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    Method of extracting virus from cell culture
    1.
    发明授权
    Method of extracting virus from cell culture 有权
    从细胞培养中提取病毒的方法

    公开(公告)号：US06808916B2

    公开(公告)日：2004-10-26

    申请号：US10097183

    申请日：2002-03-14

    申请人： Matthew C. Coffey ,  Bradley G. Thompson

    发明人： Matthew C. Coffey ,  Bradley G. Thompson

    IPC分类号： C12N706

    CPC分类号： C12N7/00 ,  A61K35/765 ,  C12N2720/12232 ,  C12N2720/12251

    摘要： The present invention is directed to a method of extracting virus, particularly reovirus, from a culture of cells. Infectious virus can be extracted from the culture with a detergent at a convenient temperature such as 25° C. or 37° C. to produce high virus titers. Both ionic and non-ionic detergents can be used in the present invention.

    摘要翻译： 本发明涉及从细胞培养物中提取病毒，特别是呼肠弧病毒的方法。 感染性病毒可以用诸如25℃或37℃的方便温度的洗涤剂从培养物中提取，以产生高病毒效价。 离子和非离子洗涤剂都可用于本发明。

    REOVIRUS COMPOSITIONS AND METHODS OF USE
    4.
    发明申请
    REOVIRUS COMPOSITIONS AND METHODS OF USE 审中-公开
    REOVIRUS组合物和使用方法

    公开(公告)号：US20120251564A1

    公开(公告)日：2012-10-04

    申请号：US13443797

    申请日：2012-04-10

    申请人： Holly S. Sellers

    发明人： Holly S. Sellers

    IPC分类号： A61K39/15 ,  G01N33/569 ,  A61P31/20 ,  A61K39/295 ,  A61P31/14 ,  A61P37/04

    CPC分类号： C12N7/00 ,  A61K2039/525 ,  C07K14/005 ,  C12N2720/12222 ,  C12N2720/12251

    摘要： The present invention relates to novel strains of avian reovirus isolated from severe cases of Runting Stunting Syndrome in young broiler chickens in southeast United States. The invention is directed to avian reoviruses that impair digestion in poultry, diagnostic assays using nucleotide- or amino acid-specific components of such viruses, and to vaccines that protect chickens from disease caused by such viruses. Nucleotide sequences for the S1 gene, encoding the sigma C minor outer capsid protein, were amplified, and the nucleotide and predicted amino acid sequences were compared with sequences from other recently isolated reovirus field isolates and vaccine strains. Antigenic and molecular characterization of the newly isolated reoviruses revealed a lack of homogeneity with current U.S. isolates, with less than 60% percent amino acid similarity across the sigma C protein. Sequence comparisons with previously reported malabsorption isolates from Europe and Asia revealed a higher amino acid similarity, approaching 80%.

    摘要翻译： 本发明涉及从美国东南部的年轻肉鸡中严重狩猎发育不良综合症病例分离的禽呼肠孤病毒菌株。 本发明涉及损害家禽消化的禽类呼肠孤病毒，使用这种病毒的核苷酸或氨基酸特异性组分的诊断测定法，以及保护鸡免受由这种病毒引起的疾病的疫苗。 扩增编码σC次外侧衣壳蛋白的S1基因的核苷酸序列，并将核苷酸和预测的氨基酸序列与来自其他最近分离的呼肠孤病毒分离株和疫苗株的序列进行比较。 新分离的呼肠孤病毒的抗原和分子表征显示与目前的美国分离株缺乏同源性，其中σC蛋白的氨基酸相似性低于60％。 与以前报道的来自欧洲和亚洲的吸收分离物的序列比较显示出较高的氨基酸相似性，接近80％。

    REOVIRUS COMPOSITIONS AND METHODS OF USE
    6.
    发明申请
    REOVIRUS COMPOSITIONS AND METHODS OF USE 有权
    REOVIRUS组合物和使用方法

