公开(公告)号：US11365436B2

公开(公告)日：2022-06-21

申请号：US17075415

申请日：2020-10-20

申请人： Promega Corporation

发明人： Lance P. Encell ,  Mary Hall ,  Paul Otto ,  Gediminas Vidugiris ,  Keith V. Wood ,  Monika G. Wood ,  Kristopher Zimmerman

IPC分类号： C12Q1/66 ,  C12N9/00 ,  C12N9/02

摘要： A polynucleotide encoding a modified luciferase polypeptide. The modified luciferase polypeptide has at least 60% amino acid sequence identity to a wild-type Oplophorus luciferase and includes at least one amino acid substitution at a position corresponding to an amino acid in a wild-type Oplophorus luciferase of SEQ ID NO:1. The modified luciferase polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the wild-type Oplophorus luciferase.

公开(公告)号：US20210340593A1

公开(公告)日：2021-11-04

申请号：US17374460

申请日：2021-07-13

申请人： Promega Corporation

发明人： Carolyn W. Hitko ,  Thomas Kirkland ,  Sergiy Levin ,  Poncho Meisenheimer ,  Rachel Friedman Ohana ,  Harry Tetsuo Uyeda ,  Ji Zhu

IPC分类号： C12Q1/34 ,  G01N33/543 ,  C12N9/14

摘要： The present invention provides haloalkane substrates, and linkers for connecting such substrates to functional elements (e.g., tags, labels, surfaces, etc.). Substrates and linkers described herein find use, for example, in labeling, detection, and immobilization of proteins, cells, and molecules. In particular, the linkers provided herein find use within substrates for dehalogenase variants that form covalent bonds with their haloalkane substrates.

公开(公告)号：US20210254128A1

公开(公告)日：2021-08-19

申请号：US17240435

申请日：2021-04-26

申请人： PROMEGA CORPORATION

发明人： Anton Shakhmin ,  Thomas Kirkland ,  Joel Walker ,  Thomas Machleidt ,  Mary Hall ,  Keith V. Wood

IPC分类号： C12Q1/66 ,  C07D487/04 ,  C07D519/00 ,  A61K49/00 ,  G01N33/58 ,  H04N1/60

摘要： Described are substituted imidazo[1,2-a]pyrazine compounds, which are coelenterazine analogues, kits comprising the analogues, and methods of using the compounds for the detection of luminescence in luciferase-based assays. Also described are methods from making the compounds, such as a method using aminopyrazine acetophosphonates as synthesis intermediates.

公开(公告)号：US11066692B2

公开(公告)日：2021-07-20

申请号：US16137832

申请日：2018-09-21

申请人： PROMEGA CORPORATION

发明人： James J. Cali ,  Sarah Duellman ,  Jolanta Vidugiriene ,  Wenhui Zhou

IPC分类号： C12Q1/66 ,  G01N33/50

摘要： Provided herein are methods for the real-time monitoring of an intracellular event or response. In particular, the methods provided herein monitor the conversion of a pro-substrate to a substrate for a protein sensor as a result of an intracellular event or response.

公开(公告)号：US11015216B2

公开(公告)日：2021-05-25

申请号：US16865497

申请日：2020-05-04

申请人： PROMEGA CORPORATION

发明人： Anton Shakhmin ,  Thomas Kirkland ,  Joel Walker ,  Thomas Machleidt ,  Mary Hall ,  Keith V. Wood

IPC分类号： C07D487/04 ,  C12Q1/66 ,  C07D519/00 ,  A61K49/00 ,  G01N33/58 ,  H04N1/60

摘要： Described are substituted imidazo[1,2-a]pyrazine compounds, which are coelenterazine analogues, kits comprising the compounds, and methods of using the compounds for the detection of luminescence in luciferase-based assays. Also described are methods of making the compounds, such as a method using aminopyrazine acetophosphonates as synthesis intermediates. The substituted imidazo[1,2-a]pyrazine compounds include compounds of formula (I):

