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公开(公告)号：US11365402B2
公开(公告)日：2022-06-21
申请号：US15980406
申请日：2018-05-15
申请人： Promega Corporation
发明人： Andrew S. Dixon , Lance P. Encell , Thomas Machleidt , Marie Schwinn , Keith Wood , Monika Wood , Kris Zimmerman
IPC分类号： C12N9/14 , C07K14/315 , C07K14/475 , G01N33/533 , G01N33/535
摘要： Provided herein are substantially non-luminescent peptide/polypeptide tags that are inserted internally within a protein of interest or between N-terminal and C-terminal peptides/polypeptides. Interaction of the internally-inserted tag with a complement polypeptide/peptide that is also substantially non-luminescent results in the formation a bioluminescent reporter complex.
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公开(公告)号：US09797890B2
公开(公告)日：2017-10-24
申请号：US14209610
申请日：2014-03-13
申请人： Promega Corporation
发明人： Andrew S. Dixon , Lance Encell , Mary Hall , Keith Wood , Monika Wood , Marie Schwinn , Brock F. Binkowski , Hicham Zegzouti , Nidhi Nath , Subhanjan Mondal , Said Goueli , Poncho Meisenheimer , Thomas Kirkland , James Unch , Dileep K. Pulukkunat , Matthew Robers , Melanie Dart , Thomas Machleidt
IPC分类号： C07K7/08 , C07K14/435 , C12Q1/66 , G01N33/533 , A61K51/08 , C12N9/02 , G01N33/542 , G01N33/58
CPC分类号： G01N33/533 , A61K51/08 , C07K7/08 , C07K14/43509 , C12N9/0069 , C12Q1/66 , G01N33/542 , G01N33/581
摘要： Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.
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公开(公告)号：US10107800B2
公开(公告)日：2018-10-23
申请号：US15717534
申请日：2017-09-27
申请人： Promega Corporation
发明人： Andrew S. Dixon , Lance Encell , Mary Hall , Keith Wood , Monika Wood , Marie Schwinn , Brock F. Binkowski , Hicham Zegzouti , Nidhi Nath , Subhanjan Mondal , Said Goueli , Poncho Meisenheimer , Thomas Kirkland , James Unch , Dileep K. Pulukkunat , Matthew Robers , Melanie Dart , Thomas Machleidt
IPC分类号： G01N31/00 , G01N33/53 , G01N33/533 , G01N33/58 , G01N33/542 , C12N9/02 , A61K51/08 , C07K7/08 , C12Q1/66 , C07K14/435
摘要： Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.
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公开(公告)号：US09797889B2
公开(公告)日：2017-10-24
申请号：US14209546
申请日：2014-03-13
申请人： Promega Corporation
发明人： Andrew S. Dixon , Lance Encell , Mary Hall , Keith Wood , Monika Wood , Marie Schwinn , Brock F. Binkowski , Hicham Zegzouti , Nidhi Nath , Subhanjan Mondal , Said Goueli , Poncho Meisenheimer , Thomas Kirkland , James Unch , Dileep K. Pulukkunat , Matthew Robers , Melanie Dart , Thomas Machleidt
IPC分类号： C07K7/08 , C07K14/435 , C12Q1/66 , G01N33/533 , A61K51/08 , C12N9/02 , G01N33/542 , G01N33/58
CPC分类号： G01N33/533 , A61K51/08 , C07K7/08 , C07K14/43509 , C12N9/0069 , C12Q1/66 , G01N33/542 , G01N33/581
摘要： Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.
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公开(公告)号：US20160097042A1
公开(公告)日：2016-04-07
申请号：US14852096
申请日：2015-09-11
申请人： Promega Corporation
发明人： Andrew S. Dixon , Lance P. Encell , Thomas Machleidt , Marie Schwinn , Keith Wood , Monika Wood , Kris Zimmerman
IPC分类号： C12N9/14 , C12Q1/34 , C07K14/475 , C07K14/00 , C07K14/47
CPC分类号： C12N9/14 , C07K14/00 , C07K14/47 , C07K2319/60 , C07K2319/70 , C12Y308/01005
摘要： Provided herein are substantially non-luminescent peptide/polypeptide tags that are inserted internally within a protein of interest or between N-terminal and C-terminal peptides/polypeptides. Interaction of the internally-inserted tag with a complement polypeptide/peptide that is also substantially non-luminescent results in the formation a bioluminescent reporter complex).
