公开(公告)号：US09598461B2

公开(公告)日：2017-03-21

申请号：US14535085

申请日：2014-11-06

申请人： CSL Behring GmbH

发明人： Hung Pham ,  Jeffrey Michael Hey ,  Darren Nguy

IPC分类号： C07K1/22 ,  C07K1/18 ,  C07K1/20 ,  C07K14/75 ,  A61K38/36 ,  C07K14/745 ,  C07K14/755

CPC分类号： A61K38/363 ,  A61K38/36 ,  C07K1/18 ,  C07K1/20 ,  C07K1/22 ,  C07K14/745 ,  C07K14/75 ,  C07K14/755

摘要： The present invention relates generally to a method of reducing the level of at least one protein selected from the group consisting of plasminogen, tissue plasminogen activator and other protease(s) in a solution comprising at least one protein selected from the group consisting of fibrinogen, Factor VIII and von Willebrand factor (VWF), the method comprising: (i) passing a feedstock comprising at least one protein selected from the group consisting of fibrinogen, Factor VIII and VWF through a hydrophobic charge-induction chromatographic resin under conditions selected such that at least one protein selected from the group consisting of plasminogen, tissue plasminogen activator and other protease(s) present in the feedstock is bound to the resin; and (ii) recovering a solution comprising the at least one protein selected from the group consisting of fibrinogen, Factor VIII and VWF which passes through the resin, wherein the concentration of the at least one protein selected from the group consisting of plasminogen, tissue plasminogen activator and other protease(s) in the solution is reduced by at least 50% compared to the feedstock. Also provided are solutions and pharmaceutical formulations comprising the fibrinogen and/or Factor VIII and/or VWF recovered by such methods, and uses thereof.

公开(公告)号：US11426680B2

公开(公告)日：2022-08-30

申请号：US16214519

申请日：2018-12-10

申请人： CSL Behring GmbH

发明人： Hung Pham ,  Jeffrey Michael Hey ,  Darren Nguy

IPC分类号： C07K14/75 ,  B01D15/36 ,  C07K1/20 ,  A61K38/36 ,  C07K14/745 ,  C07K1/18 ,  C07K1/22 ,  C07K14/755 ,  A61J1/05 ,  A61K38/00

摘要： The present invention relates generally to a method of reducing the level of at least one protein selected from the group consisting of plasminogen, tissue plasminogen activator and other protease(s) in a solution comprising at least one protein selected from the group consisting of fibrinogen, Factor VIII and von Willebrand factor (VWF), the method comprising: (i) passing a feedstock comprising at least one protein selected from the group consisting of fibrinogen, Factor VIII and VWF through a hydrophobic charge-induction chromatographic resin under conditions selected such that at least one protein selected from the group consisting of plasminogen, tissue plasminogen activator and other protease(s) present in the feedstock is bound to the resin; and (ii) recovering a solution comprising the at least one protein selected from the group consisting of fibrinogen, Factor VIII and VWF which passes through the resin, wherein the concentration of the at least one protein selected from the group consisting of plasminogen, tissue plasminogen activator and other protease(s) in the solution is reduced by at least 50% compared to the feedstock. Also provided are solutions and pharmaceutical formulations comprising the fibrinogen and/or Factor VIII and/or VWF recovered by such methods, and uses thereof.

公开(公告)号：US20190209948A1

公开(公告)日：2019-07-11

申请号：US16214519

申请日：2018-12-10

申请人： CSL Behring GmbH

发明人： Hung Pham ,  Jeffrey Michael Hey ,  Darren Nguy

IPC分类号： B01D15/36 ,  C07K14/755 ,  C07K14/745 ,  C07K14/75 ,  A61J1/05

CPC分类号： B01D15/363 ,  A61J1/05 ,  A61K38/00 ,  C07K14/745 ,  C07K14/75 ,  C07K14/755

摘要： The present invention relates generally to a method of reducing the level of at least one protein selected from the group consisting of plasminogen, tissue plasminogen activator and other protease(s) in a solution comprising at least one protein selected from the group consisting of fibrinogen, Factor VIII and von Willebrand factor (VWF), the method comprising: (i) passing a feedstock comprising at least one protein selected from the group consisting of fibrinogen, Factor VIII and VWF through a hydrophobic charge-induction chromatographic resin under conditions selected such that at least one protein selected from the group consisting of plasminogen, tissue plasminogen activator and other protease(s) present in the feedstock is bound to the resin; and (ii) recovering a solution comprising the at least one protein selected from the group consisting of fibrinogen, Factor VIII and VWF which passes through the resin, wherein the concentration of the at least one protein selected from the group consisting of plasminogen, tissue plasminogen activator and other protease(s) in the solution is reduced by at least 50% compared to the feedstock. Also provided are solutions and pharmaceutical formulations comprising the fibrinogen and/or Factor VIII and/or VWF recovered by such methods, and uses thereof.