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    METHOD OF PURIFYING THERAPEUTIC PROTEINS
    84.
    发明申请
    METHOD OF PURIFYING THERAPEUTIC PROTEINS 审中-公开

    公开(公告)号：US20190209948A1

    公开(公告)日：2019-07-11

    申请号：US16214519

    申请日：2018-12-10

    申请人： CSL Behring GmbH

    发明人： Hung Pham ,  Jeffrey Michael Hey ,  Darren Nguy

    IPC分类号： B01D15/36 ,  C07K14/755 ,  C07K14/745 ,  C07K14/75 ,  A61J1/05

    CPC分类号： B01D15/363 ,  A61J1/05 ,  A61K38/00 ,  C07K14/745 ,  C07K14/75 ,  C07K14/755

    摘要： The present invention relates generally to a method of reducing the level of at least one protein selected from the group consisting of plasminogen, tissue plasminogen activator and other protease(s) in a solution comprising at least one protein selected from the group consisting of fibrinogen, Factor VIII and von Willebrand factor (VWF), the method comprising: (i) passing a feedstock comprising at least one protein selected from the group consisting of fibrinogen, Factor VIII and VWF through a hydrophobic charge-induction chromatographic resin under conditions selected such that at least one protein selected from the group consisting of plasminogen, tissue plasminogen activator and other protease(s) present in the feedstock is bound to the resin; and (ii) recovering a solution comprising the at least one protein selected from the group consisting of fibrinogen, Factor VIII and VWF which passes through the resin, wherein the concentration of the at least one protein selected from the group consisting of plasminogen, tissue plasminogen activator and other protease(s) in the solution is reduced by at least 50% compared to the feedstock. Also provided are solutions and pharmaceutical formulations comprising the fibrinogen and/or Factor VIII and/or VWF recovered by such methods, and uses thereof.

    METHOD OF PURIFYING THERAPEUTIC PROTEINS
    88.
    发明申请
    METHOD OF PURIFYING THERAPEUTIC PROTEINS 审中-公开

    公开(公告)号：US20170143805A1

    公开(公告)日：2017-05-25

    申请号：US15424072

    申请日：2017-02-03

    申请人： CSL Behring GmbH

    发明人： Hung PHAM ,  Jeffrey Michael HEY ,  Darren NGUY

    IPC分类号： A61K38/36

    CPC分类号： B01D15/363 ,  A61J1/05 ,  A61K38/00 ,  A61K38/36 ,  A61K38/363 ,  C07K1/18 ,  C07K1/20 ,  C07K1/22 ,  C07K14/745 ,  C07K14/75 ,  C07K14/755

    摘要： The present invention relates generally to a method of reducing the level of at least one protein selected from the group consisting of plasminogen, tissue plasminogen activator and other protease(s) in a solution comprising at least one protein selected from the group consisting of fibrinogen, Factor VIII and von Willebrand factor (VWF), the method comprising: (i) passing a feedstock comprising at least one protein selected from the group consisting of fibrinogen, Factor VIII and VWF through a hydrophobic charge-induction chromatographic resin under conditions selected such that at least one protein selected from the group consisting of plasminogen, tissue plasminogen activator and other protease(s) present in the feedstock is bound to the resin; and (ii) recovering a solution comprising the at least one protein selected from the group consisting of fibrinogen, Factor VIII and VWF which passes through the resin, wherein the concentration of the at least one protein selected from the group consisting of plasminogen, tissue plasminogen activator and other protease(s) in the solution is reduced by at least 50% compared to the feedstock. Also provided are solutions and pharmaceutical formulations comprising the fibrinogen and/or Factor VIII and/or VWF recovered by such methods, and uses thereof.