公开(公告)号：US10801037B2

公开(公告)日：2020-10-13

申请号：US16573538

申请日：2019-09-17

Applicant: Monsanto Technology LLC

Inventor： James A. Baum ,  Thomas Cerruti ,  Stanislaw Flasinski ,  Xiaoran Fu ,  Arlene R. Howe ,  Sara Ann Salvador

IPC: C12N15/82 ,  C07K14/325 ,  A01N63/10 ,  A01N47/08

Abstract: Engineered Cry1Da amino acid sequences are provided that exhibit improved Lepidopteran insecticidal activity and an enhanced Lepidopteran spectrum compared to the naturally occurring Cry1Da protein toxin. Polynucleotide sequences intended for use in expression of the improved proteins in plants are also provided. Particular embodiments provide compositions containing insect inhibitory amounts of the engineered proteins, as well as recombinant plants, plant parts, and seeds containing polynucleotide constructs encoding one or more of the improved engineered proteins.

公开(公告)号：US20200262876A1

公开(公告)日：2020-08-20

申请号：US16810810

申请日：2020-03-05

Applicant: Monsanto Technology LLC

Inventor： David J. Bowen ,  Catherine A. Chay ,  Arlene R. Howe ,  Uma Kesanapalli

IPC: C07K14/325 ,  C12Q1/6895 ,  C12N15/82

Abstract: Pesticidal proteins exhibiting toxic activity against Lepidopteran pest species are disclosed, and include, but are not limited to, TIC4472, TIC4472PL, TIC1425, TIC2613, and TIC2613PL. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding one or more of the disclosed pesticidal proteins. Transgenic plants, plant cells, seed, and plant parts resistant to Lepidopteran infestation are provided which contain recombinant nucleic acid sequences encoding the pesticidal proteins of the present invention. Methods for detecting the presence of the recombinant nucleic acid sequences or the proteins of the present invention in a biological sample, and methods of controlling Lepidopteran species pests using any of the TIC4472, TIC4472PL, TIC1425, TIC2613, and TIC2613PL pesticidal proteins are also provided.

公开(公告)号：US10662439B2

公开(公告)日：2020-05-26

申请号：US14945140

申请日：2015-11-18

Applicant: Monsanto Technology LLC

Inventor： Gregory J. Bean ,  David J. Bowen ,  Catherine A. Chay ,  Arlene R. Howe ,  Jason S. Milligan ,  Yong Yin

IPC: C12N15/82 ,  C07K14/195 ,  A01N63/20

Abstract: Insecticidal proteins exhibiting toxic activity against Coleopteran and Lepidopteran pest species are disclosed, and include, but are not limited to, TIC3668, TIC3669, TIC3670, TIC4076, TIC4078, TIC4260, TIC4346, TIC4826, TIC4861, TIC4862, TIC4863, and TIC-3668-type proteins. DNA molecules and constructs are provided which contain a polynucleotide sequence encoding one or more of the disclosed TIC3668-type proteins. Transgenic plants, plant cells, seed, and plant parts resistant to Lepidopteran and Coleopteran infestation are provided which contain polynucleotide sequences encoding the insecticidal proteins of the present invention. Methods for detecting the presence of the polynucleotides or the proteins of the present invention in a biological sample, and methods of controlling Coleopteran and Lepidopteran species pests using any of the TIC3668-type insecticidal proteins are also provided.

公开(公告)号：US10287605B2

公开(公告)日：2019-05-14

申请号：US15835346

申请日：2017-12-07

Applicant: Monsanto Technology LLC

Inventor： James A. Baum ,  Thomas Cerruti ,  Stanislaw Flasinski ,  Xiaoran Fu ,  Arlene R. Howe ,  Sara Ann Salvador

IPC: A01N47/08 ,  A01N63/02 ,  C12N15/82 ,  C07K14/325

Abstract: Engineered Cry1Da amino acid sequences are provided that exhibit improved Lepidopteran insecticidal activity and an enhanced Lepidopteran spectrum compared to the naturally occurring Cry1Da protein toxin. Polynucleotide sequences intended for use in expression of the improved proteins in plants are also provided. Particular embodiments provide compositions containing insect inhibitory amounts of the engineered proteins, as well as recombinant plants, plant parts, and seeds containing polynucleotide constructs encoding one or more of the improved engineered proteins.

公开(公告)号：US20180127775A1

公开(公告)日：2018-05-10

申请号：US15849218

申请日：2017-12-20

Applicant: Monsanto Technology LLC

Inventor： James A. Baum ,  Thomas A. Cerruti ,  Crystal L. Dart ,  Leigh H. English ,  Stanislaw Flasinski ,  Xiaoran Fu ,  Victor M. Guzov ,  Arlene R. Howe ,  Jay P. Morgenstern ,  James K. Roberts ,  Sara A. Salvador ,  Jinling Wang

IPC: C12N15/82

CPC classification number: C07K14/325 ,  A01N63/02 ,  C07K2319/00 ,  C12N15/62 ,  C12N15/8286 ,  Y02A40/162

Abstract: Nucleotide sequences are disclosed that encode novel chimeric insecticidal proteins exhibiting Lepidopteran inhibitory activity. Particular embodiments provide compositions and transformed plants, plant parts, and seeds containing the recombinant nucleic acid molecules encoding one or more of the chimeric insecticidal proteins.

公开(公告)号：US20180002386A1

公开(公告)日：2018-01-04

申请号：US15627164

申请日：2017-06-19

Applicant: Monsanto Technology LLC

Inventor： David J. Bowen ,  Catherine A. Chay ,  Arlene R. Howe ,  Jennifer L. Lutke

IPC: C07K14/32 ,  C12N15/82 ,  A01N63/02

CPC classification number: C12N15/8286 ,  A01N37/46 ,  A01N63/02 ,  C07K14/32 ,  Y02A40/162

Abstract: Nucleotide sequences are disclosed encoding novel, insecticidal TIC4747 and related proteins exhibiting Hemipteran and Lepidopteran inhibitory activity, as well as fragments thereof. Particular embodiments provide compositions and transformed plants, plant parts, and seeds containing a polynucleotide construct encoding one or more of the toxin proteins within the TIC4747-related protein toxin class.