摘要翻译： 本文提供了基本上不发光的肽/多肽标签，其内部插入目的蛋白质内或在N-末端和C-末端肽/多肽之间。 内部插入的标签与基本上不发光的补体多肽/肽的相互作用导致形成生物发光报告基因复合物）。
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公开(公告)号：US20210403886A9
公开(公告)日：2021-12-30
申请号：US15980406
申请日：2018-05-15
申请人： Promega Corporation
发明人： Andrew S. Dixon , Lance P. Encell , Thomas Machleidt , Marie Schwinn , Keith Wood , Monika Wood , Kris Zimmerman
IPC分类号： C12N9/14 , G01N33/535 , G01N33/533 , C07K14/315 , C07K14/475
摘要： Provided herein are substantially non-luminescent peptide/polypeptide tags that are inserted internally within a protein of interest or between N-terminal and C-terminal peptides/polypeptides. Interaction of the internally-inserted tag with a complement polypeptide/peptide that is also substantially non-luminescent results in the formation a bioluminescent reporter complex.
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公开(公告)号：US20190352623A1
公开(公告)日：2019-11-21
申请号：US15980406
申请日：2018-05-15
申请人： Promega Corporation
发明人： Andrew S. Dixon , Lance P. Encell , Thomas Machleidt , Marie Schwinn , Keith Wood , Monika Wood , Kris Zimmerman
IPC分类号： C12N9/14 , G01N33/535 , G01N33/533 , C07K14/315 , C07K14/475
摘要： Provided herein are substantially non-luminescent peptide/polypeptide tags that are inserted internally within a protein of interest or between N-terminal and C-terminal peptides/polypeptides. Interaction of the internally-inserted tag with a complement polypeptide/peptide that is also substantially non-luminescent results in the formation a bioluminescent reporter complex.
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公开(公告)号：US09969991B2
公开(公告)日：2018-05-15
申请号：US14852096
申请日：2015-09-11
申请人： Promega Corporation
发明人： Andrew S. Dixon , Lance P. Encell , Thomas Machleidt , Marie Schwinn , Keith Wood , Monika Wood , Kris Zimmerman
CPC分类号： C12N9/14 , C07K14/00 , C07K14/47 , C07K2319/60 , C07K2319/70 , C12Y308/01005
摘要： Provided herein are substantially non-luminescent peptide/polypeptide tags that are inserted internally within a protein of interest or between N-terminal and C-terminal peptides/polypeptides. Interaction of the internally-inserted tag with a complement polypeptide/peptide that is also substantially non-luminescent results in the formation a bioluminescent reporter complex.
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公开(公告)号：US20180095074A1
公开(公告)日：2018-04-05
申请号：US15717534
申请日：2017-09-27
申请人： Promega Corporation
发明人： Andrew S. Dixon , Lance Encell , Mary Hall , Keith Wood , Monika Wood , Marie Schwinn , Brock F. Binkowski , Hicham Zegzouti , Nidhi Nath , Subhanjan Mondal , Said Goueli , Poncho Meisenheimer , Thomas Kirkland , James Unch , Dileep K. Pulukkunat , Matthew Robers , Melanie Dart , Thomas Machleidt
IPC分类号： G01N33/533 , G01N33/58 , G01N33/542 , C12N9/02 , C07K14/435 , C07K7/08 , C12Q1/66 , A61K51/08
CPC分类号： G01N33/533 , A61K51/08 , C07K7/08 , C07K14/43509 , C12N9/0069 , C12Q1/66 , G01N33/542 , G01N33/581
摘要： Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.
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公开(公告)号：US09869670B2
公开(公告)日：2018-01-16
申请号：US15073249
申请日：2016-03-17
申请人： Promega Corporation
发明人： Andrew S. Dixon , Lance Encell , Mary Hall , Keith Wood , Monika Wood , Marie Schwinn , Brock F. Binkowski , Hicham Zegzouti , Nidhi Nath , Subhanjan Mondal , Said Goueli , Poncho Meisenheimer , Thomas Kirkland , James Unch , Dileep K. Pulukkunat , Matthew Robers , Melanie Dart , Thomas Machleidt
IPC分类号： G01N31/00 , G01N33/53 , G01N33/533 , C07K14/435 , C12Q1/66 , C07K7/08 , A61K51/08 , C12N9/02 , G01N33/542 , G01N33/58
CPC分类号： G01N33/533 , A61K51/08 , C07K7/08 , C07K14/43509 , C12N9/0069 , C12Q1/66 , G01N33/542 , G01N33/581
摘要： Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.
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