公开(公告)号：US20180095074A1

公开(公告)日：2018-04-05

申请号：US15717534

申请日：2017-09-27

申请人： Promega Corporation

发明人： Andrew S. Dixon ,  Lance Encell ,  Mary Hall ,  Keith Wood ,  Monika Wood ,  Marie Schwinn ,  Brock F. Binkowski ,  Hicham Zegzouti ,  Nidhi Nath ,  Subhanjan Mondal ,  Said Goueli ,  Poncho Meisenheimer ,  Thomas Kirkland ,  James Unch ,  Dileep K. Pulukkunat ,  Matthew Robers ,  Melanie Dart ,  Thomas Machleidt

IPC分类号： G01N33/533 ,  G01N33/58 ,  G01N33/542 ,  C12N9/02 ,  C07K14/435 ,  C07K7/08 ,  C12Q1/66 ,  A61K51/08

CPC分类号： G01N33/533 ,  A61K51/08 ,  C07K7/08 ,  C07K14/43509 ,  C12N9/0069 ,  C12Q1/66 ,  G01N33/542 ,  G01N33/581

摘要： Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.

公开(公告)号：US09869670B2

公开(公告)日：2018-01-16

申请号：US15073249

申请日：2016-03-17

申请人： Promega Corporation

发明人： Andrew S. Dixon ,  Lance Encell ,  Mary Hall ,  Keith Wood ,  Monika Wood ,  Marie Schwinn ,  Brock F. Binkowski ,  Hicham Zegzouti ,  Nidhi Nath ,  Subhanjan Mondal ,  Said Goueli ,  Poncho Meisenheimer ,  Thomas Kirkland ,  James Unch ,  Dileep K. Pulukkunat ,  Matthew Robers ,  Melanie Dart ,  Thomas Machleidt

IPC分类号： G01N31/00 ,  G01N33/53 ,  G01N33/533 ,  C07K14/435 ,  C12Q1/66 ,  C07K7/08 ,  A61K51/08 ,  C12N9/02 ,  G01N33/542 ,  G01N33/58

CPC分类号： G01N33/533 ,  A61K51/08 ,  C07K7/08 ,  C07K14/43509 ,  C12N9/0069 ,  C12Q1/66 ,  G01N33/542 ,  G01N33/581

摘要： Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.

公开(公告)号：US20140348747A1

公开(公告)日：2014-11-27

申请号：US14209546

申请日：2014-03-13

申请人： Promega Corporation

发明人： Andrew S. Dixon ,  Lance Encell ,  Mary Hall ,  Keith Wood ,  Monika Wood ,  Marie Schwinn ,  Brock F. Binkowski ,  Hicham Zegzouti ,  Nidhi Nath ,  Subhanjan Mondal ,  Said Goueli ,  Poncho Meisenheimer ,  Thomas Kirkland ,  James Unch ,  Dileep K. Pulukkunat ,  Matthew Robers ,  Melanie Dart ,  Thomas Machleidt

IPC分类号： G01N33/533 ,  A61K51/08

CPC分类号： G01N33/533 ,  A61K51/08 ,  C07K7/08 ,  C07K14/43509 ,  C12N9/0069 ,  C12Q1/66 ,  G01N33/542 ,  G01N33/581

摘要： Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.

公开(公告)号：US20190085305A1

公开(公告)日：2019-03-21

申请号：US15991695

申请日：2018-05-29

申请人： Promega Corporation

发明人： Aldis Darzins ,  Lance Encell ,  Tonny Johnson ,  Dieter Klaubert ,  Georgyi V. Los ,  Mark McDougall ,  Keith V. Wood ,  Monika G. Wood ,  Chad Zimprich

IPC分类号： C12N9/14 ,  B82Y30/00 ,  C12Q1/66 ,  C12Q1/26 ,  C12N9/02 ,  C12Q1/34 ,  B82Y10/00 ,  B82Y5/00

摘要： A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.

公开(公告)号：US09797890B2

公开(公告)日：2017-10-24

申请号：US14209610

申请日：2014-03-13

申请人： Promega Corporation

发明人： Andrew S. Dixon ,  Lance Encell ,  Mary Hall ,  Keith Wood ,  Monika Wood ,  Marie Schwinn ,  Brock F. Binkowski ,  Hicham Zegzouti ,  Nidhi Nath ,  Subhanjan Mondal ,  Said Goueli ,  Poncho Meisenheimer ,  Thomas Kirkland ,  James Unch ,  Dileep K. Pulukkunat ,  Matthew Robers ,  Melanie Dart ,  Thomas Machleidt

IPC分类号： C07K7/08 ,  C07K14/435 ,  C12Q1/66 ,  G01N33/533 ,  A61K51/08 ,  C12N9/02 ,  G01N33/542 ,  G01N33/58

CPC分类号： G01N33/533 ,  A61K51/08 ,  C07K7/08 ,  C07K14/43509 ,  C12N9/0069 ,  C12Q1/66 ,  G01N33/542 ,  G01N33/581

摘要： Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.

