative disorders wherein the proliferating cells exhibit constitutive MAPK phosphorylation, in mammals. In particular, the methods provide for reovirus treatment of mammals to treat proliferative disorders which include breast tumors, a subset of tumors in which mutation of the ras gene is not believed to play a significant role.
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公开(公告)号：US07476382B2
公开(公告)日：2009-01-13
申请号：US10836857
申请日：2004-04-30
CPC分类号： C12N9/1205 , A61K35/765 , C12N7/00 , C12N2720/12232 , C12N2720/12261
摘要： Methods for treating neoplasia, by administering reovirus to a Ras-mediated neoplasm, are disclosed. The reovirus is administered so that it ultimately directly contacts cells of the neoplasm. Human reovirus, non-human mammalian reovirus, and/or avian reovirus can be used. If the reovirus is human reovirus, type 1 (e.g., strain Lang), type 2 (e.g., strain Jones), type 3 (e.g., strain Dearing or strain Abney), as well as other serotypes or strains of reovirus can be used. Combinations of more than one type and/or strain of reovirus can be used, as can reovirus from different species of animal. Either solid neoplasms or hematopoietic neoplasms can be treated.
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公开(公告)号：US07223585B2
公开(公告)日：2007-05-29
申请号：US10424985
申请日：2003-04-29
申请人： Matthew C. Coffey
发明人： Matthew C. Coffey
CPC分类号： C12N7/00 , A61K39/12 , A61K39/15 , C07K14/005 , C12N2720/12051 , C12N2720/12222 , C12N2720/12234 , C12N2720/12251
摘要： The present invention is directed to an improved method of purifying virus, particularly reovirus. Infectious virus can be extracted from a cell culture with a detergent to produce high titers of virus, and the virus can then be purified by simple steps such as filtration and column chromatography. Viruses and compositions comprising the viruses prepared according to the present invention are also provided.
摘要翻译： 本发明涉及一种纯化病毒，特别是呼肠弧病毒的改进方法。 可以用洗涤剂从细胞培养物中提取传染病毒以产生高滴度的病毒，然后可以通过简单的步骤如过滤和柱层析纯化病毒。 还提供了包含根据本发明制备的病毒的病毒和组合物。
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公开(公告)号：US07052832B2
公开(公告)日：2006-05-30
申请号：US09985756
申请日：2001-11-06
申请人： Matthew C. Coffey
发明人： Matthew C. Coffey
IPC分类号： C12Q1/70 , G01N33/574
CPC分类号： G01N33/6872 , A61K35/765 , A61K45/06 , C12N2720/12232 , C12Q1/485 , G01N33/57407 , G01N33/57415 , G01N33/57496 , G01N2333/135 , G01N2333/14 , A61K2300/00
摘要： The present invention relates to methods of identifying the susceptibility of cells to reovirus infection by measuring constitutive ras-MAP signaling. The invention also pertains to methods using reovirus for the treatment of cellular proliferative disorders, and particularly cellular proliferative disorders wherein the proliferating cells exhibit constitutive MAPK phosphorylation, in mammals. In particular, the methods provide for reovirus treatment of mammals to treat proliferative disorders which include breast tumors, a subset of tumors in which mutation of the ras gene is not believed to play a significant role.
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公开(公告)号：US06811775B2
公开(公告)日：2004-11-02
申请号：US10218452
申请日：2002-08-15
IPC分类号： A61K3576
CPC分类号： A61K9/0019 , A61K35/765 , C12N2720/12032 , C12N2720/12232
摘要： Methods for treating proliferative disorders, by administering reovirus to a Ras-mediated proliferative disorder, are disclosed. The reovirus is administered so that it ultimately directly contacts ras-mediated proliferating cells. Proliferative disorders include but are not limited to neoplasms. Human reovirus, non-human mammalian reovirus, and/or avian reovirus can be used. If the reovirus is human reovirus, serotype 1 (e.g., strain Lang), serotype 2 (e.g., strain Jones), serotype 3 (e.g., strain Dearing or strain Abney), as well as other serotypes or strains of reovirus can be used. Combinations of more than one type and/or strain of reovirus can be used, as can reovirus from different species of animal. Either solid neoplasms or hematopoietic neoplasms can be treated.
摘要翻译： 公开了通过向Ras介导的增殖性病症施用呼肠孤病毒来治疗增殖性疾病的方法。 施用呼肠孤病毒使其最终直接接触ras介导的增殖细胞。 增生性疾病包括但不限于赘生物。 可以使用人呼肠病毒，非人哺乳动物呼肠病毒和/或禽呼肠孤病毒。 如果呼肠孤病毒是人呼肠病毒，则可以使用血清型1（例如，菌株Lang），血清型2（例如菌株琼斯），血清型3（例如，菌株Dearing或菌株Abney）以及呼肠孤病毒的其它血清型或菌株。 可以使用多于一种类型和/或菌株的呼肠病病毒的组合，以及来自不同动物种类的呼肠弧病毒。 可以治疗实体肿瘤或造血赘生物。
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公开(公告)号：US20130022964A1
公开(公告)日：2013-01-24
申请号：US13628499
申请日：2012-09-27
申请人： Matthew C. Coffey
发明人： Matthew C. Coffey
CPC分类号： C12Q1/04 , C12N15/1131 , C12N2310/121 , C12Q1/701 , C12Q2521/337
摘要： The present invention provides a method of detecting adventitious agents in a composition comprising a microorganism by using ribozyme-expressing indicator cells, as well as indicator cells useful in such detection.
摘要翻译： 本发明提供了通过使用表达核酶的指示细胞以及用于这种检测的指示细胞来检测包含微生物的组合物中的外源性物质的方法。
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公开(公告)号：US20110020288A1
公开(公告)日：2011-01-27
申请号：US12848684
申请日：2010-08-02
申请人： Matthew C. Coffey
发明人： Matthew C. Coffey
CPC分类号： A61K45/06 , A61K35/13 , A61K35/765 , C07K14/005 , C12N7/00 , C12N2720/12221 , C12N2720/12222
摘要： The invention provides for modified reovirus nucleic acid sequences and modified reovirus polypeptide sequences as well as reoviruses containing such modified nucleic acid or polypeptide sequences. The invention also provides for pharmaceutical compositions that include reoviruses having a modified sequence as well as methods of making and using such reoviruses.
摘要翻译： 本发明提供了修饰的呼肠孤病毒核酸序列和修饰的呼肠孤病毒多肽序列以及含有这种修饰的核酸或多肽序列的呼肠病毒。 本发明还提供了包含具有修饰序列的呼肠病毒的药物组合物以及制备和使用这些呼肠孤病毒的方法。
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