    公开(公告)号：US20100055131A1

    公开(公告)日：2010-03-04

    申请号：US12440762

    申请日：2007-10-25

    申请人： Holly S. Sellers

    发明人： Holly S. Sellers

    IPC分类号： A61K39/15 ,  C12N7/00 ,  A61K45/00 ,  G01N33/53 ,  A61P31/12 ,  A61P37/04

    CPC分类号： C12N7/00 ,  A61K2039/525 ,  C07K14/005 ,  C12N2720/12222 ,  C12N2720/12251

    摘要： The present invention relates to novel strains of avian reovirus that were isolated from severe cases of Runting Stunting Syndrome in young broiler chickens in southeast United States. The invention is directed to avian reoviruses that impair digestion in poultry, diagnostic assays using nucleotide- or amino acid-specific components of such viruses, and to vaccines that protect chickens from disease caused by such viruses. Nucleotide sequences for the SI gene, encoding the sigma C minor outer capsid protein, were amplified, and the nucleotide and predicted amino acid sequences were compared with sequences from other recently isolated reovirus field isolates and vaccine strains. Antigenic and molecular characterization of the newly isolated reoviruses revealed a lack of homogeneity with current U.S. isolates, with less than 60% percent amino acid similarity across the sigma C protein. Sequence comparisons with previously reported malabsorption isolates from Europe and Asia revealed a higher amino acid similarity, approaching 80%.

    摘要翻译： 本发明涉及从美国东南部的年轻肉鸡中严重的狩猎发育迟缓综合征病例中分离出的禽呼肠孤病毒菌株。 本发明涉及损害家禽消化的禽类呼肠孤病毒，使用这种病毒的核苷酸或氨基酸特异性组分的诊断测定法，以及保护鸡免受由这种病毒引起的疾病的疫苗。 扩增了编码σC次外侧衣壳蛋白的SI基因的核苷酸序列，并将核苷酸和预测的氨基酸序列与其他最近分离的呼肠孤病毒分离株和疫苗株的序列进行比较。 新分离的呼肠孤病毒的抗原和分子表征显示与目前的美国分离株缺乏同源性，其中σC蛋白的氨基酸相似性低于60％。 与以前报道的来自欧洲和亚洲的吸收分离物的序列比较显示出较高的氨基酸相似性，接近80％。

    Viral purification methods
    7.
    发明授权
    Viral purification methods 有权
    病毒纯化方法

    公开(公告)号：US07223585B2

    公开(公告)日：2007-05-29

    申请号：US10424985

    申请日：2003-04-29

    申请人： Matthew C. Coffey

    发明人： Matthew C. Coffey

    IPC分类号： C12N7/01 ,  C12N7/02 ,  A61K48/00

    CPC分类号： C12N7/00 ,  A61K39/12 ,  A61K39/15 ,  C07K14/005 ,  C12N2720/12051 ,  C12N2720/12222 ,  C12N2720/12234 ,  C12N2720/12251

    摘要： The present invention is directed to an improved method of purifying virus, particularly reovirus. Infectious virus can be extracted from a cell culture with a detergent to produce high titers of virus, and the virus can then be purified by simple steps such as filtration and column chromatography. Viruses and compositions comprising the viruses prepared according to the present invention are also provided.

    摘要翻译： 本发明涉及一种纯化病毒，特别是呼肠弧病毒的改进方法。 可以用洗涤剂从细胞培养物中提取传染病毒以产生高滴度的病毒，然后可以通过简单的步骤如过滤和柱层析纯化病毒。 还提供了包含根据本发明制备的病毒的病毒和组合物。

    LIQUID VIRAL FORMULATIONS
    10.
    发明申请
    LIQUID VIRAL FORMULATIONS 有权
    液体病毒制剂

    公开(公告)号：US20150224155A1

    公开(公告)日：2015-08-13

    申请号：US14693977

    申请日：2015-04-23

    申请人： ONCOLYTICS BIOTECH INC.

    发明人： Matthew C. Coffey ,  Sarah Serl ,  Leo Pavliv

    IPC分类号： A61K35/765 ,  C12N7/00

    CPC分类号： A61K35/765 ,  A61K35/76 ,  A61K39/15 ,  A61K2039/525 ,  C12N7/00 ,  C12N2720/12051 ,  C12N2720/12211 ,  C12N2720/12232 ,  C12N2720/12251

    摘要： Provided herein are liquid viral formulations useful for the stabilization and storage of viruses and methods of preparing these formulations. The liquid viral formulations described herein include a virus (e.g., a purified virus) and a non-viral composition including excipients and a liquid carrier. The formulations can be used, for example, to retain the infectivity or immunogenicity of viruses during periods of storage.

    摘要翻译： 本文提供了可用于稳定和储存病毒的液体病毒制剂和制备这些制剂的方法。 本文所述的液体病毒制剂包括病毒（例如，纯化病毒）和包含赋形剂和液体载体的非病毒组合物。 该制剂可以用于例如在储存期间保留病毒的感染性或免疫原性。