公开(公告)号：US20200292534A1

公开(公告)日：2020-09-17

申请号：US16851841

申请日：2020-04-17

申请人： Promega Corporation

发明人： Andrew S. Dixon ,  Lance Encell ,  Mary Hall ,  Keith Wood ,  Monika Wood ,  Marie Schwinn ,  Brock F. Binkowski ,  Hicham Zegzouti ,  Nidhi Nath ,  Subhanjan Mondal ,  Said Goueli ,  Poncho Meisenheimer ,  Thomas Kirkland ,  James Unch ,  Dileep K. Pulukkunat ,  Matthew Robers ,  Melanie Dart ,  Thomas Machleidt

IPC分类号： G01N33/533 ,  C07K14/435 ,  C12Q1/66 ,  C07K7/08 ,  C12N9/02 ,  G01N33/542 ,  G01N33/58 ,  A61K51/08

摘要： Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.

公开(公告)号：US10648971B2

公开(公告)日：2020-05-12

申请号：US16035117

申请日：2018-07-13

申请人： Promega Corporation

发明人： Andrew S. Dixon ,  Lance Encell ,  Mary Hall ,  Keith Wood ,  Monika Wood ,  Marie Schwinn ,  Brock F. Binkowski ,  Hicham Zegzouti ,  Nidhi Nath ,  Subhanjan Mondal ,  Said Goueli ,  Poncho Meisenheimer ,  Thomas Kirkland ,  James Unch ,  Dileep K. Pulukkunat ,  Matthew Robers ,  Melanie Dart ,  Thomas Machleidt

IPC分类号： G01N31/00 ,  G01N33/53 ,  G01N33/533 ,  C07K14/435 ,  C12Q1/66 ,  C07K7/08 ,  C12N9/02 ,  G01N33/542 ,  G01N33/58 ,  A61K51/08

摘要： Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.

公开(公告)号：US10288605B2

公开(公告)日：2019-05-14

申请号：US16023972

申请日：2018-06-29

申请人： Promega Corporation

发明人： Andrew S. Dixon ,  Lance Encell ,  Mary Hall ,  Keith Wood ,  Monika Wood ,  Marie Schwinn ,  Brock F. Binkowski ,  Hicham Zegzouti ,  Nidhi Nath ,  Subhanjan Mondal ,  Said Goueli ,  Poncho Meisenheimer ,  Thomas Kirkland ,  James Unch ,  Dileep K. Pulukkunat ,  Matthew Robers ,  Melanie Dart ,  Thomas Machleidt

IPC分类号： G01N31/00 ,  G01N33/53 ,  G01N33/533 ,  C07K14/435 ,  C12Q1/66 ,  C07K7/08 ,  A61K51/08 ,  C12N9/02 ,  G01N33/542 ,  G01N33/58

摘要： Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.

公开(公告)号：US20180196038A1

公开(公告)日：2018-07-12

申请号：US15899219

申请日：2018-02-19

申请人： Promega Corporation

发明人： Andrew S. Dixon ,  Lance Encell ,  Mary Hall ,  Keith Wood ,  Monika Wood ,  Marie Schwinn ,  Brock F. Binkowski ,  Hicham Zegzouti ,  Nidhi Nath ,  Subhanjan Mondal ,  Said Goueli ,  Poncho Meisenheimer ,  Thomas Kirkland ,  James Unch ,  Dileep K. Pulukkunat ,  Matthew Robers ,  Melanie Dart ,  Thomas Machleidt

IPC分类号： G01N33/533 ,  C07K14/435 ,  C12Q1/66 ,  C07K7/08 ,  G01N33/58 ,  A61K51/08 ,  C12N9/02 ,  G01N33/542

CPC分类号： G01N33/533 ,  A61K51/08 ,  C07K7/08 ,  C07K14/43509 ,  C12N9/0069 ,  C12Q1/66 ,  G01N33/542 ,  G01N33/581

摘要： Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.

公开(公告)号：US20160282340A1

公开(公告)日：2016-09-29

申请号：US15073249

申请日：2016-03-17

申请人： Promega Corporation

发明人： Andrew S. Dixon ,  Lance Encell ,  Mary Hall ,  Keith Wood ,  Monika Wood ,  Marie Schwinn ,  Brock F. Binkowski ,  Hicham Zegzouti ,  Nidhi Nath ,  Subhanjan Mondal ,  Said Goueli ,  Poncho Meisenheimer ,  Thomas Kirkland ,  James Unch ,  Dileep K. Pulukkunat ,  Matthew Robers ,  Melanie Dart ,  Thomas Machleidt

IPC分类号： G01N33/533

CPC分类号： G01N33/533 ,  A61K51/08 ,  C07K7/08 ,  C07K14/43509 ,  C12N9/0069 ,  C12Q1/66 ,  G01N33/542 ,  G01N33/581

摘要： Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.

摘要翻译： 本文提供了用于从两个或多个非发光（例如，基本上不发光的）肽和/或多肽单元组装生物发光复合物的组合物和方法。 特别地，通过与另一种互补的非发光肽的结构互补，赋予非发光多肽的生物发光活性。