公开(公告)号：US20200292534A1

公开(公告)日：2020-09-17

申请号：US16851841

申请日：2020-04-17

申请人： Promega Corporation

发明人： Andrew S. Dixon ,  Lance Encell ,  Mary Hall ,  Keith Wood ,  Monika Wood ,  Marie Schwinn ,  Brock F. Binkowski ,  Hicham Zegzouti ,  Nidhi Nath ,  Subhanjan Mondal ,  Said Goueli ,  Poncho Meisenheimer ,  Thomas Kirkland ,  James Unch ,  Dileep K. Pulukkunat ,  Matthew Robers ,  Melanie Dart ,  Thomas Machleidt

IPC分类号： G01N33/533 ,  C07K14/435 ,  C12Q1/66 ,  C07K7/08 ,  C12N9/02 ,  G01N33/542 ,  G01N33/58 ,  A61K51/08

摘要： Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.

公开(公告)号：US20200263229A1

公开(公告)日：2020-08-20

申请号：US16865497

申请日：2020-05-04

申请人： PROMEGA CORPORATION

发明人： Anton Shakhmin ,  Thomas Kirkland ,  Joel Walker ,  Thomas Machleidt ,  Mary Hall ,  Keith V. Wood

IPC分类号： C12Q1/66 ,  H04N1/60 ,  G01N33/58 ,  A61K49/00 ,  C07D519/00 ,  C07D487/04

摘要： Described are substituted imidazo[1,2-a]pyrazine compounds, which are coelenterazine analogues, kits comprising the analogues, and methods of using the compounds for the detection of luminescence in luciferase-based assays. Also described are methods from making the compounds, such as a method using aminopyrazine acetophosphonates as synthesis intermediates.

公开(公告)号：US10648971B2

公开(公告)日：2020-05-12

申请号：US16035117

申请日：2018-07-13

申请人： Promega Corporation

发明人： Andrew S. Dixon ,  Lance Encell ,  Mary Hall ,  Keith Wood ,  Monika Wood ,  Marie Schwinn ,  Brock F. Binkowski ,  Hicham Zegzouti ,  Nidhi Nath ,  Subhanjan Mondal ,  Said Goueli ,  Poncho Meisenheimer ,  Thomas Kirkland ,  James Unch ,  Dileep K. Pulukkunat ,  Matthew Robers ,  Melanie Dart ,  Thomas Machleidt

IPC分类号： G01N31/00 ,  G01N33/53 ,  G01N33/533 ,  C07K14/435 ,  C12Q1/66 ,  C07K7/08 ,  C12N9/02 ,  G01N33/542 ,  G01N33/58 ,  A61K51/08

摘要： Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.

公开(公告)号：US20200042752A1

公开(公告)日：2020-02-06

申请号：US16531952

申请日：2019-08-05

申请人： Promega Corporation

发明人： Matt Tourdot ,  Brian George ,  Dillon Biermeier ,  John Kuehl

IPC分类号： G06K7/00 ,  G06K7/10

摘要： A system includes a processor, a barcode reader, and an RFID R/W component. The barcode reader scans a barcode of an item to generate barcode information, and communicates the barcode information to the processor. The RFID R/W component receives, from the processor, a serialization number corresponding to the barcode information. The RFID R/W component then writes the serialization number to an RFID tag of the item.

公开(公告)号：US20190338340A1

公开(公告)日：2019-11-07

申请号：US16516974

申请日：2019-07-19

申请人： Promega Corporation

发明人： Lance P. Encell ,  Mary P. Hall ,  Michael Killoran ,  Thomas A. Kirkland ,  Poncho Meisenheimer ,  Ce Shi

IPC分类号： C12Q1/66 ,  C07D513/04 ,  C07D513/10 ,  C07D277/66 ,  G01N21/76 ,  C07D417/04

摘要： Described are luciferin analogues, methods for making the analogues, kits including the analogues, and methods of using the compounds for the detection of luminescence in luciferase-based assays.